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Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes.
Virology. 2009 Sep 01; 391(2):203-11.V

Abstract

Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4(+) lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

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Authors+Show Affiliations

Stax MJ
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of the University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
van Montfort T
No affiliation info available
Sprenger RR
No affiliation info available
Melchers M
No affiliation info available
Sanders RW
No affiliation info available
van Leeuwen E
No affiliation info available
Repping S
No affiliation info available
Pollakis G
No affiliation info available
Speijer D
No affiliation info available
Paxton WA
No affiliation info available

MeSH

CD4-Positive T-LymphocytesCell Adhesion MoleculesCell LineCells, CulturedDendritic CellsHIV-1HumansLectins, C-TypeMucin-6Protein BindingReceptors, Cell SurfaceSemen

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19682628

Citation

Stax, Martijn J., et al. "Mucin 6 in Seminal Plasma Binds DC-SIGN and Potently Blocks Dendritic Cell Mediated Transfer of HIV-1 to CD4(+) T-lymphocytes." Virology, vol. 391, no. 2, 2009, pp. 203-11.
Stax MJ, van Montfort T, Sprenger RR, et al. Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes. Virology. 2009;391(2):203-11.
Stax, M. J., van Montfort, T., Sprenger, R. R., Melchers, M., Sanders, R. W., van Leeuwen, E., Repping, S., Pollakis, G., Speijer, D., & Paxton, W. A. (2009). Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes. Virology, 391(2), 203-11. https://doi.org/10.1016/j.virol.2009.06.011
Stax MJ, et al. Mucin 6 in Seminal Plasma Binds DC-SIGN and Potently Blocks Dendritic Cell Mediated Transfer of HIV-1 to CD4(+) T-lymphocytes. Virology. 2009 Sep 1;391(2):203-11. PubMed PMID: 19682628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes. AU - Stax,Martijn J, AU - van Montfort,Thijs, AU - Sprenger,Richard R, AU - Melchers,Mark, AU - Sanders,Rogier W, AU - van Leeuwen,Elisabeth, AU - Repping,Sjoerd, AU - Pollakis,Georgios, AU - Speijer,Dave, AU - Paxton,William A, PY - 2009/03/13/received PY - 2009/04/03/revised PY - 2009/06/06/accepted PY - 2009/8/18/entrez PY - 2009/8/18/pubmed PY - 2009/9/1/medline SP - 203 EP - 11 JF - Virology JO - Virology VL - 391 IS - 2 N2 - Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4(+) lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/19682628/Mucin_6_in_seminal_plasma_binds_DC_SIGN_and_potently_blocks_dendritic_cell_mediated_transfer_of_HIV_1_to_CD4_+__T_lymphocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(09)00355-9 DB - PRIME DP - Unbound Medicine ER -