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Detection of Coccidioides antigenemia following dissociation of immune complexes.
Clin Vaccine Immunol. 2009 Oct; 16(10):1453-6.CV

Abstract

Having reported that pretreatment of serum samples with EDTA at 100 degrees C improved the sensitivity for the detection of Histoplasma antigenemia, we have evaluated this method for the detection of Coccidioides antigenemia. Urine and serum samples from patients with coccidioidomycosis were tested using the MVista Coccidioides enzyme immunoassay, and serum samples with and without EDTA-heat treatment were tested. Antigenemia was detected in 28.6% of patients whose samples were not EDTA-heat treated and in 73.1% of those whose samples were treated. Antigenuria was detected in 50% of patients. Specificity of 100% was obtained in healthy subjects, but cross-reactions were seen in 22.2% of patients with histoplasmosis or blastomycosis. EDTA-heat treatment improves the sensitivity for the detection of Coccidioides antigenemia.

Authors+Show Affiliations

MiraVista Diagnostics, Indianapolis, Indiana1; MiraBella Technologies, Indianapolis, Indiana 46241, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19675225

Citation

Durkin, Michelle, et al. "Detection of Coccidioides Antigenemia Following Dissociation of Immune Complexes." Clinical and Vaccine Immunology : CVI, vol. 16, no. 10, 2009, pp. 1453-6.
Durkin M, Estok L, Hospenthal D, et al. Detection of Coccidioides antigenemia following dissociation of immune complexes. Clin Vaccine Immunol. 2009;16(10):1453-6.
Durkin, M., Estok, L., Hospenthal, D., Crum-Cianflone, N., Swartzentruber, S., Hackett, E., & Wheat, L. J. (2009). Detection of Coccidioides antigenemia following dissociation of immune complexes. Clinical and Vaccine Immunology : CVI, 16(10), 1453-6. https://doi.org/10.1128/CVI.00227-09
Durkin M, et al. Detection of Coccidioides Antigenemia Following Dissociation of Immune Complexes. Clin Vaccine Immunol. 2009;16(10):1453-6. PubMed PMID: 19675225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Coccidioides antigenemia following dissociation of immune complexes. AU - Durkin,Michelle, AU - Estok,Lois, AU - Hospenthal,Duane, AU - Crum-Cianflone,Nancy, AU - Swartzentruber,Samantha, AU - Hackett,Emily, AU - Wheat,L Joseph, Y1 - 2009/08/12/ PY - 2009/8/14/entrez PY - 2009/8/14/pubmed PY - 2009/12/30/medline SP - 1453 EP - 6 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 16 IS - 10 N2 - Having reported that pretreatment of serum samples with EDTA at 100 degrees C improved the sensitivity for the detection of Histoplasma antigenemia, we have evaluated this method for the detection of Coccidioides antigenemia. Urine and serum samples from patients with coccidioidomycosis were tested using the MVista Coccidioides enzyme immunoassay, and serum samples with and without EDTA-heat treatment were tested. Antigenemia was detected in 28.6% of patients whose samples were not EDTA-heat treated and in 73.1% of those whose samples were treated. Antigenuria was detected in 50% of patients. Specificity of 100% was obtained in healthy subjects, but cross-reactions were seen in 22.2% of patients with histoplasmosis or blastomycosis. EDTA-heat treatment improves the sensitivity for the detection of Coccidioides antigenemia. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/19675225/Detection_of_Coccidioides_antigenemia_following_dissociation_of_immune_complexes_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=19675225 DB - PRIME DP - Unbound Medicine ER -