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Fast analysis of isobaric grape anthocyanins by Chip-liquid chromatography/mass spectrometry.
Rapid Commun Mass Spectrom. 2009 Sep; 23(18):2891-6.RC

Abstract

In a previous work, direct-infusion electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was applied to the study of anthocyanins in extracts from the skins of Clinton grapes, a non-Vitis vinifera red grape variety qualitatively and quantitatively rich in anthocyanins. A good characterization of anthocyaninins was obtained, but it was impossible to differentiate some compounds with the same nominal mass but with different elemental composition. In this work, the capabilities of quadrupole time-of-flight mass spectrometry (QTOF-MS) coupled with Chip-liquid chromatography (LC-Chip) were applied to the study of Clinton anthocyanins and this method provided the complete sample anthocyanin fingerprint in less than 5 min. Multi-stage mass spectrometry (MS(n); n >2) was not necessary to identify isobaric compounds, nor were deuterium-exchange experiments necessary to distinguish between compounds containing the same aglycone. The fast separation bypasses the problem of petunidin-3-O-(6-O-acetyl)monoglucoside and delphinidin-3,5-O-diglucoside quantification, present in the direct-infusion ESI-ITMS approach, due to overlapping with matrix interferences.

Authors+Show Affiliations

CRA-VIT Laboratorio Chimico, Viale XXVIII aprile 26, 31015 Conegliano (TV), Italy. riccardo.flamini@entecra.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19670341

Citation

Flamini, Riccardo, et al. "Fast Analysis of Isobaric Grape Anthocyanins By Chip-liquid Chromatography/mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 18, 2009, pp. 2891-6.
Flamini R, De Rosso M, Smaniotto A, et al. Fast analysis of isobaric grape anthocyanins by Chip-liquid chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2009;23(18):2891-6.
Flamini, R., De Rosso, M., Smaniotto, A., Panighel, A., Vedova, A. D., Seraglia, R., & Traldi, P. (2009). Fast analysis of isobaric grape anthocyanins by Chip-liquid chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 23(18), 2891-6. https://doi.org/10.1002/rcm.4199
Flamini R, et al. Fast Analysis of Isobaric Grape Anthocyanins By Chip-liquid Chromatography/mass Spectrometry. Rapid Commun Mass Spectrom. 2009;23(18):2891-6. PubMed PMID: 19670341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast analysis of isobaric grape anthocyanins by Chip-liquid chromatography/mass spectrometry. AU - Flamini,Riccardo, AU - De Rosso,Mirko, AU - Smaniotto,Anna, AU - Panighel,Annarita, AU - Vedova,Antonio Dalla, AU - Seraglia,Roberta, AU - Traldi,Pietro, PY - 2009/8/12/entrez PY - 2009/8/12/pubmed PY - 2009/10/21/medline SP - 2891 EP - 6 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 18 N2 - In a previous work, direct-infusion electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was applied to the study of anthocyanins in extracts from the skins of Clinton grapes, a non-Vitis vinifera red grape variety qualitatively and quantitatively rich in anthocyanins. A good characterization of anthocyaninins was obtained, but it was impossible to differentiate some compounds with the same nominal mass but with different elemental composition. In this work, the capabilities of quadrupole time-of-flight mass spectrometry (QTOF-MS) coupled with Chip-liquid chromatography (LC-Chip) were applied to the study of Clinton anthocyanins and this method provided the complete sample anthocyanin fingerprint in less than 5 min. Multi-stage mass spectrometry (MS(n); n >2) was not necessary to identify isobaric compounds, nor were deuterium-exchange experiments necessary to distinguish between compounds containing the same aglycone. The fast separation bypasses the problem of petunidin-3-O-(6-O-acetyl)monoglucoside and delphinidin-3,5-O-diglucoside quantification, present in the direct-infusion ESI-ITMS approach, due to overlapping with matrix interferences. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/19670341/Fast_analysis_of_isobaric_grape_anthocyanins_by_Chip_liquid_chromatography/mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.4199 DB - PRIME DP - Unbound Medicine ER -