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Dietary lipid composition affects the gene expression of gill Na(+)/K (+)-ATPase alpha1b but not the alpha1a isoform in juvenile fall chinook salmon (Oncorhynchus tshawytscha).
J Comp Physiol B. 2010 Jan; 180(1):141-9.JC

Abstract

We assessed the effects of dietary fatty acid composition on sodium-potassium ATPase (Na(+)/K(+)-ATPase) activity and isoform expression in the gills of juvenile fall chinook salmon, Oncorhynchus tshawytscha by supplementing diets with either anchovy oil (AO) or AO blended with canola oil (CO) so that CO comprised 0% (0CO), 11% (11CO), 22% (22CO), 33% (33CO), 43% (43CO), or 54% (54CO) of the measured dietary lipid content. The effects of diet were assessed in freshwater (FW) following 104 days of diet manipulation, in response to 24-h seawater (SW) transfer at this time, and following an additional 35 days of SW acclimation. Gill Na(+)/K(+)-ATPase activity was not significantly affected by diet at any sampling time, and there were no consistent effects of diet on the expression of the Na(+)/K(+)-ATPase alpha1a isoform. As dietary CO increased, Na(+)/K(+)-ATPase alpha1b mRNA decreased in fish held in FW, with the 43CO and 54CO diet groups having significantly lower levels than fish fed the 0CO and 11CO diets. Twenty-four-hour SW challenge did not affect the expression of the Na(+)/K(+)-ATPase alpha1a isoform in any diet group, but this isoform was down-regulated in all diet groups following 35 days of SW acclimation. Na(+)/K(+)-ATPase alpha1b expression levels increased in response to 24-h SW transfer and SW acclimation only in fish fed the 54CO diet. The effects of the two extreme diets (0CO and 54CO) were also assessed at various time points during 104 days of rearing in FW. Na(+)/K(+)-ATPase alpha1b mRNA levels were greater in fish fed diet 0CO versus those fed diet 54CO at all times during the FW culture period. These data demonstrate that dietary fatty acid composition can influence the gill Na(+)/K(+)-ATPase isoform physiology of juvenile fall-run chinook salmon prior to SW transfer.

Authors+Show Affiliations

Department of Zoology, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada. amelia@zoology.ubc.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19669152

Citation

Grant, Amelia A M., et al. "Dietary Lipid Composition Affects the Gene Expression of Gill Na(+)/K (+)-ATPase Alpha1b but Not the Alpha1a Isoform in Juvenile Fall Chinook Salmon (Oncorhynchus Tshawytscha)." Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, vol. 180, no. 1, 2010, pp. 141-9.
Grant AA, Higgs DA, Brauner CJ, et al. Dietary lipid composition affects the gene expression of gill Na(+)/K (+)-ATPase alpha1b but not the alpha1a isoform in juvenile fall chinook salmon (Oncorhynchus tshawytscha). J Comp Physiol B. 2010;180(1):141-9.
Grant, A. A., Higgs, D. A., Brauner, C. J., & Schulte, P. M. (2010). Dietary lipid composition affects the gene expression of gill Na(+)/K (+)-ATPase alpha1b but not the alpha1a isoform in juvenile fall chinook salmon (Oncorhynchus tshawytscha). Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, 180(1), 141-9. https://doi.org/10.1007/s00360-009-0394-9
Grant AA, et al. Dietary Lipid Composition Affects the Gene Expression of Gill Na(+)/K (+)-ATPase Alpha1b but Not the Alpha1a Isoform in Juvenile Fall Chinook Salmon (Oncorhynchus Tshawytscha). J Comp Physiol B. 2010;180(1):141-9. PubMed PMID: 19669152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary lipid composition affects the gene expression of gill Na(+)/K (+)-ATPase alpha1b but not the alpha1a isoform in juvenile fall chinook salmon (Oncorhynchus tshawytscha). AU - Grant,Amelia A M, AU - Higgs,Dave A, AU - Brauner,Colin J, AU - Schulte,Patricia M, Y1 - 2009/08/08/ PY - 2009/04/08/received PY - 2009/07/20/accepted PY - 2009/07/17/revised PY - 2009/8/12/entrez PY - 2009/8/12/pubmed PY - 2010/3/17/medline SP - 141 EP - 9 JF - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology JO - J Comp Physiol B VL - 180 IS - 1 N2 - We assessed the effects of dietary fatty acid composition on sodium-potassium ATPase (Na(+)/K(+)-ATPase) activity and isoform expression in the gills of juvenile fall chinook salmon, Oncorhynchus tshawytscha by supplementing diets with either anchovy oil (AO) or AO blended with canola oil (CO) so that CO comprised 0% (0CO), 11% (11CO), 22% (22CO), 33% (33CO), 43% (43CO), or 54% (54CO) of the measured dietary lipid content. The effects of diet were assessed in freshwater (FW) following 104 days of diet manipulation, in response to 24-h seawater (SW) transfer at this time, and following an additional 35 days of SW acclimation. Gill Na(+)/K(+)-ATPase activity was not significantly affected by diet at any sampling time, and there were no consistent effects of diet on the expression of the Na(+)/K(+)-ATPase alpha1a isoform. As dietary CO increased, Na(+)/K(+)-ATPase alpha1b mRNA decreased in fish held in FW, with the 43CO and 54CO diet groups having significantly lower levels than fish fed the 0CO and 11CO diets. Twenty-four-hour SW challenge did not affect the expression of the Na(+)/K(+)-ATPase alpha1a isoform in any diet group, but this isoform was down-regulated in all diet groups following 35 days of SW acclimation. Na(+)/K(+)-ATPase alpha1b expression levels increased in response to 24-h SW transfer and SW acclimation only in fish fed the 54CO diet. The effects of the two extreme diets (0CO and 54CO) were also assessed at various time points during 104 days of rearing in FW. Na(+)/K(+)-ATPase alpha1b mRNA levels were greater in fish fed diet 0CO versus those fed diet 54CO at all times during the FW culture period. These data demonstrate that dietary fatty acid composition can influence the gill Na(+)/K(+)-ATPase isoform physiology of juvenile fall-run chinook salmon prior to SW transfer. SN - 1432-136X UR - https://www.unboundmedicine.com/medline/citation/19669152/Dietary_lipid_composition_affects_the_gene_expression_of_gill_Na_+_/K__+__ATPase_alpha1b_but_not_the_alpha1a_isoform_in_juvenile_fall_chinook_salmon__Oncorhynchus_tshawytscha__ L2 - http://dx.doi.org/10.1007/s00360-009-0394-9 DB - PRIME DP - Unbound Medicine ER -