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Adipocyte differentiation is inhibited by melatonin through the regulation of C/EBPbeta transcriptional activity.
J Pineal Res. 2009 Oct; 47(3):221-7.JP

Abstract

Considering that melatonin has been implicated in body weight control, this work investigated whether this effect involves the regulation of adipogenesis. 3T3-L1 preadipocytes were induced to differentiate in the absence or presence of melatonin (10(-3) m). Swiss-3T3 cells ectopically and conditionally (Tet-off system) over-expressing the 34 kDa C/EBPbeta isoform (Swiss-LAP cells) were employed as a tool to assess the mechanisms of action at the molecular level. Protein markers of the adipogenic phenotype were analyzed by Western blot. At 36 hr of differentiation of 3T3-L1 preadipocytes, a reduction of PPARgamma expression was detected followed by a further reduction, at day 4, of perilipin, aP2 and adiponectin protein expression in melatonin-treated cells. Real-time PCR analysis also showed a decrease of PPARgamma (60%), C/EBPalpha (75%), adiponectin (30%) and aP2 (40%) mRNA expression. Finally, we transfected Swiss LAP cells with a C/EBPalpha gene promoter/reporter construct in which luciferase expression is enhanced in response to C/EBPbeta activity. Culture of such transfected cells in the absence of tetracycline led to a 2.5-fold activation of the C/EBPalpha promoter. However, when treated with melatonin, the level of C/EBPalpha promoter activation by C/EBPbeta was reduced by 50% (P = 0.05, n = 6). In addition, this inhibitory effect of melatonin was also reflected in the phenotype of the cells, since their capacity to accumulate lipids droplets was reduced as confirmed by the poor staining with Oil Red O. In conclusion, melatonin at a concentration of 10(-3) m works as a negative regulator of adipogenesis acting in part by inhibiting the activity of a critical adipogenic transcription factor, C/EBPbeta.

Authors+Show Affiliations

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19663997

Citation

Alonso-Vale, Maria Isabel Cardoso, et al. "Adipocyte Differentiation Is Inhibited By Melatonin Through the Regulation of C/EBPbeta Transcriptional Activity." Journal of Pineal Research, vol. 47, no. 3, 2009, pp. 221-7.
Alonso-Vale MI, Peres SB, Vernochet C, et al. Adipocyte differentiation is inhibited by melatonin through the regulation of C/EBPbeta transcriptional activity. J Pineal Res. 2009;47(3):221-7.
Alonso-Vale, M. I., Peres, S. B., Vernochet, C., Farmer, S. R., & Lima, F. B. (2009). Adipocyte differentiation is inhibited by melatonin through the regulation of C/EBPbeta transcriptional activity. Journal of Pineal Research, 47(3), 221-7. https://doi.org/10.1111/j.1600-079X.2009.00705.x
Alonso-Vale MI, et al. Adipocyte Differentiation Is Inhibited By Melatonin Through the Regulation of C/EBPbeta Transcriptional Activity. J Pineal Res. 2009;47(3):221-7. PubMed PMID: 19663997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adipocyte differentiation is inhibited by melatonin through the regulation of C/EBPbeta transcriptional activity. AU - Alonso-Vale,Maria Isabel Cardoso, AU - Peres,Sidney Barnabé, AU - Vernochet,Cécile, AU - Farmer,Stephen R, AU - Lima,Fabio Bessa, Y1 - 2009/08/03/ PY - 2009/8/12/entrez PY - 2009/8/12/pubmed PY - 2010/1/13/medline SP - 221 EP - 7 JF - Journal of pineal research JO - J Pineal Res VL - 47 IS - 3 N2 - Considering that melatonin has been implicated in body weight control, this work investigated whether this effect involves the regulation of adipogenesis. 3T3-L1 preadipocytes were induced to differentiate in the absence or presence of melatonin (10(-3) m). Swiss-3T3 cells ectopically and conditionally (Tet-off system) over-expressing the 34 kDa C/EBPbeta isoform (Swiss-LAP cells) were employed as a tool to assess the mechanisms of action at the molecular level. Protein markers of the adipogenic phenotype were analyzed by Western blot. At 36 hr of differentiation of 3T3-L1 preadipocytes, a reduction of PPARgamma expression was detected followed by a further reduction, at day 4, of perilipin, aP2 and adiponectin protein expression in melatonin-treated cells. Real-time PCR analysis also showed a decrease of PPARgamma (60%), C/EBPalpha (75%), adiponectin (30%) and aP2 (40%) mRNA expression. Finally, we transfected Swiss LAP cells with a C/EBPalpha gene promoter/reporter construct in which luciferase expression is enhanced in response to C/EBPbeta activity. Culture of such transfected cells in the absence of tetracycline led to a 2.5-fold activation of the C/EBPalpha promoter. However, when treated with melatonin, the level of C/EBPalpha promoter activation by C/EBPbeta was reduced by 50% (P = 0.05, n = 6). In addition, this inhibitory effect of melatonin was also reflected in the phenotype of the cells, since their capacity to accumulate lipids droplets was reduced as confirmed by the poor staining with Oil Red O. In conclusion, melatonin at a concentration of 10(-3) m works as a negative regulator of adipogenesis acting in part by inhibiting the activity of a critical adipogenic transcription factor, C/EBPbeta. SN - 1600-079X UR - https://www.unboundmedicine.com/medline/citation/19663997/Adipocyte_differentiation_is_inhibited_by_melatonin_through_the_regulation_of_C/EBPbeta_transcriptional_activity_ L2 - https://doi.org/10.1111/j.1600-079X.2009.00705.x DB - PRIME DP - Unbound Medicine ER -