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Establishing a public health analytical service based on chemical methods for detecting and quantifying Pacific ciguatoxin in fish samples.
Toxicon. 2010 Oct; 56(5):804-12.T

Abstract

A referee analysis method for the detection and quantification of Pacific ciguatoxins in fish flesh has recently been established by the public health analytical laboratory for the State of Queensland, Australia. Fifty-six fish samples were analysed, which included 10 fillets purchased as negative controls. P-CTX-1 was identified in 27 samples, and P-CTX-2 and P-CTX-3 were found in 26 of those samples. The range of P-CTX-1 concentrations was 0.04-11.4 microg/kg fish flesh; coefficient of variation from 90 replicate analyses was 7.4%. A liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method utilising a rapid methanol extraction and clean-up is reliable and reproducible, with the detection limit at 0.03 microg/kg fish flesh. Some matrix effects are evident, with fish oil content a likely signal suppression factor. Species identification of samples by DNA sequence analysis revealed some evidence of fish substitution or inadvertent misidentification, which may have implications for the management and prevention of ciguatera poisoning. Blinded inspection of case notes from suspect ciguatera poisoning cases showed that reporting of ciguatera-related paraesthesias was highly predictable for the presence of ciguatoxins in analysed fish, with 13 of 14 expected cases having consumed fish that contained P-CTX-1 (p<0.001, Fishers Exact Test).

Authors+Show Affiliations

Queensland Health Forensic and Scientific Services, Coopers Plains, Queensland 4108, Australia. ian.stewart@griffith.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19647011

Citation

Stewart, Ian, et al. "Establishing a Public Health Analytical Service Based On Chemical Methods for Detecting and Quantifying Pacific Ciguatoxin in Fish Samples." Toxicon : Official Journal of the International Society On Toxinology, vol. 56, no. 5, 2010, pp. 804-12.
Stewart I, Eaglesham GK, Poole S, et al. Establishing a public health analytical service based on chemical methods for detecting and quantifying Pacific ciguatoxin in fish samples. Toxicon. 2010;56(5):804-12.
Stewart, I., Eaglesham, G. K., Poole, S., Graham, G., Paulo, C., Wickramasinghe, W., Sadler, R., & Shaw, G. R. (2010). Establishing a public health analytical service based on chemical methods for detecting and quantifying Pacific ciguatoxin in fish samples. Toxicon : Official Journal of the International Society On Toxinology, 56(5), 804-12. https://doi.org/10.1016/j.toxicon.2009.07.028
Stewart I, et al. Establishing a Public Health Analytical Service Based On Chemical Methods for Detecting and Quantifying Pacific Ciguatoxin in Fish Samples. Toxicon. 2010;56(5):804-12. PubMed PMID: 19647011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishing a public health analytical service based on chemical methods for detecting and quantifying Pacific ciguatoxin in fish samples. AU - Stewart,Ian, AU - Eaglesham,Geoffrey K, AU - Poole,Sue, AU - Graham,Glenn, AU - Paulo,Carl, AU - Wickramasinghe,Wasantha, AU - Sadler,Ross, AU - Shaw,Glen R, Y1 - 2009/07/30/ PY - 2009/01/15/received PY - 2009/07/13/revised PY - 2009/07/21/accepted PY - 2009/8/4/entrez PY - 2009/8/4/pubmed PY - 2010/12/25/medline SP - 804 EP - 12 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 56 IS - 5 N2 - A referee analysis method for the detection and quantification of Pacific ciguatoxins in fish flesh has recently been established by the public health analytical laboratory for the State of Queensland, Australia. Fifty-six fish samples were analysed, which included 10 fillets purchased as negative controls. P-CTX-1 was identified in 27 samples, and P-CTX-2 and P-CTX-3 were found in 26 of those samples. The range of P-CTX-1 concentrations was 0.04-11.4 microg/kg fish flesh; coefficient of variation from 90 replicate analyses was 7.4%. A liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method utilising a rapid methanol extraction and clean-up is reliable and reproducible, with the detection limit at 0.03 microg/kg fish flesh. Some matrix effects are evident, with fish oil content a likely signal suppression factor. Species identification of samples by DNA sequence analysis revealed some evidence of fish substitution or inadvertent misidentification, which may have implications for the management and prevention of ciguatera poisoning. Blinded inspection of case notes from suspect ciguatera poisoning cases showed that reporting of ciguatera-related paraesthesias was highly predictable for the presence of ciguatoxins in analysed fish, with 13 of 14 expected cases having consumed fish that contained P-CTX-1 (p<0.001, Fishers Exact Test). SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/19647011/Establishing_a_public_health_analytical_service_based_on_chemical_methods_for_detecting_and_quantifying_Pacific_ciguatoxin_in_fish_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(09)00379-1 DB - PRIME DP - Unbound Medicine ER -