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Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina.
Clin Microbiol Infect. 2010 Feb; 16(2):152-6.CM

Abstract

Fifty-seven nosocomial Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases (ESBLs) were collected between February 2007 and November 2007 in different wards of the Sarajevo (Bosnia-Herzegovina) reference hospital. These isolates comprise two major epidemic pulsed-field electrophoresis-defined clones plus two minor clones. In addition to the ESBL-mediated resistance, all strains uniformly showed resistance to ciprofloxacin, gentamicin and tobramycin. The beta-lactamases involved in this resistance phenotype were TEM-1, SHV-1, and CTX-M-15, as demonstrated by isoelectric focusing, PCR amplification, and sequencing. TEM-1 and CTX-M-15 beta-lactamases, as well as the aminoglycoside resistance determinants, were encoded in plasmids that could be transferred to Escherichia coli by conjugation. In three of the infected patients with the predominant clone, cefoxitin resistance development (MICs >128 mg/L) was documented. The analysis of the outer membrane proteins of the cefoxitin-susceptible and cefoxitin-resistant isolates revealed that the former expressed only one of the two major porins, OmpK36, whereas in the latter, the expression of Ompk36 was altered or abolished. This is the first report of CTX-M-15-producing K. pneumoniae in Bosnia-Herzegovina. Furthermore, we document and characterize for the first time cefoxitin resistance development in CTX-M-15-producing K. pneumoniae.

Authors+Show Affiliations

Institute of Clinical Microbiology, University of Sarajevo Clinics Centre, Sarajevo, Bosnia and Herzegovina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19686282

Citation

Dedeic-Ljubovic, A, et al. "Emergence of CTX-M-15 Extended-spectrum Beta-lactamase-producing Klebsiella Pneumoniae Isolates in Bosnia and Herzegovina." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 16, no. 2, 2010, pp. 152-6.
Dedeic-Ljubovic A, Hukic M, Pfeifer Y, et al. Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. Clin Microbiol Infect. 2010;16(2):152-6.
Dedeic-Ljubovic, A., Hukic, M., Pfeifer, Y., Witte, W., Padilla, E., López-Ramis, I., & Albertí, S. (2010). Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 16(2), 152-6. https://doi.org/10.1111/j.1469-0691.2009.03018.x
Dedeic-Ljubovic A, et al. Emergence of CTX-M-15 Extended-spectrum Beta-lactamase-producing Klebsiella Pneumoniae Isolates in Bosnia and Herzegovina. Clin Microbiol Infect. 2010;16(2):152-6. PubMed PMID: 19686282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. AU - Dedeic-Ljubovic,A, AU - Hukic,M, AU - Pfeifer,Y, AU - Witte,W, AU - Padilla,E, AU - López-Ramis,I, AU - Albertí,S, Y1 - 2009/08/17/ PY - 2009/8/19/entrez PY - 2009/8/19/pubmed PY - 2010/3/23/medline SP - 152 EP - 6 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin Microbiol Infect VL - 16 IS - 2 N2 - Fifty-seven nosocomial Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases (ESBLs) were collected between February 2007 and November 2007 in different wards of the Sarajevo (Bosnia-Herzegovina) reference hospital. These isolates comprise two major epidemic pulsed-field electrophoresis-defined clones plus two minor clones. In addition to the ESBL-mediated resistance, all strains uniformly showed resistance to ciprofloxacin, gentamicin and tobramycin. The beta-lactamases involved in this resistance phenotype were TEM-1, SHV-1, and CTX-M-15, as demonstrated by isoelectric focusing, PCR amplification, and sequencing. TEM-1 and CTX-M-15 beta-lactamases, as well as the aminoglycoside resistance determinants, were encoded in plasmids that could be transferred to Escherichia coli by conjugation. In three of the infected patients with the predominant clone, cefoxitin resistance development (MICs >128 mg/L) was documented. The analysis of the outer membrane proteins of the cefoxitin-susceptible and cefoxitin-resistant isolates revealed that the former expressed only one of the two major porins, OmpK36, whereas in the latter, the expression of Ompk36 was altered or abolished. This is the first report of CTX-M-15-producing K. pneumoniae in Bosnia-Herzegovina. Furthermore, we document and characterize for the first time cefoxitin resistance development in CTX-M-15-producing K. pneumoniae. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/19686282/Emergence_of_CTX_M_15_extended_spectrum_beta_lactamase_producing_Klebsiella_pneumoniae_isolates_in_Bosnia_and_Herzegovina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)61538-X DB - PRIME DP - Unbound Medicine ER -