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Nanoscale aggregation of cellular beta2-adrenergic receptors measured by plasmonic interactions of functionalized nanoparticles.
ACS Nano. 2009 Aug 25; 3(8):2329-39.AN

Abstract

Adrenergic signaling that controls the contraction of cardiac myocyte cells and the beating of the mammalian heart is initiated by ligand binding to adrenergic receptors contained in nanoscale multiprotein complexes at the cellular membrane. Here we demonstrate that the surface-enhanced Raman scattering (SERS) of functionalized silver nanoparticles can be used to report on the receptor aggregation state of specifically label beta(2)-adrenergic receptors on mouse cardiac myocyte cells. Furthermore, multimodal imaging including Raman, Rayleigh scattering, scanning electron microscopy, and luminescence imaging was combined to fully characterize the beta(2)-adrenergic receptor-mediated aggregation of silver nanoparticles on the membrane of cardiac myocytes. Scanning electron microscopy analysis reveals distinct SERS active clusters of between 10 and 70 nanoparticles per signaling domain from ultra-high-resolution images of beta(2)-adrenergic receptor clusters on the cellular membrane. These techniques can be generally applied to study the aggregation of other cell surface receptors and explore their distribution on cell surfaces.

Authors+Show Affiliations

Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19702324

Citation

Kennedy, David C., et al. "Nanoscale Aggregation of Cellular Beta2-adrenergic Receptors Measured By Plasmonic Interactions of Functionalized Nanoparticles." ACS Nano, vol. 3, no. 8, 2009, pp. 2329-39.
Kennedy DC, Tay LL, Lyn RK, et al. Nanoscale aggregation of cellular beta2-adrenergic receptors measured by plasmonic interactions of functionalized nanoparticles. ACS Nano. 2009;3(8):2329-39.
Kennedy, D. C., Tay, L. L., Lyn, R. K., Rouleau, Y., Hulse, J., & Pezacki, J. P. (2009). Nanoscale aggregation of cellular beta2-adrenergic receptors measured by plasmonic interactions of functionalized nanoparticles. ACS Nano, 3(8), 2329-39. https://doi.org/10.1021/nn900488u
Kennedy DC, et al. Nanoscale Aggregation of Cellular Beta2-adrenergic Receptors Measured By Plasmonic Interactions of Functionalized Nanoparticles. ACS Nano. 2009 Aug 25;3(8):2329-39. PubMed PMID: 19702324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanoscale aggregation of cellular beta2-adrenergic receptors measured by plasmonic interactions of functionalized nanoparticles. AU - Kennedy,David C, AU - Tay,Li-Lin, AU - Lyn,Rodney K, AU - Rouleau,Yanouchka, AU - Hulse,John, AU - Pezacki,John Paul, PY - 2009/8/26/entrez PY - 2009/8/26/pubmed PY - 2009/10/15/medline SP - 2329 EP - 39 JF - ACS nano JO - ACS Nano VL - 3 IS - 8 N2 - Adrenergic signaling that controls the contraction of cardiac myocyte cells and the beating of the mammalian heart is initiated by ligand binding to adrenergic receptors contained in nanoscale multiprotein complexes at the cellular membrane. Here we demonstrate that the surface-enhanced Raman scattering (SERS) of functionalized silver nanoparticles can be used to report on the receptor aggregation state of specifically label beta(2)-adrenergic receptors on mouse cardiac myocyte cells. Furthermore, multimodal imaging including Raman, Rayleigh scattering, scanning electron microscopy, and luminescence imaging was combined to fully characterize the beta(2)-adrenergic receptor-mediated aggregation of silver nanoparticles on the membrane of cardiac myocytes. Scanning electron microscopy analysis reveals distinct SERS active clusters of between 10 and 70 nanoparticles per signaling domain from ultra-high-resolution images of beta(2)-adrenergic receptor clusters on the cellular membrane. These techniques can be generally applied to study the aggregation of other cell surface receptors and explore their distribution on cell surfaces. SN - 1936-086X UR - https://www.unboundmedicine.com/medline/citation/19702324/Nanoscale_aggregation_of_cellular_beta2_adrenergic_receptors_measured_by_plasmonic_interactions_of_functionalized_nanoparticles_ L2 - https://doi.org/10.1021/nn900488u DB - PRIME DP - Unbound Medicine ER -