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Novel cyanophage photosynthetic gene psbA in the floodwater of a Japanese rice field.FEMS Microbiol Ecol. 2009 Oct; 70(1):79-86.FM
Abstract
The gene psbA, encoding the D1 protein involved in photosynthesis, was recently found in a number of cultured cyanophages infecting marine Synechococcus and Prochlorococcus and in environmental samples from marine and freshwaters. In this study, viral concentrates were prepared by sampling the floodwaters from each of four plots in a Japanese rice field: (1) no fertilizer; (2) P and K chemical fertilizers; (3) N, P and K chemical fertilizers; and (4) chemical fertilizers with compost. Fragments of the cyanophage psbA gene were amplified by PCR from DNA in the viral concentrates, with primers psbA-F and psbA-R. Double denaturing gradient gel electrophoresis was conducted to obtain different psbA clones. Phylogenetic analyses indicated that the majority of the psbA sequences in the floodwater formed two unique groups, with their sequences being more closely related to those from freshwater samples than the sequences obtained from marine waters, suggesting that psbA genes in terrestrial aquatic environments are different from those in marine environments.
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MeSH
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
19659578
Citation
Wang, Guanghua, et al. "Novel Cyanophage Photosynthetic Gene psbA in the Floodwater of a Japanese Rice Field." FEMS Microbiology Ecology, vol. 70, no. 1, 2009, pp. 79-86.
Wang G, Murase J, Asakawa S, et al. Novel cyanophage photosynthetic gene psbA in the floodwater of a Japanese rice field. FEMS Microbiol Ecol. 2009;70(1):79-86.
Wang, G., Murase, J., Asakawa, S., & Kimura, M. (2009). Novel cyanophage photosynthetic gene psbA in the floodwater of a Japanese rice field. FEMS Microbiology Ecology, 70(1), 79-86. https://doi.org/10.1111/j.1574-6941.2009.00743.x
Wang G, et al. Novel Cyanophage Photosynthetic Gene psbA in the Floodwater of a Japanese Rice Field. FEMS Microbiol Ecol. 2009;70(1):79-86. PubMed PMID: 19659578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Novel cyanophage photosynthetic gene psbA in the floodwater of a Japanese rice field.
AU - Wang,Guanghua,
AU - Murase,Jun,
AU - Asakawa,Susumu,
AU - Kimura,Makoto,
Y1 - 2009/07/10/
PY - 2009/8/8/entrez
PY - 2009/8/8/pubmed
PY - 2009/10/20/medline
SP - 79
EP - 86
JF - FEMS microbiology ecology
JO - FEMS Microbiol Ecol
VL - 70
IS - 1
N2 - The gene psbA, encoding the D1 protein involved in photosynthesis, was recently found in a number of cultured cyanophages infecting marine Synechococcus and Prochlorococcus and in environmental samples from marine and freshwaters. In this study, viral concentrates were prepared by sampling the floodwaters from each of four plots in a Japanese rice field: (1) no fertilizer; (2) P and K chemical fertilizers; (3) N, P and K chemical fertilizers; and (4) chemical fertilizers with compost. Fragments of the cyanophage psbA gene were amplified by PCR from DNA in the viral concentrates, with primers psbA-F and psbA-R. Double denaturing gradient gel electrophoresis was conducted to obtain different psbA clones. Phylogenetic analyses indicated that the majority of the psbA sequences in the floodwater formed two unique groups, with their sequences being more closely related to those from freshwater samples than the sequences obtained from marine waters, suggesting that psbA genes in terrestrial aquatic environments are different from those in marine environments.
SN - 1574-6941
UR - https://www.unboundmedicine.com/medline/citation/19659578/Novel_cyanophage_photosynthetic_gene_psbA_in_the_floodwater_of_a_Japanese_rice_field_
L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2009.00743.x
DB - PRIME
DP - Unbound Medicine
ER -
