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Silver nanoparticles self assembly as SERS substrates with near single molecule detection limit.
Phys Chem Chem Phys. 2009 Sep 14; 11(34):7381-9.PC

Abstract

Highly sensitive SERS substrates with a limit of detection in the zeptomole (for Nile blue A and oxazine 720) range were fabricated through a bottom-up strategy. Ag nanoparticles (Ag NPs) were self-assembled onto glass slides by using 3-mercaptopropyltrimethoxysilane (MPTMS) sol-gel as linker. The substrates were characterized by UV-Vis and AFM after each deposition of Ag NPs. It was found that the glass slide presented just a few Ag NPs aggregates scattered throughout the surface after just one deposition. The glass surface was gradually covered by a homogeneous distribution of Ag NPs aggregates as the deposition number increased. Surface-enhanced Raman scattering (SERS) of the substrates was examined at different numbers of Ag NPs deposition using nile blue A and oxazine 720 as probe molecules and two laser excitations (632.8 nm and 785 nm). Optimum SERS was observed after six depositions of Ag NPs. SERS mapping indicated that at lower deposition numbers (less than 3 Ag NPs depositions) the substrates presented a few SERS "hot-spots" randomly distributed at the surface. After 7 Ag NPs depositions, spatial distribution of the SERS signal followed a Gaussian statistics, with a percent relative standard deviation (RSD%) of approximately 19%. In addition, the sample-to-sample reproducibility of the SERS intensities under both laser excitations was lower than 20%. It was also found that these substrates can provide giant Raman signal enhancement. At optimum conditions and with a 632.8 nm laser, the signal from an estimated of only approximately 44 probe molecules (100x objective) can still be detected.

Authors+Show Affiliations

Department of Chemistry, University of Victoria, Victoria, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19690709

Citation

Fan, Meikun, and Alexandre G. Brolo. "Silver Nanoparticles Self Assembly as SERS Substrates With Near Single Molecule Detection Limit." Physical Chemistry Chemical Physics : PCCP, vol. 11, no. 34, 2009, pp. 7381-9.
Fan M, Brolo AG. Silver nanoparticles self assembly as SERS substrates with near single molecule detection limit. Phys Chem Chem Phys. 2009;11(34):7381-9.
Fan, M., & Brolo, A. G. (2009). Silver nanoparticles self assembly as SERS substrates with near single molecule detection limit. Physical Chemistry Chemical Physics : PCCP, 11(34), 7381-9. https://doi.org/10.1039/b904744a
Fan M, Brolo AG. Silver Nanoparticles Self Assembly as SERS Substrates With Near Single Molecule Detection Limit. Phys Chem Chem Phys. 2009 Sep 14;11(34):7381-9. PubMed PMID: 19690709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles self assembly as SERS substrates with near single molecule detection limit. AU - Fan,Meikun, AU - Brolo,Alexandre G, Y1 - 2009/07/15/ PY - 2009/8/20/entrez PY - 2009/8/20/pubmed PY - 2009/10/20/medline SP - 7381 EP - 9 JF - Physical chemistry chemical physics : PCCP JO - Phys Chem Chem Phys VL - 11 IS - 34 N2 - Highly sensitive SERS substrates with a limit of detection in the zeptomole (for Nile blue A and oxazine 720) range were fabricated through a bottom-up strategy. Ag nanoparticles (Ag NPs) were self-assembled onto glass slides by using 3-mercaptopropyltrimethoxysilane (MPTMS) sol-gel as linker. The substrates were characterized by UV-Vis and AFM after each deposition of Ag NPs. It was found that the glass slide presented just a few Ag NPs aggregates scattered throughout the surface after just one deposition. The glass surface was gradually covered by a homogeneous distribution of Ag NPs aggregates as the deposition number increased. Surface-enhanced Raman scattering (SERS) of the substrates was examined at different numbers of Ag NPs deposition using nile blue A and oxazine 720 as probe molecules and two laser excitations (632.8 nm and 785 nm). Optimum SERS was observed after six depositions of Ag NPs. SERS mapping indicated that at lower deposition numbers (less than 3 Ag NPs depositions) the substrates presented a few SERS "hot-spots" randomly distributed at the surface. After 7 Ag NPs depositions, spatial distribution of the SERS signal followed a Gaussian statistics, with a percent relative standard deviation (RSD%) of approximately 19%. In addition, the sample-to-sample reproducibility of the SERS intensities under both laser excitations was lower than 20%. It was also found that these substrates can provide giant Raman signal enhancement. At optimum conditions and with a 632.8 nm laser, the signal from an estimated of only approximately 44 probe molecules (100x objective) can still be detected. SN - 1463-9076 UR - https://www.unboundmedicine.com/medline/citation/19690709/Silver_nanoparticles_self_assembly_as_SERS_substrates_with_near_single_molecule_detection_limit_ L2 - https://doi.org/10.1039/b904744a DB - PRIME DP - Unbound Medicine ER -