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In vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota.
J Agric Food Chem. 2009 Sep 23; 57(18):8598-606.JA

Abstract

Bifidobacterium adolescentis ATCC 15703, Bifidobacterium breve ATCC 15700, Bifidobacterium longum ATCC 15707, and human fecal microbiota were cultivated in vitro with d-xylose, l-arabinose, xylo-oligosaccharides (XOS), and arabinoxylo-oligosaccharides (AXOS) as carbon sources. The pH, formation of volatile fatty acids, and carbohydrate utilization profiles were followed. In the pure bifidobacteria cultures optical density and in the fecal slurries pressure and H(2) were also detected. A differing substrate preference was observed among the various bifidobacteria strains. B. adolescentis grew on XOS, slowly on d-xylose, but not on l-arabinose. In contrast, B. longum preferred l-arabinose and did not grow on pure d-xylose or XOS. Both strains were able to utilize AXOS but with differing strategies, since after the cleavage of l-arabinose B. adolescentis consumed the XOS formed, whereas B. longum fermented the l-arabinose released. B. breve grew poorly on all of the substrates provided. A bifidobacterial mixture and the fecal microbiota were able to utilize pure singly substituted AXOS almost completely, but pure AXOS with a doubly substituted xylose residue was fermented only by the fecal microbiota. Thus, AXOS appear to be potential candidates for slowly fermenting prebiotics, but their prebiotic effects may be dependent on the type of arabinose substitution and the presence of other carbohydrates.

Authors+Show Affiliations

Department of Applied Chemistry and Microbiology, FIN-00014 University of Helsinki, Finland. elena.pastell@helsinki.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19694435

Citation

Pastell, Helena, et al. "In Vitro Fermentation of Arabinoxylan-derived Carbohydrates By Bifidobacteria and Mixed Fecal Microbiota." Journal of Agricultural and Food Chemistry, vol. 57, no. 18, 2009, pp. 8598-606.
Pastell H, Westermann P, Meyer AS, et al. In vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota. J Agric Food Chem. 2009;57(18):8598-606.
Pastell, H., Westermann, P., Meyer, A. S., Tuomainen, P., & Tenkanen, M. (2009). In vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota. Journal of Agricultural and Food Chemistry, 57(18), 8598-606. https://doi.org/10.1021/jf901397b
Pastell H, et al. In Vitro Fermentation of Arabinoxylan-derived Carbohydrates By Bifidobacteria and Mixed Fecal Microbiota. J Agric Food Chem. 2009 Sep 23;57(18):8598-606. PubMed PMID: 19694435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota. AU - Pastell,Helena, AU - Westermann,Peter, AU - Meyer,Anne S, AU - Tuomainen,Päivi, AU - Tenkanen,Maija, PY - 2009/8/22/entrez PY - 2009/8/22/pubmed PY - 2010/1/29/medline SP - 8598 EP - 606 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 18 N2 - Bifidobacterium adolescentis ATCC 15703, Bifidobacterium breve ATCC 15700, Bifidobacterium longum ATCC 15707, and human fecal microbiota were cultivated in vitro with d-xylose, l-arabinose, xylo-oligosaccharides (XOS), and arabinoxylo-oligosaccharides (AXOS) as carbon sources. The pH, formation of volatile fatty acids, and carbohydrate utilization profiles were followed. In the pure bifidobacteria cultures optical density and in the fecal slurries pressure and H(2) were also detected. A differing substrate preference was observed among the various bifidobacteria strains. B. adolescentis grew on XOS, slowly on d-xylose, but not on l-arabinose. In contrast, B. longum preferred l-arabinose and did not grow on pure d-xylose or XOS. Both strains were able to utilize AXOS but with differing strategies, since after the cleavage of l-arabinose B. adolescentis consumed the XOS formed, whereas B. longum fermented the l-arabinose released. B. breve grew poorly on all of the substrates provided. A bifidobacterial mixture and the fecal microbiota were able to utilize pure singly substituted AXOS almost completely, but pure AXOS with a doubly substituted xylose residue was fermented only by the fecal microbiota. Thus, AXOS appear to be potential candidates for slowly fermenting prebiotics, but their prebiotic effects may be dependent on the type of arabinose substitution and the presence of other carbohydrates. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19694435/In_vitro_fermentation_of_arabinoxylan_derived_carbohydrates_by_bifidobacteria_and_mixed_fecal_microbiota_ L2 - https://doi.org/10.1021/jf901397b DB - PRIME DP - Unbound Medicine ER -