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Aptamer-linked assay for thrombin using gold nanoparticle amplification and inductively coupled plasma-mass spectrometry detection.
Anal Chem. 2009 Sep 01; 81(17):7484-9.AC

Abstract

We describe a sensitive and specific sandwich assay for human alpha-thrombin. The assay takes advantage of sandwich binding of two affinity aptamers for increased specificity, gold nanoparticles for signal amplification, magnetic beads for fast magnetic separation, and inductively coupled plasma mass spectrometry for ultrasensitive detection. Other proteins, such as immunoglobulin G, serum albumin, transferrin, fibrinogen, and lysozyme did not show interference with the assay for human alpha-thrombin. The detection limit of human alpha-thrombin was as low as 0.5 fmol, corresponding to 10 pM thrombin in 50 microL, and the dynamic range covered approximately 3 orders of magnitude.

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Authors+Show Affiliations

Zhao Q
Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada T6G 2G3.
Lu X
No affiliation info available
Yuan CG
No affiliation info available
Li XF
No affiliation info available
Le XC
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesGoldHumansMagneticsMass SpectrometryMetal NanoparticlesNanoparticlesSensitivity and SpecificityThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19670869

Citation

Zhao, Qiang, et al. "Aptamer-linked Assay for Thrombin Using Gold Nanoparticle Amplification and Inductively Coupled Plasma-mass Spectrometry Detection." Analytical Chemistry, vol. 81, no. 17, 2009, pp. 7484-9.
Zhao Q, Lu X, Yuan CG, et al. Aptamer-linked assay for thrombin using gold nanoparticle amplification and inductively coupled plasma-mass spectrometry detection. Anal Chem. 2009;81(17):7484-9.
Zhao, Q., Lu, X., Yuan, C. G., Li, X. F., & Le, X. C. (2009). Aptamer-linked assay for thrombin using gold nanoparticle amplification and inductively coupled plasma-mass spectrometry detection. Analytical Chemistry, 81(17), 7484-9. https://doi.org/10.1021/ac900961y
Zhao Q, et al. Aptamer-linked Assay for Thrombin Using Gold Nanoparticle Amplification and Inductively Coupled Plasma-mass Spectrometry Detection. Anal Chem. 2009 Sep 1;81(17):7484-9. PubMed PMID: 19670869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer-linked assay for thrombin using gold nanoparticle amplification and inductively coupled plasma-mass spectrometry detection. AU - Zhao,Qiang, AU - Lu,Xiufen, AU - Yuan,Chun-Gang, AU - Li,Xing-Fang, AU - Le,X Chris, PY - 2009/8/13/entrez PY - 2009/8/13/pubmed PY - 2009/11/17/medline SP - 7484 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 17 N2 - We describe a sensitive and specific sandwich assay for human alpha-thrombin. The assay takes advantage of sandwich binding of two affinity aptamers for increased specificity, gold nanoparticles for signal amplification, magnetic beads for fast magnetic separation, and inductively coupled plasma mass spectrometry for ultrasensitive detection. Other proteins, such as immunoglobulin G, serum albumin, transferrin, fibrinogen, and lysozyme did not show interference with the assay for human alpha-thrombin. The detection limit of human alpha-thrombin was as low as 0.5 fmol, corresponding to 10 pM thrombin in 50 microL, and the dynamic range covered approximately 3 orders of magnitude. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19670869/Aptamer_linked_assay_for_thrombin_using_gold_nanoparticle_amplification_and_inductively_coupled_plasma_mass_spectrometry_detection_ L2 - https://doi.org/10.1021/ac900961y DB - PRIME DP - Unbound Medicine ER -