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Quaternary alkaloid, pseudocoptisine isolated from tubers of Corydalis turtschaninovi inhibits LPS-induced nitric oxide, PGE(2), and pro-inflammatory cytokines production via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells.
Int Immunopharmacol. 2009 Oct; 9(11):1323-31.II

Abstract

It is well known that pro-inflammatory mediators like nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6) contribute to the courses of many inflammatory diseases. In the present study, the authors investigated the anti-inflammatory effects of pseudocoptisine, a quaternary alkaloid with a benzylisoquinoline skeleton, which was isolated from the tubers of Corydalis turtschaninovii by examining its inhibitory effects on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Pseudocoptisine caused dose-dependent reductions in the levels of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2) at both protein and mRNA levels and concomitant decreases in PGE(2) and NO production. In addition, it was found that pseudocoptisine suppressed the production and mRNA expressions of inflammatory cytokines, such as, TNF-alpha and IL-6. Furthermore, molecular data revealed that pseudocoptisine inhibited the LPS-stimulated DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB). Moreover, this effect was accompanied by decreases in the phosphorylation of inhibitory kappaB (IkappaB)-alpha and in the subsequent blocking of p65 subunit of NF-kappaB translocation to the nucleus. In addition, pseudocoptisine dose-dependently inhibited the phosphorylations of ERK and p38. Taken together, these results suggest that pseudocoptisine reduces levels of the pro-inflammatory mediators, such as, iNOS, COX-2, TNF-alpha, and IL-6 through the inhibition of NF-kappaB activation via the suppression of ERK and p38 phosphorylation in RAW 264.7 cells. These findings reveal in part the molecular basis for the anti-inflammatory properties of pseudocoptisine.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University, Hoegi-Dong, Dongdaemun-ku, Seoul 130-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19666143

Citation

Yun, Kyung-Jin, et al. "Quaternary Alkaloid, Pseudocoptisine Isolated From Tubers of Corydalis Turtschaninovi Inhibits LPS-induced Nitric Oxide, PGE(2), and Pro-inflammatory Cytokines Production Via the Down-regulation of NF-kappaB in RAW 264.7 Murine Macrophage Cells." International Immunopharmacology, vol. 9, no. 11, 2009, pp. 1323-31.
Yun KJ, Shin JS, Choi JH, et al. Quaternary alkaloid, pseudocoptisine isolated from tubers of Corydalis turtschaninovi inhibits LPS-induced nitric oxide, PGE(2), and pro-inflammatory cytokines production via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells. Int Immunopharmacol. 2009;9(11):1323-31.
Yun, K. J., Shin, J. S., Choi, J. H., Back, N. I., Chung, H. G., & Lee, K. T. (2009). Quaternary alkaloid, pseudocoptisine isolated from tubers of Corydalis turtschaninovi inhibits LPS-induced nitric oxide, PGE(2), and pro-inflammatory cytokines production via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells. International Immunopharmacology, 9(11), 1323-31. https://doi.org/10.1016/j.intimp.2009.08.001
Yun KJ, et al. Quaternary Alkaloid, Pseudocoptisine Isolated From Tubers of Corydalis Turtschaninovi Inhibits LPS-induced Nitric Oxide, PGE(2), and Pro-inflammatory Cytokines Production Via the Down-regulation of NF-kappaB in RAW 264.7 Murine Macrophage Cells. Int Immunopharmacol. 2009;9(11):1323-31. PubMed PMID: 19666143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quaternary alkaloid, pseudocoptisine isolated from tubers of Corydalis turtschaninovi inhibits LPS-induced nitric oxide, PGE(2), and pro-inflammatory cytokines production via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells. AU - Yun,Kyung-Jin, AU - Shin,Ji-Sun, AU - Choi,Jung-Hye, AU - Back,Nam-In, AU - Chung,Hae-Gon, AU - Lee,Kyung-Tae, Y1 - 2009/08/07/ PY - 2009/05/11/received PY - 2009/07/22/revised PY - 2009/08/03/accepted PY - 2009/8/12/entrez PY - 2009/8/12/pubmed PY - 2010/1/6/medline SP - 1323 EP - 31 JF - International immunopharmacology JO - Int Immunopharmacol VL - 9 IS - 11 N2 - It is well known that pro-inflammatory mediators like nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6) contribute to the courses of many inflammatory diseases. In the present study, the authors investigated the anti-inflammatory effects of pseudocoptisine, a quaternary alkaloid with a benzylisoquinoline skeleton, which was isolated from the tubers of Corydalis turtschaninovii by examining its inhibitory effects on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Pseudocoptisine caused dose-dependent reductions in the levels of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2) at both protein and mRNA levels and concomitant decreases in PGE(2) and NO production. In addition, it was found that pseudocoptisine suppressed the production and mRNA expressions of inflammatory cytokines, such as, TNF-alpha and IL-6. Furthermore, molecular data revealed that pseudocoptisine inhibited the LPS-stimulated DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB). Moreover, this effect was accompanied by decreases in the phosphorylation of inhibitory kappaB (IkappaB)-alpha and in the subsequent blocking of p65 subunit of NF-kappaB translocation to the nucleus. In addition, pseudocoptisine dose-dependently inhibited the phosphorylations of ERK and p38. Taken together, these results suggest that pseudocoptisine reduces levels of the pro-inflammatory mediators, such as, iNOS, COX-2, TNF-alpha, and IL-6 through the inhibition of NF-kappaB activation via the suppression of ERK and p38 phosphorylation in RAW 264.7 cells. These findings reveal in part the molecular basis for the anti-inflammatory properties of pseudocoptisine. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/19666143/Quaternary_alkaloid_pseudocoptisine_isolated_from_tubers_of_Corydalis_turtschaninovi_inhibits_LPS_induced_nitric_oxide_PGE_2__and_pro_inflammatory_cytokines_production_via_the_down_regulation_of_NF_kappaB_in_RAW_264_7_murine_macrophage_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(09)00231-8 DB - PRIME DP - Unbound Medicine ER -