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N9L and L9N mutations toggle Hha binding and hemolysin regulation by Escherichia coli and Vibrio cholerae H-NS.
FEBS Lett 2009; 583(17):2911-6FL

Abstract

Proteins of the Hha/YmoA family co-regulate with H-NS the expression of virulence factors in Enterobacteriaceae. Vibrio cholerae lacks Hha-like proteins and its H-NS (vcH-NS) is unable to bind Hha, in spite of the conservation of a key residue for Hha binding by Escherichia coli H-NS (ecH-NS). Exchange of the residues in position 9 between vcH-NS and ecH-NS strongly reduces Hha binding by ecH-NS and introduces it in vcH-NS. These mutations strongly affect the repression of the hemolysin operon in E. coli and the electrophoretic mobility of complexes formed with a DNA fragment containing its regulatory region.

Authors+Show Affiliations

Institute for Research in Biomedicine, Parc Científic de Barcelona, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19660457

Citation

García, Jesús, et al. "N9L and L9N Mutations Toggle Hha Binding and Hemolysin Regulation By Escherichia Coli and Vibrio Cholerae H-NS." FEBS Letters, vol. 583, no. 17, 2009, pp. 2911-6.
García J, Madrid C, Cendra M, et al. N9L and L9N mutations toggle Hha binding and hemolysin regulation by Escherichia coli and Vibrio cholerae H-NS. FEBS Lett. 2009;583(17):2911-6.
García, J., Madrid, C., Cendra, M., Juárez, A., & Pons, M. (2009). N9L and L9N mutations toggle Hha binding and hemolysin regulation by Escherichia coli and Vibrio cholerae H-NS. FEBS Letters, 583(17), pp. 2911-6. doi:10.1016/j.febslet.2009.07.054.
García J, et al. N9L and L9N Mutations Toggle Hha Binding and Hemolysin Regulation By Escherichia Coli and Vibrio Cholerae H-NS. FEBS Lett. 2009 Sep 3;583(17):2911-6. PubMed PMID: 19660457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N9L and L9N mutations toggle Hha binding and hemolysin regulation by Escherichia coli and Vibrio cholerae H-NS. AU - García,Jesús, AU - Madrid,Cristina, AU - Cendra,Mar, AU - Juárez,Antonio, AU - Pons,Miquel, Y1 - 2009/08/04/ PY - 2009/06/03/received PY - 2009/07/28/revised PY - 2009/07/29/accepted PY - 2009/8/8/entrez PY - 2009/8/8/pubmed PY - 2009/10/23/medline SP - 2911 EP - 6 JF - FEBS letters JO - FEBS Lett. VL - 583 IS - 17 N2 - Proteins of the Hha/YmoA family co-regulate with H-NS the expression of virulence factors in Enterobacteriaceae. Vibrio cholerae lacks Hha-like proteins and its H-NS (vcH-NS) is unable to bind Hha, in spite of the conservation of a key residue for Hha binding by Escherichia coli H-NS (ecH-NS). Exchange of the residues in position 9 between vcH-NS and ecH-NS strongly reduces Hha binding by ecH-NS and introduces it in vcH-NS. These mutations strongly affect the repression of the hemolysin operon in E. coli and the electrophoretic mobility of complexes formed with a DNA fragment containing its regulatory region. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/19660457/N9L_and_L9N_mutations_toggle_Hha_binding_and_hemolysin_regulation_by_Escherichia_coli_and_Vibrio_cholerae_H_NS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(09)00606-1 DB - PRIME DP - Unbound Medicine ER -