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Effects of allopurinol on uric acid concentrations, xanthine oxidoreductase activity and oxidative stress in broiler chickens.

Abstract

The purpose of this study was to determine the effects of allopurinol (AL) on xanthine oxidoreductase (XOR) activity and uric acid (UA) levels in chickens. Thirty 5-week-old broilers were divided into three groups and fed 0 (control), 25 (AL25) or 50 (AL50) mg AL per kg of body mass for 5 weeks. Chicks were weighed twice weekly and leukocyte oxidative activity (LOA) and plasma purine levels were determined weekly in five birds per group. Chicks were sacrificed after 2 or 5 weeks, and samples from tissues were taken for analysis of XOR activity. Plasma UA concentrations were lower (P<0.001) and xanthine and hypoxanthine concentrations were greater (P<0.001) in AL25 and AL50 birds compared to controls, whereas no differences (P=0.904) were detected in allantoin concentrations. By week 5, body mass was reduced (P<0.001) to 84.0 and 65.1% of that in controls for AL25 and AL50 broilers, respectively, and LOA was 4.1 times greater (P<0.05) in AL25 compared to control birds. Liver XOR activity was increased by 1.1 and 1.2 times in AL25 and AL50 birds, but there was no change (P>0.05) in XOR activity in the pancreas and intestine. These results suggest that AL effect on XOR activity is tissue dependent.

Authors+Show Affiliations

Division of Animal & Nutritional Sciences, West Virginia University, Morgantown, WV 26506-6108, USA. mdcart@unileon.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19654053

Citation

Carro, M D., et al. "Effects of Allopurinol On Uric Acid Concentrations, Xanthine Oxidoreductase Activity and Oxidative Stress in Broiler Chickens." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 151, no. 1, 2010, pp. 12-7.
Carro MD, Falkenstein E, Radke WJ, et al. Effects of allopurinol on uric acid concentrations, xanthine oxidoreductase activity and oxidative stress in broiler chickens. Comp Biochem Physiol C Toxicol Pharmacol. 2010;151(1):12-7.
Carro, M. D., Falkenstein, E., Radke, W. J., & Klandorf, H. (2010). Effects of allopurinol on uric acid concentrations, xanthine oxidoreductase activity and oxidative stress in broiler chickens. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 151(1), 12-7. https://doi.org/10.1016/j.cbpc.2009.07.010
Carro MD, et al. Effects of Allopurinol On Uric Acid Concentrations, Xanthine Oxidoreductase Activity and Oxidative Stress in Broiler Chickens. Comp Biochem Physiol C Toxicol Pharmacol. 2010;151(1):12-7. PubMed PMID: 19654053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of allopurinol on uric acid concentrations, xanthine oxidoreductase activity and oxidative stress in broiler chickens. AU - Carro,M D, AU - Falkenstein,E, AU - Radke,W J, AU - Klandorf,H, Y1 - 2009/08/03/ PY - 2009/06/19/received PY - 2009/07/23/revised PY - 2009/07/24/accepted PY - 2009/8/6/entrez PY - 2009/8/6/pubmed PY - 2010/2/24/medline SP - 12 EP - 7 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 151 IS - 1 N2 - The purpose of this study was to determine the effects of allopurinol (AL) on xanthine oxidoreductase (XOR) activity and uric acid (UA) levels in chickens. Thirty 5-week-old broilers were divided into three groups and fed 0 (control), 25 (AL25) or 50 (AL50) mg AL per kg of body mass for 5 weeks. Chicks were weighed twice weekly and leukocyte oxidative activity (LOA) and plasma purine levels were determined weekly in five birds per group. Chicks were sacrificed after 2 or 5 weeks, and samples from tissues were taken for analysis of XOR activity. Plasma UA concentrations were lower (P<0.001) and xanthine and hypoxanthine concentrations were greater (P<0.001) in AL25 and AL50 birds compared to controls, whereas no differences (P=0.904) were detected in allantoin concentrations. By week 5, body mass was reduced (P<0.001) to 84.0 and 65.1% of that in controls for AL25 and AL50 broilers, respectively, and LOA was 4.1 times greater (P<0.05) in AL25 compared to control birds. Liver XOR activity was increased by 1.1 and 1.2 times in AL25 and AL50 birds, but there was no change (P>0.05) in XOR activity in the pancreas and intestine. These results suggest that AL effect on XOR activity is tissue dependent. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/19654053/Effects_of_allopurinol_on_uric_acid_concentrations_xanthine_oxidoreductase_activity_and_oxidative_stress_in_broiler_chickens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(09)00174-4 DB - PRIME DP - Unbound Medicine ER -