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Ultrasensitive detection of indoleamines by combination of nanoparticle-based extraction with capillary electrophoresis/laser-induced native fluorescence.
J Chromatogr A. 2009 Sep 04; 1216(36):6451-8.JC

Abstract

For the first time, citrate-capped gold nanoparticles (citrate-AuNPs) have been used for the selective extraction of indoleamines--5-hydroxytryptophan (5-HTP), tryptophan (Trp), tryptamine (TA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA)--prior to their analysis by capillary electrophoresis/laser-induced native fluorescence (CE/LINF). The extinction spectra obtained for the citrate-AuNPs in the presence of indoleamines revealed that 5-HTP, 5-HT, and 5-HIAA were extracted mainly because of van der Waals interactions between the indole ring and the citrate-AuNPs (hydrophobic surface), while 5-HT and TA were extracted by electrostatic attractions between the amine group of the indoleamines and the citrate ligands adsorbed on the AuNP surface. The extracted indoleamines could be liberated from the AuNP surface by the addition of high concentrations of 2-mercaptoethanol (2-ME), which binds strongly to the AuNPs. The sensitivity of this method to indoleamines could be significantly enhanced by increasing the AuNP concentration, incubation time, and sample volume. Under optimal extraction and separation conditions, the combination of NP-based extraction and CE-LINF provided 48-, 4077-, 985-, 920-, and 4030-fold improvements in the limits of detection (signal-to-noise ratio of 3) for 5-HTP, Trp, TA, 5-HT, and 5-HIAA as compared to the analysis of five indoleamines by CE-LINF. In addition, this proposed method was successfully used for the determination of TA and 5-HT in urine.

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Authors+Show Affiliations

Li MD
Department of Chemistry, National Sun Yat-sen University, 70, Lien-hai Road, Kaohsiung, Taiwan.
Tseng WL
No affiliation info available
Cheng TL
No affiliation info available

MeSH

5-HydroxytryptophanAdsorptionCitric AcidDextran SulfateElectrophoresis, CapillaryHumansHydroxyindoleacetic AcidLasersLightLinear ModelsMaleMercaptoethanolMetal NanoparticlesSerotoninSpectrometry, FluorescenceSurface Plasmon ResonanceSurface PropertiesTryptaminesTryptophanYoung Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19646710

Citation

Li, Mu-De, et al. "Ultrasensitive Detection of Indoleamines By Combination of Nanoparticle-based Extraction With Capillary Electrophoresis/laser-induced Native Fluorescence." Journal of Chromatography. A, vol. 1216, no. 36, 2009, pp. 6451-8.
Li MD, Tseng WL, Cheng TL. Ultrasensitive detection of indoleamines by combination of nanoparticle-based extraction with capillary electrophoresis/laser-induced native fluorescence. J Chromatogr A. 2009;1216(36):6451-8.
Li, M. D., Tseng, W. L., & Cheng, T. L. (2009). Ultrasensitive detection of indoleamines by combination of nanoparticle-based extraction with capillary electrophoresis/laser-induced native fluorescence. Journal of Chromatography. A, 1216(36), 6451-8. https://doi.org/10.1016/j.chroma.2009.07.034
Li MD, Tseng WL, Cheng TL. Ultrasensitive Detection of Indoleamines By Combination of Nanoparticle-based Extraction With Capillary Electrophoresis/laser-induced Native Fluorescence. J Chromatogr A. 2009 Sep 4;1216(36):6451-8. PubMed PMID: 19646710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive detection of indoleamines by combination of nanoparticle-based extraction with capillary electrophoresis/laser-induced native fluorescence. AU - Li,Mu-De, AU - Tseng,Wei-Lung, AU - Cheng,Tian-Lu, Y1 - 2009/07/21/ PY - 2009/04/02/received PY - 2009/07/11/revised PY - 2009/07/17/accepted PY - 2009/8/4/entrez PY - 2009/8/4/pubmed PY - 2009/12/30/medline SP - 6451 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 36 N2 - For the first time, citrate-capped gold nanoparticles (citrate-AuNPs) have been used for the selective extraction of indoleamines--5-hydroxytryptophan (5-HTP), tryptophan (Trp), tryptamine (TA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA)--prior to their analysis by capillary electrophoresis/laser-induced native fluorescence (CE/LINF). The extinction spectra obtained for the citrate-AuNPs in the presence of indoleamines revealed that 5-HTP, 5-HT, and 5-HIAA were extracted mainly because of van der Waals interactions between the indole ring and the citrate-AuNPs (hydrophobic surface), while 5-HT and TA were extracted by electrostatic attractions between the amine group of the indoleamines and the citrate ligands adsorbed on the AuNP surface. The extracted indoleamines could be liberated from the AuNP surface by the addition of high concentrations of 2-mercaptoethanol (2-ME), which binds strongly to the AuNPs. The sensitivity of this method to indoleamines could be significantly enhanced by increasing the AuNP concentration, incubation time, and sample volume. Under optimal extraction and separation conditions, the combination of NP-based extraction and CE-LINF provided 48-, 4077-, 985-, 920-, and 4030-fold improvements in the limits of detection (signal-to-noise ratio of 3) for 5-HTP, Trp, TA, 5-HT, and 5-HIAA as compared to the analysis of five indoleamines by CE-LINF. In addition, this proposed method was successfully used for the determination of TA and 5-HT in urine. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19646710/Ultrasensitive_detection_of_indoleamines_by_combination_of_nanoparticle_based_extraction_with_capillary_electrophoresis/laser_induced_native_fluorescence_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01085-1 DB - PRIME DP - Unbound Medicine ER -