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Effects of Epimedium flavonoids on proliferation and differentiation of neural stem cells in vitro.
Neurol Res. 2010 Sep; 32(7):736-42.NR

Abstract

OBJECTIVE

The purpose of this study is to investigate the effects of Epimedium flavonoids (EF), which is extracted from a traditional Chinese Epimedium herb, and its effect on the proliferation and differentiation of neural stem cells (NSCs) in vitro.

METHODS

The single cells isolated from the hippocampi of 1 day old neonatal rats were cultured in a serum-free condition medium DMEM/F12 (1 : 1) with different concentrations of EF or 20 ng/ml epidermal growth factor (EGF) and 10 ng/ml basic fibroblast growth factor (bFGF). After 7 and 28 days, the neurospheres' diameters were measured. The formed neurospheres were cultured in the differentiation medium containing EF or 10% fetal bovine serum (FBS). After 12 hours and 7 days, immunofluorescent studies for nestin, Musashi-1, BrdU, beta-III-tubulin, NF-200 and GFAP were performed. The number and lengths of 10-15 axons of NF-200 immunopositive cells were measured.

RESULTS

The results showed that the isolated cells had the ability to propagate as neurospheres in the medium with 200 and 400 m g/ml EF, but without any EGF or bFGF, and the volume of neurospheres increase gradually from 7 to 28 days. In comparison with FBS control, the number of NF-200 positive neurons had significantly increased in the EF groups where the newborn neurons were morphologically more mature and able to migrate farther away from neurospheres than in the FBS control.

DISCUSSION

The results demonstrate that EF effectively promotes the proliferation and differentiation of NSCs in vitro, suggesting that EF may have new properties of regulating central nervous system function by neurogenesis.

Authors+Show Affiliations

Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19703337

Citation

Yao, Ruiqin, et al. "Effects of Epimedium Flavonoids On Proliferation and Differentiation of Neural Stem Cells in Vitro." Neurological Research, vol. 32, no. 7, 2010, pp. 736-42.
Yao R, Zhang L, Li X, et al. Effects of Epimedium flavonoids on proliferation and differentiation of neural stem cells in vitro. Neurol Res. 2010;32(7):736-42.
Yao, R., Zhang, L., Li, X., & Li, L. (2010). Effects of Epimedium flavonoids on proliferation and differentiation of neural stem cells in vitro. Neurological Research, 32(7), 736-42. https://doi.org/10.1179/174313209X459183
Yao R, et al. Effects of Epimedium Flavonoids On Proliferation and Differentiation of Neural Stem Cells in Vitro. Neurol Res. 2010;32(7):736-42. PubMed PMID: 19703337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Epimedium flavonoids on proliferation and differentiation of neural stem cells in vitro. AU - Yao,Ruiqin, AU - Zhang,Lan, AU - Li,Xiaoli, AU - Li,Lin, Y1 - 2009/08/21/ PY - 2009/8/26/entrez PY - 2009/8/26/pubmed PY - 2011/1/5/medline SP - 736 EP - 42 JF - Neurological research JO - Neurol. Res. VL - 32 IS - 7 N2 - OBJECTIVE: The purpose of this study is to investigate the effects of Epimedium flavonoids (EF), which is extracted from a traditional Chinese Epimedium herb, and its effect on the proliferation and differentiation of neural stem cells (NSCs) in vitro. METHODS: The single cells isolated from the hippocampi of 1 day old neonatal rats were cultured in a serum-free condition medium DMEM/F12 (1 : 1) with different concentrations of EF or 20 ng/ml epidermal growth factor (EGF) and 10 ng/ml basic fibroblast growth factor (bFGF). After 7 and 28 days, the neurospheres' diameters were measured. The formed neurospheres were cultured in the differentiation medium containing EF or 10% fetal bovine serum (FBS). After 12 hours and 7 days, immunofluorescent studies for nestin, Musashi-1, BrdU, beta-III-tubulin, NF-200 and GFAP were performed. The number and lengths of 10-15 axons of NF-200 immunopositive cells were measured. RESULTS: The results showed that the isolated cells had the ability to propagate as neurospheres in the medium with 200 and 400 m g/ml EF, but without any EGF or bFGF, and the volume of neurospheres increase gradually from 7 to 28 days. In comparison with FBS control, the number of NF-200 positive neurons had significantly increased in the EF groups where the newborn neurons were morphologically more mature and able to migrate farther away from neurospheres than in the FBS control. DISCUSSION: The results demonstrate that EF effectively promotes the proliferation and differentiation of NSCs in vitro, suggesting that EF may have new properties of regulating central nervous system function by neurogenesis. SN - 1743-1328 UR - https://www.unboundmedicine.com/medline/citation/19703337/Effects_of_Epimedium_flavonoids_on_proliferation_and_differentiation_of_neural_stem_cells_in_vitro_ L2 - http://www.tandfonline.com/doi/full/10.1179/174313209X459183 DB - PRIME DP - Unbound Medicine ER -