Select nutrients and their associated transporters are increased in the ovine uterus following early progesterone administration.Biol Reprod. 2010 Jan; 82(1):224-31.BR
The intrauterine milieu is a complex mixture of substances originating from serum and endometrium that support blastocyst growth and development. The present study identified alterations in glucose and amino acids in response to an early rise in progesterone (P4), which accelerates blastocyst growth and development. Bred ewes received daily injections of either corn oil (CO) vehicle or P4 from 36 h postmating (Day 0) to either Day 9 or Day 12. Another group of ewes received P4 to Day 8 and the antiprogestin mifepristone (RU486) from Day 8 to Day 12. The total amount of glucose, aspartate (acidic amino acid), arginine and lysine (basic amino acids), and citrulline, asparagine, serine, glutamine, beta-alanine, and alanine (neutral amino acids) was greater in uterine flushings from early P4- than CO-treated ewes on Day 9. On Day 12, only arginine and lysine were higher in uterine flushings from P4-treated ewes, whereas citrulline was reduced. Glucose transporters, SLC2A1 and SLC5A1, were increased in uterine luminal (LE) and superficial glandular (sGE) epithelia of early P4-treated ewes on Days 9 and 12 but were reduced in endometria from ewes treated with both P4 and RU486 (P4+RU). SLC7A2B, a transporter of basic amino acids, increased in LE/sGE of P4- versus CO-treated ewes on Day 12 but was reduced in P4+RU-treated ewes. Thus, select nutrients are increased in the uterine lumen by P4 concomitant with the upregulation of epithelial transporters for glucose and basic amino acids, suggesting that these nutrients stimulate blastocyst growth and development.