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A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.
Diagn Microbiol Infect Dis. 2009 Sep; 65(1):73-5.DM

Abstract

Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.

Authors+Show Affiliations

Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza, Ceará CEP: 60.425-540, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19679240

Citation

Couto, Manuela Soares, et al. "A Diagnosis of Burkholderia Pseudomallei Directly in a Bronchoalveolar Lavage By Polymerase Chain Reaction." Diagnostic Microbiology and Infectious Disease, vol. 65, no. 1, 2009, pp. 73-5.
Couto MS, Cordeiro Rde A, Rocha MF, et al. A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. Diagn Microbiol Infect Dis. 2009;65(1):73-5.
Couto, M. S., Cordeiro, R. d. e. . A., Rocha, M. F., Grangeiro, T. B., Leitão Junior, N. P., Bandeira, T. d. e. . J., Sidrim, J. J., & Brilhante, R. S. (2009). A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. Diagnostic Microbiology and Infectious Disease, 65(1), 73-5. https://doi.org/10.1016/j.diagmicrobio.2009.05.010
Couto MS, et al. A Diagnosis of Burkholderia Pseudomallei Directly in a Bronchoalveolar Lavage By Polymerase Chain Reaction. Diagn Microbiol Infect Dis. 2009;65(1):73-5. PubMed PMID: 19679240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction. AU - Couto,Manuela Soares, AU - Cordeiro,Rossana de Aguiar, AU - Rocha,Marcos Fábio Gadelha, AU - Grangeiro,Thalles Barbosa, AU - Leitão Junior,Natanael Pinheiro, AU - Bandeira,Tereza de Jesus Pinheiro Gomes, AU - Sidrim,José Júlio Costa, AU - Brilhante,Raimunda Sâmia Nogueira, PY - 2009/02/05/received PY - 2009/05/04/revised PY - 2009/05/11/accepted PY - 2009/8/15/entrez PY - 2009/8/15/pubmed PY - 2009/10/10/medline SP - 73 EP - 5 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 65 IS - 1 N2 - Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/19679240/A_diagnosis_of_Burkholderia_pseudomallei_directly_in_a_bronchoalveolar_lavage_by_polymerase_chain_reaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(09)00208-9 DB - PRIME DP - Unbound Medicine ER -