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Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy.
Biosens Bioelectron. 2009 Oct 15; 25(2):388-94.BB

Abstract

In current intracellular surface-enhanced Raman spectroscopy (SERS) measurements, gold or silver nanoparticles are delivered into living cells by "passive uptake". This procedure is time-consuming, could take up to several to twenties hours of incubation with nanoparticles in the culture medium. It is a less optimal method for certain applications such as high-throughput disease screening. Here, we present a method based on electroporation for fast delivery of silver nanoparticles into living cells for intracellular SERS spectroscopy. This new method for nanoparticle delivery averts the shortcoming of "passive uptake" and allows for quick acquisition of robust SERS spectra from living C666, A431, and CA46 cancer cell lines in our study. Our study also shows that the silver nanoparticles are localized only in the cell cytoplasm for electroporation delivery, while for "passive uptake", the nanoparticles have gone beyond the cytoplasm and into the nucleus. However, the whole-cell detection SERS spectra using electroporation delivery are more reproducible than for "passive uptake", thus are favored for practical applications. As a result, the process of SERS detection is accelerated significantly and the data reproducibility is improved as well, demonstrating great potential for biomedical applications, such as for high-throughput cancer cell screening.

Authors+Show Affiliations

Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19699079

Citation

Lin, Juqiang, et al. "Rapid Delivery of Silver Nanoparticles Into Living Cells By Electroporation for Surface-enhanced Raman Spectroscopy." Biosensors & Bioelectronics, vol. 25, no. 2, 2009, pp. 388-94.
Lin J, Chen R, Feng S, et al. Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy. Biosens Bioelectron. 2009;25(2):388-94.
Lin, J., Chen, R., Feng, S., Li, Y., Huang, Z., Xie, S., Yu, Y., Cheng, M., & Zeng, H. (2009). Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy. Biosensors & Bioelectronics, 25(2), 388-94. https://doi.org/10.1016/j.bios.2009.07.027
Lin J, et al. Rapid Delivery of Silver Nanoparticles Into Living Cells By Electroporation for Surface-enhanced Raman Spectroscopy. Biosens Bioelectron. 2009 Oct 15;25(2):388-94. PubMed PMID: 19699079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid delivery of silver nanoparticles into living cells by electroporation for surface-enhanced Raman spectroscopy. AU - Lin,Juqiang, AU - Chen,Rong, AU - Feng,Shangyuan, AU - Li,Yongzeng, AU - Huang,Zufang, AU - Xie,Shusen, AU - Yu,Yun, AU - Cheng,Min, AU - Zeng,Haishan, Y1 - 2009/08/03/ PY - 2009/06/03/received PY - 2009/07/22/revised PY - 2009/07/23/accepted PY - 2009/8/25/entrez PY - 2009/8/25/pubmed PY - 2009/12/16/medline SP - 388 EP - 94 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 2 N2 - In current intracellular surface-enhanced Raman spectroscopy (SERS) measurements, gold or silver nanoparticles are delivered into living cells by "passive uptake". This procedure is time-consuming, could take up to several to twenties hours of incubation with nanoparticles in the culture medium. It is a less optimal method for certain applications such as high-throughput disease screening. Here, we present a method based on electroporation for fast delivery of silver nanoparticles into living cells for intracellular SERS spectroscopy. This new method for nanoparticle delivery averts the shortcoming of "passive uptake" and allows for quick acquisition of robust SERS spectra from living C666, A431, and CA46 cancer cell lines in our study. Our study also shows that the silver nanoparticles are localized only in the cell cytoplasm for electroporation delivery, while for "passive uptake", the nanoparticles have gone beyond the cytoplasm and into the nucleus. However, the whole-cell detection SERS spectra using electroporation delivery are more reproducible than for "passive uptake", thus are favored for practical applications. As a result, the process of SERS detection is accelerated significantly and the data reproducibility is improved as well, demonstrating great potential for biomedical applications, such as for high-throughput cancer cell screening. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19699079/Rapid_delivery_of_silver_nanoparticles_into_living_cells_by_electroporation_for_surface_enhanced_Raman_spectroscopy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00397-2 DB - PRIME DP - Unbound Medicine ER -