Tags

Type your tag names separated by a space and hit enter

Estrogen receptor 1 mRNA is a prognostic factor in ovarian carcinoma: determination by kinetic PCR in formalin-fixed paraffin-embedded tissue.
Endocr Relat Cancer. 2009 Dec; 16(4):1229-39.ER

Abstract

Epidemiological and cell culture studies indicate that ovarian carcinoma growth is dependent on estrogen stimulation. However, possibly due to the lack of a reliable biomarker that helps to select patients according to prognostically relevant estrogen receptor (ER) levels, clinical trials using anti-estrogenic therapeutics in ovarian carcinoma have had inconsistent results. Therefore, we tested if ER expression analysis by a quantitative method might be useful in this regard in formalin-fixed paraffin-embedded (FFPE) tissue. In a study group of 114 primary ovarian carcinomas expression of estrogen receptor 1 (ESR1) mRNA was analyzed using a new method for RNA extraction from FFPE tissue that is based on magnetic beads, followed by kinetic PCR. The prognostic impact of ESR1 mRNA expression was investigated and compared to ERalpha protein expression as determined by immunohistochemistry. In univariate survival analysis the expression level of ESR1 mRNA was a significant positive prognostic factor for patient survival (hazard ratio (HR) 0.230 (confidence interval (CI) 0.102-0.516), P=0.002). ERalpha protein expression was correlated to ESR1 mRNA expression (P=0.0001); however, ERalpha protein expression did not provide statistically significant prognostic information. In multivariate analysis, ESR1 mRNA expression emerged as a prognostic factor, independent of stage, grade, residual tumor mass, age, and ERalpha protein expression (HR 0.227 (CI 0.078-0.656), P=0.006). Our results indicate that the determination of ESR1 levels by kinetic PCR may be superior to immunohistochemical methods in assessment of biologically relevant levels of ER expression in ovarian carcinoma, and is feasible in routinely used FFPE tissue.

Authors+Show Affiliations

Institute of Pathology, Charité Universitätsmedizin Berlin, Berlin, Germany. silvia.darb-esfahani@charite.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19675076

Citation

Darb-Esfahani, Silvia, et al. "Estrogen Receptor 1 mRNA Is a Prognostic Factor in Ovarian Carcinoma: Determination By Kinetic PCR in Formalin-fixed Paraffin-embedded Tissue." Endocrine-related Cancer, vol. 16, no. 4, 2009, pp. 1229-39.
Darb-Esfahani S, Wirtz RM, Sinn BV, et al. Estrogen receptor 1 mRNA is a prognostic factor in ovarian carcinoma: determination by kinetic PCR in formalin-fixed paraffin-embedded tissue. Endocr Relat Cancer. 2009;16(4):1229-39.
Darb-Esfahani, S., Wirtz, R. M., Sinn, B. V., Budczies, J., Noske, A., Weichert, W., Faggad, A., Scharff, S., Sehouli, J., Oskay-Ozcelik, G., Zamagni, C., De Iaco, P., Martoni, A., Dietel, M., & Denkert, C. (2009). Estrogen receptor 1 mRNA is a prognostic factor in ovarian carcinoma: determination by kinetic PCR in formalin-fixed paraffin-embedded tissue. Endocrine-related Cancer, 16(4), 1229-39. https://doi.org/10.1677/ERC-08-0338
Darb-Esfahani S, et al. Estrogen Receptor 1 mRNA Is a Prognostic Factor in Ovarian Carcinoma: Determination By Kinetic PCR in Formalin-fixed Paraffin-embedded Tissue. Endocr Relat Cancer. 2009;16(4):1229-39. PubMed PMID: 19675076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen receptor 1 mRNA is a prognostic factor in ovarian carcinoma: determination by kinetic PCR in formalin-fixed paraffin-embedded tissue. AU - Darb-Esfahani,Silvia, AU - Wirtz,Ralph M, AU - Sinn,Bruno V, AU - Budczies,Jan, AU - Noske,Aurelia, AU - Weichert,Wilko, AU - Faggad,Areeg, AU - Scharff,Susanne, AU - Sehouli,Jalid, AU - Oskay-Ozcelik,Guelten, AU - Zamagni,Claudio, AU - De Iaco,Pierandrea, AU - Martoni,Andrea, AU - Dietel,Manfred, AU - Denkert,Carsten, Y1 - 2009/08/12/ PY - 2009/8/14/entrez PY - 2009/8/14/pubmed PY - 2010/2/17/medline SP - 1229 EP - 39 JF - Endocrine-related cancer JO - Endocr Relat Cancer VL - 16 IS - 4 N2 - Epidemiological and cell culture studies indicate that ovarian carcinoma growth is dependent on estrogen stimulation. However, possibly due to the lack of a reliable biomarker that helps to select patients according to prognostically relevant estrogen receptor (ER) levels, clinical trials using anti-estrogenic therapeutics in ovarian carcinoma have had inconsistent results. Therefore, we tested if ER expression analysis by a quantitative method might be useful in this regard in formalin-fixed paraffin-embedded (FFPE) tissue. In a study group of 114 primary ovarian carcinomas expression of estrogen receptor 1 (ESR1) mRNA was analyzed using a new method for RNA extraction from FFPE tissue that is based on magnetic beads, followed by kinetic PCR. The prognostic impact of ESR1 mRNA expression was investigated and compared to ERalpha protein expression as determined by immunohistochemistry. In univariate survival analysis the expression level of ESR1 mRNA was a significant positive prognostic factor for patient survival (hazard ratio (HR) 0.230 (confidence interval (CI) 0.102-0.516), P=0.002). ERalpha protein expression was correlated to ESR1 mRNA expression (P=0.0001); however, ERalpha protein expression did not provide statistically significant prognostic information. In multivariate analysis, ESR1 mRNA expression emerged as a prognostic factor, independent of stage, grade, residual tumor mass, age, and ERalpha protein expression (HR 0.227 (CI 0.078-0.656), P=0.006). Our results indicate that the determination of ESR1 levels by kinetic PCR may be superior to immunohistochemical methods in assessment of biologically relevant levels of ER expression in ovarian carcinoma, and is feasible in routinely used FFPE tissue. SN - 1479-6821 UR - https://www.unboundmedicine.com/medline/citation/19675076/Estrogen_receptor_1_mRNA_is_a_prognostic_factor_in_ovarian_carcinoma:_determination_by_kinetic_PCR_in_formalin_fixed_paraffin_embedded_tissue_ L2 - https://erc.bioscientifica.com/doi/10.1677/ERC-08-0338 DB - PRIME DP - Unbound Medicine ER -