Tags

Type your tag names separated by a space and hit enter

Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis.
J Mol Med (Berl) 2009; 87(11):1111-21JM

Abstract

Inflammatory bowel disease affects millions of individuals; nevertheless, pharmacological treatment is disappointingly unsatisfactory. Cannabidiol, a safe and non-psychotropic ingredient of marijuana, exerts pharmacological effects (e.g., antioxidant) and mechanisms (e.g., inhibition of endocannabinoids enzymatic degradation) potentially beneficial for the inflamed gut. Thus, we investigated the effect of cannabidiol in a murine model of colitis. Colitis was induced in mice by intracolonic administration of dinitrobenzene sulfonic acid. Inflammation was assessed both macroscopically and histologically. In the inflamed colon, cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) were evaluated by Western blot, interleukin-1beta and interleukin-10 by ELISA, and endocannabinoids by isotope dilution liquid chromatography-mass spectrometry. Human colon adenocarcinoma (Caco-2) cells were used to evaluate the effect of cannabidiol on oxidative stress. Cannabidiol reduced colon injury, inducible iNOS (but not cyclooxygenase-2) expression, and interleukin-1beta, interleukin-10, and endocannabinoid changes associated with 2,4,6-dinitrobenzene sulfonic acid administration. In Caco-2 cells, cannabidiol reduced reactive oxygen species production and lipid peroxidation. In conclusion, cannabidiol, a likely safe compound, prevents experimental colitis in mice.

Authors+Show Affiliations

Department of Experimental Pharmacology, University of Naples Federico II, via D Montesano 49, 80131 Naples, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19690824

Citation

Borrelli, Francesca, et al. "Cannabidiol, a Safe and Non-psychotropic Ingredient of the Marijuana Plant Cannabis Sativa, Is Protective in a Murine Model of Colitis." Journal of Molecular Medicine (Berlin, Germany), vol. 87, no. 11, 2009, pp. 1111-21.
Borrelli F, Aviello G, Romano B, et al. Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis. J Mol Med. 2009;87(11):1111-21.
Borrelli, F., Aviello, G., Romano, B., Orlando, P., Capasso, R., Maiello, F., ... Izzo, A. A. (2009). Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis. Journal of Molecular Medicine (Berlin, Germany), 87(11), pp. 1111-21. doi:10.1007/s00109-009-0512-x.
Borrelli F, et al. Cannabidiol, a Safe and Non-psychotropic Ingredient of the Marijuana Plant Cannabis Sativa, Is Protective in a Murine Model of Colitis. J Mol Med. 2009;87(11):1111-21. PubMed PMID: 19690824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis. AU - Borrelli,Francesca, AU - Aviello,Gabriella, AU - Romano,Barbara, AU - Orlando,Pierangelo, AU - Capasso,Raffaele, AU - Maiello,Francesco, AU - Guadagno,Federico, AU - Petrosino,Stefania, AU - Capasso,Francesco, AU - Di Marzo,Vincenzo, AU - Izzo,Angelo A, Y1 - 2009/08/20/ PY - 2009/03/02/received PY - 2009/07/22/accepted PY - 2009/07/20/revised PY - 2009/8/20/entrez PY - 2009/8/20/pubmed PY - 2010/3/26/medline SP - 1111 EP - 21 JF - Journal of molecular medicine (Berlin, Germany) JO - J. Mol. Med. VL - 87 IS - 11 N2 - Inflammatory bowel disease affects millions of individuals; nevertheless, pharmacological treatment is disappointingly unsatisfactory. Cannabidiol, a safe and non-psychotropic ingredient of marijuana, exerts pharmacological effects (e.g., antioxidant) and mechanisms (e.g., inhibition of endocannabinoids enzymatic degradation) potentially beneficial for the inflamed gut. Thus, we investigated the effect of cannabidiol in a murine model of colitis. Colitis was induced in mice by intracolonic administration of dinitrobenzene sulfonic acid. Inflammation was assessed both macroscopically and histologically. In the inflamed colon, cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) were evaluated by Western blot, interleukin-1beta and interleukin-10 by ELISA, and endocannabinoids by isotope dilution liquid chromatography-mass spectrometry. Human colon adenocarcinoma (Caco-2) cells were used to evaluate the effect of cannabidiol on oxidative stress. Cannabidiol reduced colon injury, inducible iNOS (but not cyclooxygenase-2) expression, and interleukin-1beta, interleukin-10, and endocannabinoid changes associated with 2,4,6-dinitrobenzene sulfonic acid administration. In Caco-2 cells, cannabidiol reduced reactive oxygen species production and lipid peroxidation. In conclusion, cannabidiol, a likely safe compound, prevents experimental colitis in mice. SN - 1432-1440 UR - https://www.unboundmedicine.com/medline/citation/19690824/abstract/Cannabidiol_a_safe_and_non_psychotropic_ingredient_of_the_marijuana_plant_Cannabis_sativa_is_protective_in_a_murine_model_of_colitis_ L2 - https://dx.doi.org/10.1007/s00109-009-0512-x DB - PRIME DP - Unbound Medicine ER -