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A decreased frequency of regulatory T cells in patients with common variable immunodeficiency.
PLoS One. 2009 Jul 29; 4(7):e6269.Plos

Abstract

INTRODUCTION

Common variable immunodeficiency disorder (CVID) is a heterogeneous syndrome, characterized by deficient antibody production and recurrent bacterial infections in addition abnormalities in T cells. CD4(+)CD25(high) regulatory T cells (Treg) are essential modulators of immune responses, including down-modulation of immune response to pathogens, allergens, cancer cells and self-antigens.

OBJECTIVE

In this study we set out to investigate the frequency of Treg cells in CVID patients and correlate with their immune activation status.

MATERIALS AND METHODS

Sixteen patients (6 males and 10 females) with CVID who had been treated with regular intravenous immunoglobulin and 14 controls were enrolled. Quantitative analyses of peripheral blood mononuclear cells (PBMC) were performed by multiparametric flow cytometry using the following cell markers: CD38, HLA-DR, CCR5 (immune activation); CD4, CD25, FOXP3, CD127, and OX40 (Treg cells); Ki-67 and IFN-gamma (intracellular cytokine).

RESULTS

A significantly lower proportion of CD4(+)CD25(high)FOXP3 T cells was observed in CVID patients compared with healthy controls (P<0.05). In addition to a higher proportion of CD8(+) T cells from CVID patients expressing the activation markers, CD38(+) and HLA-DR(+) (P<0.05), we observed no significant correlation between Tregs and immune activation.

CONCLUSION

Our results demonstrate that a reduction in Treg cells could have impaired immune function in CVID patients.

Authors+Show Affiliations

Federal University of São Paulo, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19649263

Citation

Melo, Karina M., et al. "A Decreased Frequency of Regulatory T Cells in Patients With Common Variable Immunodeficiency." PloS One, vol. 4, no. 7, 2009, pp. e6269.
Melo KM, Carvalho KI, Bruno FR, et al. A decreased frequency of regulatory T cells in patients with common variable immunodeficiency. PLoS ONE. 2009;4(7):e6269.
Melo, K. M., Carvalho, K. I., Bruno, F. R., Ndhlovu, L. C., Ballan, W. M., Nixon, D. F., Kallas, E. G., & Costa-Carvalho, B. T. (2009). A decreased frequency of regulatory T cells in patients with common variable immunodeficiency. PloS One, 4(7), e6269. https://doi.org/10.1371/journal.pone.0006269
Melo KM, et al. A Decreased Frequency of Regulatory T Cells in Patients With Common Variable Immunodeficiency. PLoS ONE. 2009 Jul 29;4(7):e6269. PubMed PMID: 19649263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A decreased frequency of regulatory T cells in patients with common variable immunodeficiency. AU - Melo,Karina M, AU - Carvalho,Karina I, AU - Bruno,Fernanda R, AU - Ndhlovu,Lishomwa C, AU - Ballan,Wassim M, AU - Nixon,Douglas F, AU - Kallas,Esper G, AU - Costa-Carvalho,Beatriz T, Y1 - 2009/07/29/ PY - 2009/03/09/received PY - 2009/06/02/accepted PY - 2009/8/4/entrez PY - 2009/8/4/pubmed PY - 2009/11/11/medline SP - e6269 EP - e6269 JF - PloS one JO - PLoS ONE VL - 4 IS - 7 N2 - INTRODUCTION: Common variable immunodeficiency disorder (CVID) is a heterogeneous syndrome, characterized by deficient antibody production and recurrent bacterial infections in addition abnormalities in T cells. CD4(+)CD25(high) regulatory T cells (Treg) are essential modulators of immune responses, including down-modulation of immune response to pathogens, allergens, cancer cells and self-antigens. OBJECTIVE: In this study we set out to investigate the frequency of Treg cells in CVID patients and correlate with their immune activation status. MATERIALS AND METHODS: Sixteen patients (6 males and 10 females) with CVID who had been treated with regular intravenous immunoglobulin and 14 controls were enrolled. Quantitative analyses of peripheral blood mononuclear cells (PBMC) were performed by multiparametric flow cytometry using the following cell markers: CD38, HLA-DR, CCR5 (immune activation); CD4, CD25, FOXP3, CD127, and OX40 (Treg cells); Ki-67 and IFN-gamma (intracellular cytokine). RESULTS: A significantly lower proportion of CD4(+)CD25(high)FOXP3 T cells was observed in CVID patients compared with healthy controls (P<0.05). In addition to a higher proportion of CD8(+) T cells from CVID patients expressing the activation markers, CD38(+) and HLA-DR(+) (P<0.05), we observed no significant correlation between Tregs and immune activation. CONCLUSION: Our results demonstrate that a reduction in Treg cells could have impaired immune function in CVID patients. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/19649263/A_decreased_frequency_of_regulatory_T_cells_in_patients_with_common_variable_immunodeficiency_ L2 - http://dx.plos.org/10.1371/journal.pone.0006269 DB - PRIME DP - Unbound Medicine ER -