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A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.
Diagn Microbiol Infect Dis. 2009 Sep; 65(1):73-5.DM

Abstract

Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.

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Authors+Show Affiliations

Couto MS
Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará CEP: 60.425-540, Brazil.
Cordeiro Rde A
No affiliation info available
Rocha MF
No affiliation info available
Grangeiro TB
No affiliation info available
Leitão Junior NP
No affiliation info available
Bandeira Tde J
No affiliation info available
Sidrim JJ
No affiliation info available
Brilhante RS
No affiliation info available

MeSH

Bronchoalveolar Lavage FluidBurkholderia pseudomalleiDNA PrimersDNA, BacterialDNA, Ribosomal SpacerHumansMelioidosisPolymerase Chain ReactionSensitivity and SpecificityUnited States

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19679240

Citation

Couto, Manuela Soares, et al. "A Diagnosis of Burkholderia Pseudomallei Directly in a Bronchoalveolar Lavage By Polymerase Chain Reaction." Diagnostic Microbiology and Infectious Disease, vol. 65, no. 1, 2009, pp. 73-5.
Couto MS, Cordeiro Rde A, Rocha MF, et al. A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. Diagn Microbiol Infect Dis. 2009;65(1):73-5.
Couto, M. S., Cordeiro, R. d. e. . A., Rocha, M. F., Grangeiro, T. B., Leitão Junior, N. P., Bandeira, T. d. e. . J., Sidrim, J. J., & Brilhante, R. S. (2009). A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. Diagnostic Microbiology and Infectious Disease, 65(1), 73-5. https://doi.org/10.1016/j.diagmicrobio.2009.05.010
Couto MS, et al. A Diagnosis of Burkholderia Pseudomallei Directly in a Bronchoalveolar Lavage By Polymerase Chain Reaction. Diagn Microbiol Infect Dis. 2009;65(1):73-5. PubMed PMID: 19679240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. AU - Couto,Manuela Soares, AU - Cordeiro,Rossana de Aguiar, AU - Rocha,Marcos Fábio Gadelha, AU - Grangeiro,Thalles Barbosa, AU - Leitão Junior,Natanael Pinheiro, AU - Bandeira,Tereza de Jesus Pinheiro Gomes, AU - Sidrim,José Júlio Costa, AU - Brilhante,Raimunda Sâmia Nogueira, PY - 2009/02/05/received PY - 2009/05/04/revised PY - 2009/05/11/accepted PY - 2009/8/15/entrez PY - 2009/8/15/pubmed PY - 2009/10/10/medline SP - 73 EP - 5 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 65 IS - 1 N2 - Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/19679240/A_diagnosis_of_Burkholderia_pseudomallei_directly_in_a_bronchoalveolar_lavage_by_polymerase_chain_reaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(09)00208-9 DB - PRIME DP - Unbound Medicine ER -