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TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP.
Pflugers Arch. 2009 Oct; 458(6):1093-102.PA

Abstract

Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) neurons. Here we show that adenosine triphosphate (ATP) is such a candidate molecule released from keratinocytes upon heating in the co-culture systems. Using TRPV1-deficient DRG neurons, we found that increase in cytosolic Ca(2+)-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X(2) cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin.

Authors+Show Affiliations

Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institute for Physiological Sciences, Okazaki, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19669158

Citation

Mandadi, Sravan, et al. "TRPV3 in Keratinocytes Transmits Temperature Information to Sensory Neurons Via ATP." Pflugers Archiv : European Journal of Physiology, vol. 458, no. 6, 2009, pp. 1093-102.
Mandadi S, Sokabe T, Shibasaki K, et al. TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP. Pflugers Arch. 2009;458(6):1093-102.
Mandadi, S., Sokabe, T., Shibasaki, K., Katanosaka, K., Mizuno, A., Moqrich, A., Patapoutian, A., Fukumi-Tominaga, T., Mizumura, K., & Tominaga, M. (2009). TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP. Pflugers Archiv : European Journal of Physiology, 458(6), 1093-102. https://doi.org/10.1007/s00424-009-0703-x
Mandadi S, et al. TRPV3 in Keratinocytes Transmits Temperature Information to Sensory Neurons Via ATP. Pflugers Arch. 2009;458(6):1093-102. PubMed PMID: 19669158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPV3 in keratinocytes transmits temperature information to sensory neurons via ATP. AU - Mandadi,Sravan, AU - Sokabe,Takaaki, AU - Shibasaki,Koji, AU - Katanosaka,Kimiaki, AU - Mizuno,Atsuko, AU - Moqrich,Aziz, AU - Patapoutian,Ardem, AU - Fukumi-Tominaga,Tomoko, AU - Mizumura,Kazue, AU - Tominaga,Makoto, Y1 - 2009/08/08/ PY - 2009/07/13/received PY - 2009/07/15/accepted PY - 2009/8/12/entrez PY - 2009/8/12/pubmed PY - 2010/1/1/medline SP - 1093 EP - 102 JF - Pflugers Archiv : European journal of physiology JO - Pflugers Arch VL - 458 IS - 6 N2 - Transient receptor potential V3 (TRPV3) and TRPV4 are heat-activated cation channels expressed in keratinocytes. It has been proposed that heat-activation of TRPV3 and/or TRPV4 in the skin may release diffusible molecules which would then activate termini of neighboring dorsal root ganglion (DRG) neurons. Here we show that adenosine triphosphate (ATP) is such a candidate molecule released from keratinocytes upon heating in the co-culture systems. Using TRPV1-deficient DRG neurons, we found that increase in cytosolic Ca(2+)-concentration in DRG neurons upon heating was observed only when neurons were co-cultured with keratinocytes, and this increase was blocked by P2 purinoreceptor antagonists, PPADS and suramin. In a co-culture of keratinocytes with HEK293 cells (transfected with P2X(2) cDNA to serve as a bio-sensor), we observed that heat-activated keratinocytes secretes ATP, and that ATP release is compromised in keratinocytes from TRPV3-deficient mice. This study provides evidence that ATP is a messenger molecule for mainly TRPV3-mediated thermotransduction in skin. SN - 1432-2013 UR - https://www.unboundmedicine.com/medline/citation/19669158/TRPV3_in_keratinocytes_transmits_temperature_information_to_sensory_neurons_via_ATP_ L2 - https://dx.doi.org/10.1007/s00424-009-0703-x DB - PRIME DP - Unbound Medicine ER -