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Immunohistochemical study of HLA-G expression in lung transplant recipients.
Am J Transplant. 2009 Jun; 9(6):1427-38.AJ

Abstract

Human leukocyte antigen-G (HLA-G), a nonclassical HLA class I protein, promotes immune tolerance of solid-organ allografts, yet its role in lung transplantation (LTx) is unknown. We examined the expression of HLA-G in lung allografts through immunohistochemistry by a cross-sectional study of 64 LTx recipients, classified into four groups (stable patients, acute rejection [AR], bronchiolitis obliterans syndrome [BOS] and symptomatic viral shedders). A marked expression of HLA-G in bronchial epithelial cells (BEC) was frequently observed in stable recipients (n = 18/35 [51%]), but not in patients with AR (n = 14) or with BOS (n = 8). HLA-G was also expressed by 4 of 7 symptomatic viral shedders. In addition, HLA-G-positive patients from the stable group (n = 35) experienced lower incidence of resistant AR and/or BOS during long-term follow-up, as compared with their HLA-G-negative counterparts. Finally, in vitro data showed that interferon-gamma, a cytokine present in lung allograft microenvironment, upregulated HLA-G mRNA and protein expression in primary cultured human BEC. We conclude that HLA-G expression in the bronchial epithelium of lung allograft is elevated in some LTx recipients in association with their functional stability, suggesting a potential role of HLA-G as a tolerance marker.

Authors+Show Affiliations

Service de Pneumologie B et de Transplantation Pulmonaire, Centre Hospitalier Universitaire Bichat-Claude Bernard, Paris, France. olivier.brugiere@bch.aphp.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19656137

Citation

Brugière, O, et al. "Immunohistochemical Study of HLA-G Expression in Lung Transplant Recipients." American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, vol. 9, no. 6, 2009, pp. 1427-38.
Brugière O, Thabut G, Pretolani M, et al. Immunohistochemical study of HLA-G expression in lung transplant recipients. Am J Transplant. 2009;9(6):1427-38.
Brugière, O., Thabut, G., Pretolani, M., Krawice-Radanne, I., Dill, C., Herbreteau, A., Poras, I., Moreau, P., Colombat, M., Danel, C., Dehoux, M., Fournier, M., Carosella, E. D., & Rouas-Freiss, N. (2009). Immunohistochemical study of HLA-G expression in lung transplant recipients. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 9(6), 1427-38. https://doi.org/10.1111/j.1600-6143.2009.02650.x
Brugière O, et al. Immunohistochemical Study of HLA-G Expression in Lung Transplant Recipients. Am J Transplant. 2009;9(6):1427-38. PubMed PMID: 19656137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical study of HLA-G expression in lung transplant recipients. AU - Brugière,O, AU - Thabut,G, AU - Pretolani,M, AU - Krawice-Radanne,I, AU - Dill,C, AU - Herbreteau,A, AU - Poras,I, AU - Moreau,P, AU - Colombat,M, AU - Danel,C, AU - Dehoux,M, AU - Fournier,M, AU - Carosella,E D, AU - Rouas-Freiss,N, PY - 2009/8/7/entrez PY - 2009/8/7/pubmed PY - 2009/12/16/medline SP - 1427 EP - 38 JF - American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons JO - Am. J. Transplant. VL - 9 IS - 6 N2 - Human leukocyte antigen-G (HLA-G), a nonclassical HLA class I protein, promotes immune tolerance of solid-organ allografts, yet its role in lung transplantation (LTx) is unknown. We examined the expression of HLA-G in lung allografts through immunohistochemistry by a cross-sectional study of 64 LTx recipients, classified into four groups (stable patients, acute rejection [AR], bronchiolitis obliterans syndrome [BOS] and symptomatic viral shedders). A marked expression of HLA-G in bronchial epithelial cells (BEC) was frequently observed in stable recipients (n = 18/35 [51%]), but not in patients with AR (n = 14) or with BOS (n = 8). HLA-G was also expressed by 4 of 7 symptomatic viral shedders. In addition, HLA-G-positive patients from the stable group (n = 35) experienced lower incidence of resistant AR and/or BOS during long-term follow-up, as compared with their HLA-G-negative counterparts. Finally, in vitro data showed that interferon-gamma, a cytokine present in lung allograft microenvironment, upregulated HLA-G mRNA and protein expression in primary cultured human BEC. We conclude that HLA-G expression in the bronchial epithelium of lung allograft is elevated in some LTx recipients in association with their functional stability, suggesting a potential role of HLA-G as a tolerance marker. SN - 1600-6143 UR - https://www.unboundmedicine.com/medline/citation/19656137/Immunohistochemical_study_of_HLA_G_expression_in_lung_transplant_recipients_ L2 - https://doi.org/10.1111/j.1600-6143.2009.02650.x DB - PRIME DP - Unbound Medicine ER -