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Overexpression of the lily p70(s6k) gene in Arabidopsis affects elongation of flower organs and indicates TOR-dependent regulation of AP3, PI and SUP translation.
Plant Cell Physiol. 2009 Sep; 50(9):1695-709.PC

Abstract

The p70 ribosomal S6 kinase (p70(s6k)) signaling pathway plays a key role in regulating the cell cycle via translational regulation of specific 5'TOP mRNAs. However, the function of this signaling pathway is still poorly understood in plants. Ectopic expression of the lily putative p70(s6k) gene, LS6K1, resulted in up-regulation of NAP (NAC-LIKE, ACTIVATED BY AP3/PI) and PISTILLATA (PI) expression, and significantly inhibited cell expansion for petals and stamens, resulting in the male sterility phenotype in transgenic Arabidopsis. Sequence analysis revealed that the genes involved in petal and stamen development, such as APETALA3 (AP3), PI and SUPERMAN (SUP), probably encode 5'TOP mRNAs. Green fluorescent protein (GFP), fused to oligopyrimidine tract sequences that were identified in the 5'-untranslated region (UTR) of AP3, PI and SUP, was translationally regulated in human cells in response to mitogen stimulation and inhibition by the macrolide antibiotic rapamycin. Furthermore, 35S::LS6K1 significantly up-regulated beta-glucuronidase (GUS) activity in the flower buds of transgenic plants carrying the GUS transgene fused to the AP3 promoter and the 5' UTR. These results have identified a novel role for the p70(s6k) gene in regulating cell division and the expansion of petals and stamens by translational regulation of the 5'TOP mRNAs once ectopically expressed in Arabidopsis.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19651701

Citation

Tzeng, Tsai-Yu, et al. "Overexpression of the Lily P70(s6k) Gene in Arabidopsis Affects Elongation of Flower Organs and Indicates TOR-dependent Regulation of AP3, PI and SUP Translation." Plant & Cell Physiology, vol. 50, no. 9, 2009, pp. 1695-709.
Tzeng TY, Kong LR, Chen CH, et al. Overexpression of the lily p70(s6k) gene in Arabidopsis affects elongation of flower organs and indicates TOR-dependent regulation of AP3, PI and SUP translation. Plant Cell Physiol. 2009;50(9):1695-709.
Tzeng, T. Y., Kong, L. R., Chen, C. H., Shaw, C. C., & Yang, C. H. (2009). Overexpression of the lily p70(s6k) gene in Arabidopsis affects elongation of flower organs and indicates TOR-dependent regulation of AP3, PI and SUP translation. Plant & Cell Physiology, 50(9), 1695-709. https://doi.org/10.1093/pcp/pcp114
Tzeng TY, et al. Overexpression of the Lily P70(s6k) Gene in Arabidopsis Affects Elongation of Flower Organs and Indicates TOR-dependent Regulation of AP3, PI and SUP Translation. Plant Cell Physiol. 2009;50(9):1695-709. PubMed PMID: 19651701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of the lily p70(s6k) gene in Arabidopsis affects elongation of flower organs and indicates TOR-dependent regulation of AP3, PI and SUP translation. AU - Tzeng,Tsai-Yu, AU - Kong,Lih-Ren, AU - Chen,Chun-Hung, AU - Shaw,Chih-Chi, AU - Yang,Chang-Hsien, Y1 - 2009/08/03/ PY - 2009/8/5/entrez PY - 2009/8/5/pubmed PY - 2009/10/17/medline SP - 1695 EP - 709 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 50 IS - 9 N2 - The p70 ribosomal S6 kinase (p70(s6k)) signaling pathway plays a key role in regulating the cell cycle via translational regulation of specific 5'TOP mRNAs. However, the function of this signaling pathway is still poorly understood in plants. Ectopic expression of the lily putative p70(s6k) gene, LS6K1, resulted in up-regulation of NAP (NAC-LIKE, ACTIVATED BY AP3/PI) and PISTILLATA (PI) expression, and significantly inhibited cell expansion for petals and stamens, resulting in the male sterility phenotype in transgenic Arabidopsis. Sequence analysis revealed that the genes involved in petal and stamen development, such as APETALA3 (AP3), PI and SUPERMAN (SUP), probably encode 5'TOP mRNAs. Green fluorescent protein (GFP), fused to oligopyrimidine tract sequences that were identified in the 5'-untranslated region (UTR) of AP3, PI and SUP, was translationally regulated in human cells in response to mitogen stimulation and inhibition by the macrolide antibiotic rapamycin. Furthermore, 35S::LS6K1 significantly up-regulated beta-glucuronidase (GUS) activity in the flower buds of transgenic plants carrying the GUS transgene fused to the AP3 promoter and the 5' UTR. These results have identified a novel role for the p70(s6k) gene in regulating cell division and the expansion of petals and stamens by translational regulation of the 5'TOP mRNAs once ectopically expressed in Arabidopsis. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/19651701/Overexpression_of_the_lily_p70_s6k__gene_in_Arabidopsis_affects_elongation_of_flower_organs_and_indicates_TOR_dependent_regulation_of_AP3_PI_and_SUP_translation_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcp114 DB - PRIME DP - Unbound Medicine ER -