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Microbial diversity and community structure of a highly active anaerobic methane-oxidizing sulfate-reducing enrichment.
Environ Microbiol. 2009 Dec; 11(12):3223-32.EM

Abstract

Anaerobic oxidation of methane (AOM) is an important methane sink in the ocean but the microbes responsible for AOM are as yet resilient to cultivation. Here we describe the microbial analysis of an enrichment obtained in a novel submerged-membrane bioreactor system and capable of high-rate AOM (286 mumol g(dry weight)(-1) day(-1)) coupled to sulfate reduction. By constructing a clone library with subsequent sequencing and fluorescent in situ hybridization, we showed that the responsible methanotrophs belong to the ANME-2a subgroup of anaerobic methanotrophic archaea, and that sulfate reduction is most likely performed by sulfate-reducing bacteria commonly found in association with other ANME-related archaea in marine sediments. Another relevant portion of the bacterial sequences can be clustered within the order of Flavobacteriales but their role remains to be elucidated. Fluorescent in situ hybridization analyses showed that the ANME-2a cells occur as single cells without close contact to the bacterial syntrophic partner. Incubation with (13)C-labelled methane showed substantial incorporation of (13)C label in the bacterial C(16) fatty acids (bacterial; 20%, 44% and 49%) and in archaeal lipids, archaeol and hydroxyl-archaeol (21% and 20% respectively). The obtained data confirm that both archaea and bacteria are responsible for the anaerobic methane oxidation in a bioreactor enrichment inoculated with Eckernförde bay sediment.

Authors+Show Affiliations

Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19703218

Citation

Jagersma, G Christian, et al. "Microbial Diversity and Community Structure of a Highly Active Anaerobic Methane-oxidizing Sulfate-reducing Enrichment." Environmental Microbiology, vol. 11, no. 12, 2009, pp. 3223-32.
Jagersma GC, Meulepas RJ, Heikamp-de Jong I, et al. Microbial diversity and community structure of a highly active anaerobic methane-oxidizing sulfate-reducing enrichment. Environ Microbiol. 2009;11(12):3223-32.
Jagersma, G. C., Meulepas, R. J., Heikamp-de Jong, I., Gieteling, J., Klimiuk, A., Schouten, S., Damsté, J. S., Lens, P. N., & Stams, A. J. (2009). Microbial diversity and community structure of a highly active anaerobic methane-oxidizing sulfate-reducing enrichment. Environmental Microbiology, 11(12), 3223-32. https://doi.org/10.1111/j.1462-2920.2009.02036.x
Jagersma GC, et al. Microbial Diversity and Community Structure of a Highly Active Anaerobic Methane-oxidizing Sulfate-reducing Enrichment. Environ Microbiol. 2009;11(12):3223-32. PubMed PMID: 19703218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbial diversity and community structure of a highly active anaerobic methane-oxidizing sulfate-reducing enrichment. AU - Jagersma,G Christian, AU - Meulepas,Roel J W, AU - Heikamp-de Jong,Ineke, AU - Gieteling,Jarno, AU - Klimiuk,Adam, AU - Schouten,Stefan, AU - Damsté,Jaap S Sinninghe, AU - Lens,Piet N L, AU - Stams,Alfons J M, Y1 - 2009/08/24/ PY - 2009/8/26/entrez PY - 2009/8/26/pubmed PY - 2010/1/15/medline SP - 3223 EP - 32 JF - Environmental microbiology JO - Environ. Microbiol. VL - 11 IS - 12 N2 - Anaerobic oxidation of methane (AOM) is an important methane sink in the ocean but the microbes responsible for AOM are as yet resilient to cultivation. Here we describe the microbial analysis of an enrichment obtained in a novel submerged-membrane bioreactor system and capable of high-rate AOM (286 mumol g(dry weight)(-1) day(-1)) coupled to sulfate reduction. By constructing a clone library with subsequent sequencing and fluorescent in situ hybridization, we showed that the responsible methanotrophs belong to the ANME-2a subgroup of anaerobic methanotrophic archaea, and that sulfate reduction is most likely performed by sulfate-reducing bacteria commonly found in association with other ANME-related archaea in marine sediments. Another relevant portion of the bacterial sequences can be clustered within the order of Flavobacteriales but their role remains to be elucidated. Fluorescent in situ hybridization analyses showed that the ANME-2a cells occur as single cells without close contact to the bacterial syntrophic partner. Incubation with (13)C-labelled methane showed substantial incorporation of (13)C label in the bacterial C(16) fatty acids (bacterial; 20%, 44% and 49%) and in archaeal lipids, archaeol and hydroxyl-archaeol (21% and 20% respectively). The obtained data confirm that both archaea and bacteria are responsible for the anaerobic methane oxidation in a bioreactor enrichment inoculated with Eckernförde bay sediment. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/19703218/Microbial_diversity_and_community_structure_of_a_highly_active_anaerobic_methane_oxidizing_sulfate_reducing_enrichment_ L2 - https://doi.org/10.1111/j.1462-2920.2009.02036.x DB - PRIME DP - Unbound Medicine ER -