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Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples.
Electrophoresis. 2009 Aug; 30(16):2773-802.E

Abstract

An analysis of recent trends indicates that CE can show real advantages over chromatographic methods in ultratrace enantioselective determination of biologically active compounds in complex biological matrices. It is due to high separation efficiency and many applicable in-capillary electromigration effects in CE (countercurrent migration, stacking effects) enhancing significantly (enantio)separability and enabling effective sample preparation (preconcentration, purification, analyte derivatization). Other possible on-line combinations of CE, such as column coupled CE-CE techniques and implementation of nonelectrophoretic techniques (extraction, membrane filtration, flow injection) into CE, offer additional approaches for highly effective sample preparation and separation. CE matured to a highly flexible and compatible technique enabling its hyphenation with powerful detection systems allowing extremely sensitive detection (e.g. LIF) and/or structural characterization of analytes (e.g. MS). Within the last decade, more as well as less conventional analytical on-line approaches have been effectively utilized in this field and their practical potentialities are demonstrated on many new application examples in this article. Here, three basic areas of (enantioselective) drug bioanalysis are highlighted and supported by a brief theoretical description of each individual approach in a compact review structure (to create integrated view on the topic), including (i) progressive enantioseparation approaches and new enantioselective agents, (ii) in-capillary sample preparation (preconcentration, purification, derivatization), and (iii) detection possibilities related to enhanced sensitivity and structural characterization.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Mikus P
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic. mikus@fpharm.uniba.sk
Maráková K
No affiliation info available

MeSH

BiomarkersCapillary ElectrochromatographyCyclodextrinsElectrophoresis, CapillaryPharmaceutical PreparationsSpectrum AnalysisStereoisomerism

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19653234

Citation

Mikus, Peter, and Katarína Maráková. "Advanced CE for Chiral Analysis of Drugs, Metabolites, and Biomarkers in Biological Samples." Electrophoresis, vol. 30, no. 16, 2009, pp. 2773-802.
Mikus P, Maráková K. Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples. Electrophoresis. 2009;30(16):2773-802.
Mikus, P., & Maráková, K. (2009). Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples. Electrophoresis, 30(16), 2773-802. https://doi.org/10.1002/elps.200900173
Mikus P, Maráková K. Advanced CE for Chiral Analysis of Drugs, Metabolites, and Biomarkers in Biological Samples. Electrophoresis. 2009;30(16):2773-802. PubMed PMID: 19653234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples. AU - Mikus,Peter, AU - Maráková,Katarína, PY - 2009/8/5/entrez PY - 2009/8/5/pubmed PY - 2010/1/6/medline SP - 2773 EP - 802 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 16 N2 - An analysis of recent trends indicates that CE can show real advantages over chromatographic methods in ultratrace enantioselective determination of biologically active compounds in complex biological matrices. It is due to high separation efficiency and many applicable in-capillary electromigration effects in CE (countercurrent migration, stacking effects) enhancing significantly (enantio)separability and enabling effective sample preparation (preconcentration, purification, analyte derivatization). Other possible on-line combinations of CE, such as column coupled CE-CE techniques and implementation of nonelectrophoretic techniques (extraction, membrane filtration, flow injection) into CE, offer additional approaches for highly effective sample preparation and separation. CE matured to a highly flexible and compatible technique enabling its hyphenation with powerful detection systems allowing extremely sensitive detection (e.g. LIF) and/or structural characterization of analytes (e.g. MS). Within the last decade, more as well as less conventional analytical on-line approaches have been effectively utilized in this field and their practical potentialities are demonstrated on many new application examples in this article. Here, three basic areas of (enantioselective) drug bioanalysis are highlighted and supported by a brief theoretical description of each individual approach in a compact review structure (to create integrated view on the topic), including (i) progressive enantioseparation approaches and new enantioselective agents, (ii) in-capillary sample preparation (preconcentration, purification, derivatization), and (iii) detection possibilities related to enhanced sensitivity and structural characterization. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19653234/Advanced_CE_for_chiral_analysis_of_drugs_metabolites_and_biomarkers_in_biological_samples_ L2 - https://doi.org/10.1002/elps.200900173 DB - PRIME DP - Unbound Medicine ER -