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Solid-phase extraction clean-up of ciguatoxin-contaminated coral fish extracts for use in the mouse bioassay.

Abstract

Florisil solid-phase extraction (SPE) cartridges were used for purifying ciguatoxin (CTX)-contaminated coral fish extracts, with the aim of removing extracted lipid but retaining optimal level of CTXs in the purified fractions. The CTX-containing fraction (target fraction) in fish ether extract was isolated and purified by eluting through a commercially available Florisil cartridge with hexane-acetone-methanol solvent mixtures of increasing polarity (hexane-acetone (4:1, v/v) < acetone-methanol (7:3, v/v) < 100% methanol). Application of Florisil SPE using acetone-methanol (7:3, v/v) condition facilitated the separation of 4.2 +/- 0.4 mg (mean +/- standard error of the mean (SEM)) of purified target fraction from 20 mg ether extract with good retention of CTXs. The mouse bioassay was used to demonstrate that the average CTX recovery of the target fraction from CTX-spiked samples was 75.8% +/- 3.3%, which was significantly increased by 96.7% +/- 15% when compared with CTX recovery from ether extracts (44.8% +/- 5.2%) without performing SPE purification. Over 70% of non-target lipids were removed in which no CTX toxicity was found. Moreover, the target fractions of both CTX-spiked and naturally CTX-contaminated samples gave more prominent toxic responses of hypothermia and/or induced more rapid death of the mice. The use of acetone-methanol (7:3, v/v) condition in the elution could significantly improve overall recovery of CTXs, while minimizing the possible interferences of lipid matrix from co-extractants on mice.

Authors+Show Affiliations

Biotoxin Laboratory, Public Health Laboratory Services Branch, Centre for Health Protection, Kowloon, Hong Kong, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19680895

Citation

Wong, Chun Kwan, et al. "Solid-phase Extraction Clean-up of Ciguatoxin-contaminated Coral Fish Extracts for Use in the Mouse Bioassay." Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, vol. 26, no. 2, 2009, pp. 236-47.
Wong CK, Hung P, Lee KL, et al. Solid-phase extraction clean-up of ciguatoxin-contaminated coral fish extracts for use in the mouse bioassay. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009;26(2):236-47.
Wong, C. K., Hung, P., Lee, K. L., & Kam, K. M. (2009). Solid-phase extraction clean-up of ciguatoxin-contaminated coral fish extracts for use in the mouse bioassay. Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 26(2), 236-47. https://doi.org/10.1080/02652030802342505
Wong CK, et al. Solid-phase Extraction Clean-up of Ciguatoxin-contaminated Coral Fish Extracts for Use in the Mouse Bioassay. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009;26(2):236-47. PubMed PMID: 19680895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-phase extraction clean-up of ciguatoxin-contaminated coral fish extracts for use in the mouse bioassay. AU - Wong,Chun Kwan, AU - Hung,Patricia, AU - Lee,Kellie L H, AU - Kam,Kai Man, PY - 2009/8/15/entrez PY - 2009/8/15/pubmed PY - 2010/5/15/medline SP - 236 EP - 47 JF - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment JO - Food Addit Contam Part A Chem Anal Control Expo Risk Assess VL - 26 IS - 2 N2 - Florisil solid-phase extraction (SPE) cartridges were used for purifying ciguatoxin (CTX)-contaminated coral fish extracts, with the aim of removing extracted lipid but retaining optimal level of CTXs in the purified fractions. The CTX-containing fraction (target fraction) in fish ether extract was isolated and purified by eluting through a commercially available Florisil cartridge with hexane-acetone-methanol solvent mixtures of increasing polarity (hexane-acetone (4:1, v/v) < acetone-methanol (7:3, v/v) < 100% methanol). Application of Florisil SPE using acetone-methanol (7:3, v/v) condition facilitated the separation of 4.2 +/- 0.4 mg (mean +/- standard error of the mean (SEM)) of purified target fraction from 20 mg ether extract with good retention of CTXs. The mouse bioassay was used to demonstrate that the average CTX recovery of the target fraction from CTX-spiked samples was 75.8% +/- 3.3%, which was significantly increased by 96.7% +/- 15% when compared with CTX recovery from ether extracts (44.8% +/- 5.2%) without performing SPE purification. Over 70% of non-target lipids were removed in which no CTX toxicity was found. Moreover, the target fractions of both CTX-spiked and naturally CTX-contaminated samples gave more prominent toxic responses of hypothermia and/or induced more rapid death of the mice. The use of acetone-methanol (7:3, v/v) condition in the elution could significantly improve overall recovery of CTXs, while minimizing the possible interferences of lipid matrix from co-extractants on mice. SN - 1944-0057 UR - https://www.unboundmedicine.com/medline/citation/19680895/Solid_phase_extraction_clean_up_of_ciguatoxin_contaminated_coral_fish_extracts_for_use_in_the_mouse_bioassay_ L2 - https://www.tandfonline.com/doi/full/10.1080/02652030802342505 DB - PRIME DP - Unbound Medicine ER -