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Aminoglycoside-3''-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum.
Plant Mol Biol 1990; 14(2):197-205PM

Abstract

The bacterial gene aadA encodes the enzyme aminoglycoside-3"-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aadA gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.

Authors+Show Affiliations

DNA Plant Technology Corporation, Oakland, CA 94608.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1966273

Citation

Svab, Z, et al. "Aminoglycoside-3''-adenyltransferase Confers Resistance to Spectinomycin and Streptomycin in Nicotiana Tabacum." Plant Molecular Biology, vol. 14, no. 2, 1990, pp. 197-205.
Svab Z, Harper EC, Jones JD, et al. Aminoglycoside-3''-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum. Plant Mol Biol. 1990;14(2):197-205.
Svab, Z., Harper, E. C., Jones, J. D., & Maliga, P. (1990). Aminoglycoside-3''-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum. Plant Molecular Biology, 14(2), pp. 197-205.
Svab Z, et al. Aminoglycoside-3''-adenyltransferase Confers Resistance to Spectinomycin and Streptomycin in Nicotiana Tabacum. Plant Mol Biol. 1990;14(2):197-205. PubMed PMID: 1966273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aminoglycoside-3''-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum. AU - Svab,Z, AU - Harper,E C, AU - Jones,J D, AU - Maliga,P, PY - 1990/2/1/pubmed PY - 1990/2/1/medline PY - 1990/2/1/entrez SP - 197 EP - 205 JF - Plant molecular biology JO - Plant Mol. Biol. VL - 14 IS - 2 N2 - The bacterial gene aadA encodes the enzyme aminoglycoside-3"-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aadA gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin. SN - 0167-4412 UR - https://www.unboundmedicine.com/medline/citation/1966273/Aminoglycoside_3''_adenyltransferase_confers_resistance_to_spectinomycin_and_streptomycin_in_Nicotiana_tabacum_ L2 - https://medlineplus.gov/antibioticresistance.html DB - PRIME DP - Unbound Medicine ER -