Tags

Type your tag names separated by a space and hit enter

Determination of biogenic amines in wines by pre-column derivatization and high-performance liquid chromatography coupled to mass spectrometry.
J Chromatogr A. 2009 Sep 04; 1216(36):6387-93.JC

Abstract

A new HPLC method for determining biogenic amines in wines is developed. This method is based on pre-column amine derivatization, further separation of derivatives and on-line hyphenation of HPLC to atmospheric pressure chemical ionization mass spectrometry (APCI-MS). Biogenic amines have been derivatized with 1,2-naphthoquinone-4-sulfonate at 65 degrees C and pH 9.2 for 5 min. The separation of derivatives has been accomplished in a C(18) analytical column using an elution gradient based on increasing the percentage of methanol. Derivatives have been ionized in positive mode and detected by selected ion monitoring. The operating conditions of the APCI-MS system (voltages, temperatures and gases) have been thoroughly optimized to obtain the maximum sensitivity for all analytes. In the selected conditions, APCI-MS spectra display little fragmentation and good signal-to-noise ratio. Depending on the amine characteristics, the main spectral peaks are due to mono- and di-derivative products. Figures of merit of the method have been established under the selected conditions using red wine samples. Recoveries ranging from 94% to 106% have been obtained which prove excellent accuracy of the method in the determination of histamine, putrescine, cadaverine, tryptamine, phenylethylamine, tyramine and serotonin in red wines. The proposed method has been applied to the analysis of commercial wines from different Spanish regions.

Authors+Show Affiliations

Department of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028-Barcelona, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19643426

Citation

García-Villar, Natividad, et al. "Determination of Biogenic Amines in Wines By Pre-column Derivatization and High-performance Liquid Chromatography Coupled to Mass Spectrometry." Journal of Chromatography. A, vol. 1216, no. 36, 2009, pp. 6387-93.
García-Villar N, Hernández-Cassou S, Saurina J. Determination of biogenic amines in wines by pre-column derivatization and high-performance liquid chromatography coupled to mass spectrometry. J Chromatogr A. 2009;1216(36):6387-93.
García-Villar, N., Hernández-Cassou, S., & Saurina, J. (2009). Determination of biogenic amines in wines by pre-column derivatization and high-performance liquid chromatography coupled to mass spectrometry. Journal of Chromatography. A, 1216(36), 6387-93. https://doi.org/10.1016/j.chroma.2009.07.029
García-Villar N, Hernández-Cassou S, Saurina J. Determination of Biogenic Amines in Wines By Pre-column Derivatization and High-performance Liquid Chromatography Coupled to Mass Spectrometry. J Chromatogr A. 2009 Sep 4;1216(36):6387-93. PubMed PMID: 19643426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines in wines by pre-column derivatization and high-performance liquid chromatography coupled to mass spectrometry. AU - García-Villar,Natividad, AU - Hernández-Cassou,Santiago, AU - Saurina,Javier, Y1 - 2009/07/18/ PY - 2009/03/20/received PY - 2009/07/09/revised PY - 2009/07/14/accepted PY - 2009/8/1/entrez PY - 2009/8/1/pubmed PY - 2009/12/30/medline SP - 6387 EP - 93 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 36 N2 - A new HPLC method for determining biogenic amines in wines is developed. This method is based on pre-column amine derivatization, further separation of derivatives and on-line hyphenation of HPLC to atmospheric pressure chemical ionization mass spectrometry (APCI-MS). Biogenic amines have been derivatized with 1,2-naphthoquinone-4-sulfonate at 65 degrees C and pH 9.2 for 5 min. The separation of derivatives has been accomplished in a C(18) analytical column using an elution gradient based on increasing the percentage of methanol. Derivatives have been ionized in positive mode and detected by selected ion monitoring. The operating conditions of the APCI-MS system (voltages, temperatures and gases) have been thoroughly optimized to obtain the maximum sensitivity for all analytes. In the selected conditions, APCI-MS spectra display little fragmentation and good signal-to-noise ratio. Depending on the amine characteristics, the main spectral peaks are due to mono- and di-derivative products. Figures of merit of the method have been established under the selected conditions using red wine samples. Recoveries ranging from 94% to 106% have been obtained which prove excellent accuracy of the method in the determination of histamine, putrescine, cadaverine, tryptamine, phenylethylamine, tyramine and serotonin in red wines. The proposed method has been applied to the analysis of commercial wines from different Spanish regions. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19643426/Determination_of_biogenic_amines_in_wines_by_pre_column_derivatization_and_high_performance_liquid_chromatography_coupled_to_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01079-6 DB - PRIME DP - Unbound Medicine ER -