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Antimicrobial activity of gallotannins isolated from mango (Mangifera indica L.) kernels.
J Agric Food Chem. 2009 Sep 09; 57(17):7712-8.JA

Abstract

Gallotannins were extracted from mango (Mangifera indica L.) kernels with aqueous acetone (80%, v/v) and purified using liquid-liquid extraction and two-step low-pressure liquid chromatography (LPLC) on Sephadex LH-20. Analytical high-performance liquid chromatography and mass spectrometry confirmed the presence of hydrolyzable tannins with a degree of galloylation ranging from 4 to 9 and additionally revealed the presence of deca-, undeca-, and dodeca-O-galloylglucose. Further purification using two-step semipreparative HPLC resulted in three pure hydrolyzable tannins, penta-, hexa-, and hepta-O-galloylglucose, with antibacterial activity, as evidenced from the agar spot and critical dilution assays. Although the growth of lactic acid bacteria was not inhibited, the proliferation of Gram-positive food spoilage bacteria was prevented and the growth of Gram-negative Escherichia coli was reduced. Because bacterial growth could be restored by the addition of iron to the medium, this study strongly supports the view that the inhibitory effects of hydrolyzable tannins are due to their iron-complexing properties.

Authors+Show Affiliations

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19655802

Citation

Engels, Christina, et al. "Antimicrobial Activity of Gallotannins Isolated From Mango (Mangifera Indica L.) Kernels." Journal of Agricultural and Food Chemistry, vol. 57, no. 17, 2009, pp. 7712-8.
Engels C, Knödler M, Zhao YY, et al. Antimicrobial activity of gallotannins isolated from mango (Mangifera indica L.) kernels. J Agric Food Chem. 2009;57(17):7712-8.
Engels, C., Knödler, M., Zhao, Y. Y., Carle, R., Gänzle, M. G., & Schieber, A. (2009). Antimicrobial activity of gallotannins isolated from mango (Mangifera indica L.) kernels. Journal of Agricultural and Food Chemistry, 57(17), 7712-8. https://doi.org/10.1021/jf901621m
Engels C, et al. Antimicrobial Activity of Gallotannins Isolated From Mango (Mangifera Indica L.) Kernels. J Agric Food Chem. 2009 Sep 9;57(17):7712-8. PubMed PMID: 19655802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial activity of gallotannins isolated from mango (Mangifera indica L.) kernels. AU - Engels,Christina, AU - Knödler,Matthias, AU - Zhao,Yuan-Yuan, AU - Carle,Reinhold, AU - Gänzle,Michael G, AU - Schieber,Andreas, PY - 2009/8/7/entrez PY - 2009/8/7/pubmed PY - 2009/12/16/medline SP - 7712 EP - 8 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 17 N2 - Gallotannins were extracted from mango (Mangifera indica L.) kernels with aqueous acetone (80%, v/v) and purified using liquid-liquid extraction and two-step low-pressure liquid chromatography (LPLC) on Sephadex LH-20. Analytical high-performance liquid chromatography and mass spectrometry confirmed the presence of hydrolyzable tannins with a degree of galloylation ranging from 4 to 9 and additionally revealed the presence of deca-, undeca-, and dodeca-O-galloylglucose. Further purification using two-step semipreparative HPLC resulted in three pure hydrolyzable tannins, penta-, hexa-, and hepta-O-galloylglucose, with antibacterial activity, as evidenced from the agar spot and critical dilution assays. Although the growth of lactic acid bacteria was not inhibited, the proliferation of Gram-positive food spoilage bacteria was prevented and the growth of Gram-negative Escherichia coli was reduced. Because bacterial growth could be restored by the addition of iron to the medium, this study strongly supports the view that the inhibitory effects of hydrolyzable tannins are due to their iron-complexing properties. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19655802/Antimicrobial_activity_of_gallotannins_isolated_from_mango__Mangifera_indica_L___kernels_ L2 - https://doi.org/10.1021/jf901621m DB - PRIME DP - Unbound Medicine ER -