Tags

Type your tag names separated by a space and hit enter

Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism.
J Proteomics. 2009 Nov 02; 73(1):57-78.JP

Abstract

The venom proteomes of Bothrops atrox from Colombia, Brazil, Ecuador, and Perú were characterized using venomic and antivenomic strategies. Our results evidence the existence of two geographically differentiated venom phenotypes. The venom from Colombia comprises at least 26 different proteins belonging to 9 different groups of toxins. PI-metalloproteinases and K49-PLA(2) molecules represent the most abundant toxins. On the other hand, the venoms from Brazilian, Ecuadorian, and Peruvian B. atrox contain predominantly PIII-metalloproteinases. These toxin profiles correlate with the venom phenotypes of adult and juvenile B. asper from Costa Rica, respectively, suggesting that paedomorphism represented a selective trend during the trans-Amazonian southward expansion of B. atrox through the Andean Corridor. The high degree of crossreactivity of a Costa Rican polyvalent (Bothrops asper, Lachesis stenophrys, Crotalus simus) antivenom against B. atrox venoms further evidenced the close evolutionary kinship between B. asper and B. atrox. This antivenom was more efficient immunodepleting proteins from the venoms of B. atrox from Brazil, Ecuador, and Perú than from Colombia. Such behaviour may be rationalized taking into account the lower content of poorly immunogenic toxins, such as PLA(2) molecules and PI-SVMPs in the paedomorphic venoms. The immunological profile of the Costa Rican antivenom strongly suggests the possibility of using this antivenom for the management of snakebites by B. atrox in Colombia and the Amazon regions of Ecuador, Perú and Brazil.

Authors+Show Affiliations

Programa de Ofidismo/Escorpionismo, Universidad de Antioquia, Medellín 1226, Colombia; Escuela de Microbiología, Universidad de Antioquia, Medellín 1226, Colombia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19665598

Citation

Núñez, Vitelbina, et al. "Snake Venomics and Antivenomics of Bothrops Atrox Venoms From Colombia and the Amazon Regions of Brazil, Perú and Ecuador Suggest the Occurrence of Geographic Variation of Venom Phenotype By a Trend Towards Paedomorphism." Journal of Proteomics, vol. 73, no. 1, 2009, pp. 57-78.
Núñez V, Cid P, Sanz L, et al. Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism. J Proteomics. 2009;73(1):57-78.
Núñez, V., Cid, P., Sanz, L., De La Torre, P., Angulo, Y., Lomonte, B., Gutiérrez, J. M., & Calvete, J. J. (2009). Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism. Journal of Proteomics, 73(1), 57-78. https://doi.org/10.1016/j.jprot.2009.07.013
Núñez V, et al. Snake Venomics and Antivenomics of Bothrops Atrox Venoms From Colombia and the Amazon Regions of Brazil, Perú and Ecuador Suggest the Occurrence of Geographic Variation of Venom Phenotype By a Trend Towards Paedomorphism. J Proteomics. 2009 Nov 2;73(1):57-78. PubMed PMID: 19665598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism. AU - Núñez,Vitelbina, AU - Cid,Pedro, AU - Sanz,Libia, AU - De La Torre,Pilar, AU - Angulo,Yamileth, AU - Lomonte,Bruno, AU - Gutiérrez,José María, AU - Calvete,Juan J, Y1 - 2009/08/06/ PY - 2009/06/11/received PY - 2009/07/29/revised PY - 2009/07/29/accepted PY - 2009/8/12/entrez PY - 2009/8/12/pubmed PY - 2010/1/7/medline SP - 57 EP - 78 JF - Journal of proteomics JO - J Proteomics VL - 73 IS - 1 N2 - The venom proteomes of Bothrops atrox from Colombia, Brazil, Ecuador, and Perú were characterized using venomic and antivenomic strategies. Our results evidence the existence of two geographically differentiated venom phenotypes. The venom from Colombia comprises at least 26 different proteins belonging to 9 different groups of toxins. PI-metalloproteinases and K49-PLA(2) molecules represent the most abundant toxins. On the other hand, the venoms from Brazilian, Ecuadorian, and Peruvian B. atrox contain predominantly PIII-metalloproteinases. These toxin profiles correlate with the venom phenotypes of adult and juvenile B. asper from Costa Rica, respectively, suggesting that paedomorphism represented a selective trend during the trans-Amazonian southward expansion of B. atrox through the Andean Corridor. The high degree of crossreactivity of a Costa Rican polyvalent (Bothrops asper, Lachesis stenophrys, Crotalus simus) antivenom against B. atrox venoms further evidenced the close evolutionary kinship between B. asper and B. atrox. This antivenom was more efficient immunodepleting proteins from the venoms of B. atrox from Brazil, Ecuador, and Perú than from Colombia. Such behaviour may be rationalized taking into account the lower content of poorly immunogenic toxins, such as PLA(2) molecules and PI-SVMPs in the paedomorphic venoms. The immunological profile of the Costa Rican antivenom strongly suggests the possibility of using this antivenom for the management of snakebites by B. atrox in Colombia and the Amazon regions of Ecuador, Perú and Brazil. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/19665598/Snake_venomics_and_antivenomics_of_Bothrops_atrox_venoms_from_Colombia_and_the_Amazon_regions_of_Brazil_Perú_and_Ecuador_suggest_the_occurrence_of_geographic_variation_of_venom_phenotype_by_a_trend_towards_paedomorphism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(09)00238-3 DB - PRIME DP - Unbound Medicine ER -