Tags

Type your tag names separated by a space and hit enter

Antioxidant activity of Acanthopanax trifoliatus.
Med Princ Pract. 2009; 18(5):393-8.MP

Abstract

OBJECTIVE

The aim of this study was to investigate the antioxidant activity of extracts of various parts of Acanthopanax trifoliatus obtained by different extraction methods. The total phenolic and flavonoid contents of the extracts were also determined.

MATERIALS AND METHODS

The leaves, stems, stem bark, roots and root bark of A. trifoliatus were extracted separately using decoction, maceration and refluxing methods. The extracts were analysed for free-radical-scavenging activity using the 1,1-diphenyl-2-picryl-hydrazyl scavenging assay and the thiobarbituric-acid-reactive substance method for the inhibition of lipid peroxidation of a rat brain homogenate. Total phenolic and total flavonoid contents of the extracts were measured by UV spectrophotometry.

RESULTS

The leaf decoction extracts possessed a significantly stronger antioxidant activity as revealed by both methods. Total phenolic and flavonoid contents of the extracts ranged from 2.16 to 21.79 g% chlorogenic acid equivalent and from 0.37 to 9.61 g% rutin equivalent, respectively. Analysis of the leaf decoction extract, which exhibited the most potent antioxidant activity, by thin-layer chromatography revealed flavonoid and polyphenolic compounds corresponding to rutin and chlorogenic acid.

CONCLUSION

The leaf aqueous extracts showed a high level of antioxidative activity and contained high levels of both phenolic and flavonoid compounds. The magnitude of antioxidant activity corresponded with the level of phenolic and flavonoid compounds.

Links

Publisher Full Text
karger.com?DOI=10.1159
karger.com?DOI=10.1159

Authors+Show Affiliations

Sithisarn P
Pharmaceutical and Natural Products Department, Thailand Institute of Scientific and Technological Research, Technopolis, Klong Luang, Pathumthani, Thailand.
Jarikasem S
No affiliation info available

MeSH

Chromatography, Thin LayerEleutherococcusFlavonoidsFree Radical ScavengersMedicine, East Asian TraditionalPhenolsPlant BarkPlant ExtractsPlant LeavesPlant Stems

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19648763

Citation

Sithisarn, Pongtip, and Siripen Jarikasem. "Antioxidant Activity of Acanthopanax Trifoliatus." Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, vol. 18, no. 5, 2009, pp. 393-8.
Sithisarn P, Jarikasem S. Antioxidant activity of Acanthopanax trifoliatus. Med Princ Pract. 2009;18(5):393-8.
Sithisarn, P., & Jarikasem, S. (2009). Antioxidant activity of Acanthopanax trifoliatus. Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre, 18(5), 393-8. https://doi.org/10.1159/000226294
Sithisarn P, Jarikasem S. Antioxidant Activity of Acanthopanax Trifoliatus. Med Princ Pract. 2009;18(5):393-8. PubMed PMID: 19648763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity of Acanthopanax trifoliatus. AU - Sithisarn,Pongtip, AU - Jarikasem,Siripen, Y1 - 2009/07/31/ PY - 2008/08/06/received PY - 2008/11/11/accepted PY - 2009/8/4/entrez PY - 2009/8/4/pubmed PY - 2009/10/29/medline SP - 393 EP - 8 JF - Medical principles and practice : international journal of the Kuwait University, Health Science Centre JO - Med Princ Pract VL - 18 IS - 5 N2 - OBJECTIVE: The aim of this study was to investigate the antioxidant activity of extracts of various parts of Acanthopanax trifoliatus obtained by different extraction methods. The total phenolic and flavonoid contents of the extracts were also determined. MATERIALS AND METHODS: The leaves, stems, stem bark, roots and root bark of A. trifoliatus were extracted separately using decoction, maceration and refluxing methods. The extracts were analysed for free-radical-scavenging activity using the 1,1-diphenyl-2-picryl-hydrazyl scavenging assay and the thiobarbituric-acid-reactive substance method for the inhibition of lipid peroxidation of a rat brain homogenate. Total phenolic and total flavonoid contents of the extracts were measured by UV spectrophotometry. RESULTS: The leaf decoction extracts possessed a significantly stronger antioxidant activity as revealed by both methods. Total phenolic and flavonoid contents of the extracts ranged from 2.16 to 21.79 g% chlorogenic acid equivalent and from 0.37 to 9.61 g% rutin equivalent, respectively. Analysis of the leaf decoction extract, which exhibited the most potent antioxidant activity, by thin-layer chromatography revealed flavonoid and polyphenolic compounds corresponding to rutin and chlorogenic acid. CONCLUSION: The leaf aqueous extracts showed a high level of antioxidative activity and contained high levels of both phenolic and flavonoid compounds. The magnitude of antioxidant activity corresponded with the level of phenolic and flavonoid compounds. SN - 1423-0151 UR - https://www.unboundmedicine.com/medline/citation/19648763/Antioxidant_activity_of_Acanthopanax_trifoliatus_ L2 - https://www.karger.com?DOI=10.1159/000226294 DB - PRIME DP - Unbound Medicine ER -