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A fragmentation study of dihydroquercetin using triple quadrupole mass spectrometry and its application for identification of dihydroflavonols in Citrus juices.
Rapid Commun Mass Spectrom. 2009 Sep; 23(17):2785-92.RC

Abstract

A mass spectrometric method using electrospray ionization with triple quadrupole and quadrupole time-of-flight hybrid (Q-Tof) mass spectrometry has been applied to the structural characterization of dihydroflavonols. This family of compounds has been studied by liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the first time in this work. A comprehensive study of the product ion MS spectra of the [M+H](+) ion of a commercially available standard has been performed. The most useful fragmentations in terms of structural identification are those that involve cleavage of the C-ring, resulting in diagnostic ions of dihydroflavonol family: (1,3)A(0) (+), (1,2)B(0) (+), (1,2)B(0) (+)-CO, (0,2)A(0) (+), (0,2)A(0) (+)-H(2)O, (0,2)A(0) (+)-CO, and (0,2)A(0) (+)-H(2)O-CO, that allow the characterization of the substituents in the A- and B-rings. In addition to those ions, other product ions due to losses of H(2)O and CO molecules from the Y(0) (+) ion were observed. Their fragmentation mechanisms and ion structures have been proposed. The established fragmentation patterns have been used to successfully identity three dihydroflavonols found in tangerine juices for the first time.

Authors+Show Affiliations

Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, P.O. Box 644, 48080 Bilbao, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19653204

Citation

Abad-García, Beatriz, et al. "A Fragmentation Study of Dihydroquercetin Using Triple Quadrupole Mass Spectrometry and Its Application for Identification of Dihydroflavonols in Citrus Juices." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 17, 2009, pp. 2785-92.
Abad-García B, Garmón-Lobato S, Berrueta LA, et al. A fragmentation study of dihydroquercetin using triple quadrupole mass spectrometry and its application for identification of dihydroflavonols in Citrus juices. Rapid Commun Mass Spectrom. 2009;23(17):2785-92.
Abad-García, B., Garmón-Lobato, S., Berrueta, L. A., Gallo, B., & Vicente, F. (2009). A fragmentation study of dihydroquercetin using triple quadrupole mass spectrometry and its application for identification of dihydroflavonols in Citrus juices. Rapid Communications in Mass Spectrometry : RCM, 23(17), 2785-92. https://doi.org/10.1002/rcm.4182
Abad-García B, et al. A Fragmentation Study of Dihydroquercetin Using Triple Quadrupole Mass Spectrometry and Its Application for Identification of Dihydroflavonols in Citrus Juices. Rapid Commun Mass Spectrom. 2009;23(17):2785-92. PubMed PMID: 19653204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fragmentation study of dihydroquercetin using triple quadrupole mass spectrometry and its application for identification of dihydroflavonols in Citrus juices. AU - Abad-García,Beatriz, AU - Garmón-Lobato,Sergio, AU - Berrueta,Luis A, AU - Gallo,Blanca, AU - Vicente,Francisca, PY - 2009/8/5/entrez PY - 2009/8/5/pubmed PY - 2009/10/8/medline SP - 2785 EP - 92 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 17 N2 - A mass spectrometric method using electrospray ionization with triple quadrupole and quadrupole time-of-flight hybrid (Q-Tof) mass spectrometry has been applied to the structural characterization of dihydroflavonols. This family of compounds has been studied by liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the first time in this work. A comprehensive study of the product ion MS spectra of the [M+H](+) ion of a commercially available standard has been performed. The most useful fragmentations in terms of structural identification are those that involve cleavage of the C-ring, resulting in diagnostic ions of dihydroflavonol family: (1,3)A(0) (+), (1,2)B(0) (+), (1,2)B(0) (+)-CO, (0,2)A(0) (+), (0,2)A(0) (+)-H(2)O, (0,2)A(0) (+)-CO, and (0,2)A(0) (+)-H(2)O-CO, that allow the characterization of the substituents in the A- and B-rings. In addition to those ions, other product ions due to losses of H(2)O and CO molecules from the Y(0) (+) ion were observed. Their fragmentation mechanisms and ion structures have been proposed. The established fragmentation patterns have been used to successfully identity three dihydroflavonols found in tangerine juices for the first time. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/19653204/A_fragmentation_study_of_dihydroquercetin_using_triple_quadrupole_mass_spectrometry_and_its_application_for_identification_of_dihydroflavonols_in_Citrus_juices_ L2 - https://doi.org/10.1002/rcm.4182 DB - PRIME DP - Unbound Medicine ER -