Tags

Type your tag names separated by a space and hit enter

Activation of nanoparticles by biosorption for E. coli detection in milk and apple juice.
Appl Biochem Biotechnol. 2010 Sep; 162(2):460-75.AB

Abstract

Two types of silver nanoparticles were activated by specific sorption of biomolecules for the detection of Escherichia coli. The capture of this bacterium was performed using polyclonal antibodies (anti-E. coli) biosorbed onto nanospheres or nanorice through a protein-A layer. The bacterial detection was achieved using surface enhancement Raman scattering in order to compare the performance of these two nanoparticles. The activated silver nanospheres showed a better performance mainly due to the dimension of these nanoparticles. The detection limit has been established using the automated Raman mapping system. The technique was capable of detecting 10(3) cells/mL in milk and apple juice without any pre-enrichment. With an overall assay time less than 1 h, the process could be easily adapted to detect other pathogens by selecting the pertinent antibody. Furthermore, PCR was used for the DNA verification to assess whether the selected bacterial strain was identical before and after detection.

Authors+Show Affiliations

Biotechnology Research Institute, National Research Council Canada, H4P 2R2 Montreal, Quebec, Canada. mnaja@evergladesfoundation.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19649746

Citation

Naja, Ghinwa, et al. "Activation of Nanoparticles By Biosorption for E. Coli Detection in Milk and Apple Juice." Applied Biochemistry and Biotechnology, vol. 162, no. 2, 2010, pp. 460-75.
Naja G, Bouvrette P, Champagne J, et al. Activation of nanoparticles by biosorption for E. coli detection in milk and apple juice. Appl Biochem Biotechnol. 2010;162(2):460-75.
Naja, G., Bouvrette, P., Champagne, J., Brousseau, R., & Luong, J. H. (2010). Activation of nanoparticles by biosorption for E. coli detection in milk and apple juice. Applied Biochemistry and Biotechnology, 162(2), 460-75. https://doi.org/10.1007/s12010-009-8709-6
Naja G, et al. Activation of Nanoparticles By Biosorption for E. Coli Detection in Milk and Apple Juice. Appl Biochem Biotechnol. 2010;162(2):460-75. PubMed PMID: 19649746.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of nanoparticles by biosorption for E. coli detection in milk and apple juice. AU - Naja,Ghinwa, AU - Bouvrette,Pierre, AU - Champagne,Julie, AU - Brousseau,Roland, AU - Luong,John H T, Y1 - 2009/08/01/ PY - 2009/06/08/received PY - 2009/07/05/accepted PY - 2009/8/4/entrez PY - 2009/8/4/pubmed PY - 2010/7/31/medline SP - 460 EP - 75 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 162 IS - 2 N2 - Two types of silver nanoparticles were activated by specific sorption of biomolecules for the detection of Escherichia coli. The capture of this bacterium was performed using polyclonal antibodies (anti-E. coli) biosorbed onto nanospheres or nanorice through a protein-A layer. The bacterial detection was achieved using surface enhancement Raman scattering in order to compare the performance of these two nanoparticles. The activated silver nanospheres showed a better performance mainly due to the dimension of these nanoparticles. The detection limit has been established using the automated Raman mapping system. The technique was capable of detecting 10(3) cells/mL in milk and apple juice without any pre-enrichment. With an overall assay time less than 1 h, the process could be easily adapted to detect other pathogens by selecting the pertinent antibody. Furthermore, PCR was used for the DNA verification to assess whether the selected bacterial strain was identical before and after detection. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/19649746/Activation_of_nanoparticles_by_biosorption_for_E__coli_detection_in_milk_and_apple_juice_ L2 - https://dx.doi.org/10.1007/s12010-009-8709-6 DB - PRIME DP - Unbound Medicine ER -