TY - JOUR T1 - Optimisation and validation of a new CE method for the determination of lansoprazole enantiomers in pharmaceuticals. AU - Nevado,Juon José Berzas, AU - Peñalvo,Gregorio Castañeda, AU - Sánchez,Juan Carlos Jiménez, AU - Mochón,Manuel Callejón, AU - Dorado,Rosa María Rodríguez, AU - Navarro,Mercedes Villar, PY - 2009/8/20/entrez PY - 2009/8/20/pubmed PY - 2010/1/6/medline SP - 2940 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 30 IS - 16 N2 - An analytical method based on CZE to determine lansoprazole enantiomers in pharmaceuticals was developed. The primary factors affecting its separation efficiency, which include chiral selector, pH, buffer concentration, capillary temperature and injection time, were optimised. The best results were obtained by using a background electrolyte consisting of 50 mM phosphate adjusted to pH 2.2, 12 mM beta-CD and 5 mM sodium sulphite, in combination with hydrodynamic injection and a 15 kV separation voltage. Detection limits were calculated from baseline noise and found to be 0.64 mg L(-1) for the R enantiomer and 0.72 mg L(-1) for the S enantiomer. The proposed method was used to analyse three different pharmaceutical preparations with recoveries of 91-102% of the label content. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/19691058/Optimisation_and_validation_of_a_new_CE_method_for_the_determination_of_lansoprazole_enantiomers_in_pharmaceuticals_ DB - PRIME DP - Unbound Medicine ER -