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A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos.
Plant J. 2009 Oct; 60(2):227-41.PJ

Abstract

Indole-3-acetic acid (IAA) plays a critical role in many aspects of plant growth and development; however, complete pathways of biosynthesis, localization and many aspects of functions of IAA in rice remain unclear. Here, we report the analysis of a rice tryptophan- (Trp-) and IAA-deficient mutant, tryptophan deficient dwarf1 (tdd1), which is embryonic lethal because of a failure to develop most organs during embryogenesis. Regenerated tdd1 plants showed pleiotropic phenotypes: dwarfing, narrow leaves, short roots and abnormal flowers. TDD1 encodes a protein homologous to anthranilate synthase beta-subunit, which catalyses the first step of the Trp biosynthesis pathway and functions upstream of Trp-dependent IAA biosynthesis. TDD1-uidA and DR5-uidA expression overlapped at many sites in WT plants but was lacking in tdd1, indicating that TDD1 is involved in auxin biosynthesis. Both Trp and IAA levels in flowers and embryos were much lower in tdd1 than in wild type (WT). Trp feeding completely rescued the mutant phenotypes and moderate expression of OsYUCCA1, which encodes a key enzyme in Trp-dependent IAA biosynthesis, also rescued plant height and root length, indicating that the abnormal phenotypes of tdd1 are caused predominantly by Trp and IAA deficiency. In tdd1 embryos, the expression patterns of OSH1 and OsSCR, which mark the presumptive apical region and the L2 layer, respectively, are identical to those in WT, suggesting a possibility either that different IAA levels are required for basic pattern formation than for organ formation or that an orthologous gene compensates for TDD1 deficiency during pattern formation.

Authors+Show Affiliations

Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi, Japan. sazuka@agr.nagoya-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19682283

Citation

Sazuka, Takashi, et al. "A Rice Tryptophan Deficient Dwarf Mutant, Tdd1, Contains a Reduced Level of Indole Acetic Acid and Develops Abnormal Flowers and Organless Embryos." The Plant Journal : for Cell and Molecular Biology, vol. 60, no. 2, 2009, pp. 227-41.
Sazuka T, Kamiya N, Nishimura T, et al. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. Plant J. 2009;60(2):227-41.
Sazuka, T., Kamiya, N., Nishimura, T., Ohmae, K., Sato, Y., Imamura, K., Nagato, Y., Koshiba, T., Nagamura, Y., Ashikari, M., Kitano, H., & Matsuoka, M. (2009). A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. The Plant Journal : for Cell and Molecular Biology, 60(2), 227-41. https://doi.org/10.1111/j.1365-313X.2009.03952.x
Sazuka T, et al. A Rice Tryptophan Deficient Dwarf Mutant, Tdd1, Contains a Reduced Level of Indole Acetic Acid and Develops Abnormal Flowers and Organless Embryos. Plant J. 2009;60(2):227-41. PubMed PMID: 19682283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. AU - Sazuka,Takashi, AU - Kamiya,Noriko, AU - Nishimura,Takeshi, AU - Ohmae,Kozue, AU - Sato,Yutaka, AU - Imamura,Kohei, AU - Nagato,Yasuo, AU - Koshiba,Tomokazu, AU - Nagamura,Yoshiaki, AU - Ashikari,Motoyuki, AU - Kitano,Hidemi, AU - Matsuoka,Makoto, Y1 - 2009/06/15/ PY - 2009/8/18/entrez PY - 2009/8/18/pubmed PY - 2009/12/16/medline SP - 227 EP - 41 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 60 IS - 2 N2 - Indole-3-acetic acid (IAA) plays a critical role in many aspects of plant growth and development; however, complete pathways of biosynthesis, localization and many aspects of functions of IAA in rice remain unclear. Here, we report the analysis of a rice tryptophan- (Trp-) and IAA-deficient mutant, tryptophan deficient dwarf1 (tdd1), which is embryonic lethal because of a failure to develop most organs during embryogenesis. Regenerated tdd1 plants showed pleiotropic phenotypes: dwarfing, narrow leaves, short roots and abnormal flowers. TDD1 encodes a protein homologous to anthranilate synthase beta-subunit, which catalyses the first step of the Trp biosynthesis pathway and functions upstream of Trp-dependent IAA biosynthesis. TDD1-uidA and DR5-uidA expression overlapped at many sites in WT plants but was lacking in tdd1, indicating that TDD1 is involved in auxin biosynthesis. Both Trp and IAA levels in flowers and embryos were much lower in tdd1 than in wild type (WT). Trp feeding completely rescued the mutant phenotypes and moderate expression of OsYUCCA1, which encodes a key enzyme in Trp-dependent IAA biosynthesis, also rescued plant height and root length, indicating that the abnormal phenotypes of tdd1 are caused predominantly by Trp and IAA deficiency. In tdd1 embryos, the expression patterns of OSH1 and OsSCR, which mark the presumptive apical region and the L2 layer, respectively, are identical to those in WT, suggesting a possibility either that different IAA levels are required for basic pattern formation than for organ formation or that an orthologous gene compensates for TDD1 deficiency during pattern formation. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/19682283/A_rice_tryptophan_deficient_dwarf_mutant_tdd1_contains_a_reduced_level_of_indole_acetic_acid_and_develops_abnormal_flowers_and_organless_embryos_ L2 - https://doi.org/10.1111/j.1365-313X.2009.03952.x DB - PRIME DP - Unbound Medicine ER -