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Association of increased CCL5 and CXCL7 chemokine expression with neutrophil activation in severe stable COPD.
Thorax. 2009 Nov; 64(11):968-75.T

Abstract

BACKGROUND

Increased numbers of activated neutrophils have been reported in the bronchial mucosa of patients with stable chronic obstructive pulmonary disease (COPD), particularly in severe disease.

OBJECTIVES

To investigate the expression of neutrophilic chemokines and adhesion molecules in bronchial biopsies from patients with stable COPD of different severity (GOLD stages I-IV) compared with age-matched control subjects, smokers with normal lung function and never smokers.

METHODS

The expression of CCL5, CXCL1, 5, 6, 7 and 8, CXCR1, CXCR2, CD11b and CD44 was measured in the bronchial mucosa using immunohistochemistry, confocal immunofluorescence, real-time quantitative polymerase chain reaction (RT-QPCR) and Western blotting (WB).

RESULTS

The numbers of CCL5+ epithelial cells and CCL5+ and CXCL7+ immunostained cells were increased in the bronchial submucosa of patients with stable severe COPD compared with control never smokers and smokers with normal lung function. This was also confirmed at the level of mRNA expression. The numbers of CCL5+ cells in the submucosa of patients with COPD were 2-15 times higher than any other chemokines. There was no correlation between the number of these cells and the number of neutrophils in the bronchial submucosa. Compared with control smokers, the percentage of neutrophils co-expressing CD11b and CD44 receptors was significantly increased in the submucosa of patients with COPD.

CONCLUSION

The increased expression of CCL5 and CXCL7 in the bronchial mucosa of patients with stable COPD, together with an increased expression of extracellular matrix-binding receptors on neutrophils, may be involved in the pathogenesis of COPD.

Authors+Show Affiliations

Division of Pulmonary Disease and Laboratory of Cytoimmunopathology of Heart and Lung, Salvatore Maugeri Foundation, IRCCS, Veruno, Italy. antonino.distefano@fsm.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19703829

Citation

Di Stefano, A, et al. "Association of Increased CCL5 and CXCL7 Chemokine Expression With Neutrophil Activation in Severe Stable COPD." Thorax, vol. 64, no. 11, 2009, pp. 968-75.
Di Stefano A, Caramori G, Gnemmi I, et al. Association of increased CCL5 and CXCL7 chemokine expression with neutrophil activation in severe stable COPD. Thorax. 2009;64(11):968-75.
Di Stefano, A., Caramori, G., Gnemmi, I., Contoli, M., Bristot, L., Capelli, A., Ricciardolo, F. L., Magno, F., D'Anna, S. E., Zanini, A., Carbone, M., Sabatini, F., Usai, C., Brun, P., Chung, K. F., Barnes, P. J., Papi, A., Adcock, I. M., & Balbi, B. (2009). Association of increased CCL5 and CXCL7 chemokine expression with neutrophil activation in severe stable COPD. Thorax, 64(11), 968-75. https://doi.org/10.1136/thx.2009.113647
Di Stefano A, et al. Association of Increased CCL5 and CXCL7 Chemokine Expression With Neutrophil Activation in Severe Stable COPD. Thorax. 2009;64(11):968-75. PubMed PMID: 19703829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of increased CCL5 and CXCL7 chemokine expression with neutrophil activation in severe stable COPD. AU - Di Stefano,A, AU - Caramori,G, AU - Gnemmi,I, AU - Contoli,M, AU - Bristot,L, AU - Capelli,A, AU - Ricciardolo,F L M, AU - Magno,F, AU - D'Anna,S Ennio, AU - Zanini,A, AU - Carbone,M, AU - Sabatini,F, AU - Usai,C, AU - Brun,P, AU - Chung,K F, AU - Barnes,P J, AU - Papi,A, AU - Adcock,I M, AU - Balbi,B, Y1 - 2009/08/23/ PY - 2009/8/26/entrez PY - 2009/8/26/pubmed PY - 2010/3/27/medline SP - 968 EP - 75 JF - Thorax JO - Thorax VL - 64 IS - 11 N2 - BACKGROUND: Increased numbers of activated neutrophils have been reported in the bronchial mucosa of patients with stable chronic obstructive pulmonary disease (COPD), particularly in severe disease. OBJECTIVES: To investigate the expression of neutrophilic chemokines and adhesion molecules in bronchial biopsies from patients with stable COPD of different severity (GOLD stages I-IV) compared with age-matched control subjects, smokers with normal lung function and never smokers. METHODS: The expression of CCL5, CXCL1, 5, 6, 7 and 8, CXCR1, CXCR2, CD11b and CD44 was measured in the bronchial mucosa using immunohistochemistry, confocal immunofluorescence, real-time quantitative polymerase chain reaction (RT-QPCR) and Western blotting (WB). RESULTS: The numbers of CCL5+ epithelial cells and CCL5+ and CXCL7+ immunostained cells were increased in the bronchial submucosa of patients with stable severe COPD compared with control never smokers and smokers with normal lung function. This was also confirmed at the level of mRNA expression. The numbers of CCL5+ cells in the submucosa of patients with COPD were 2-15 times higher than any other chemokines. There was no correlation between the number of these cells and the number of neutrophils in the bronchial submucosa. Compared with control smokers, the percentage of neutrophils co-expressing CD11b and CD44 receptors was significantly increased in the submucosa of patients with COPD. CONCLUSION: The increased expression of CCL5 and CXCL7 in the bronchial mucosa of patients with stable COPD, together with an increased expression of extracellular matrix-binding receptors on neutrophils, may be involved in the pathogenesis of COPD. SN - 1468-3296 UR - https://www.unboundmedicine.com/medline/citation/19703829/Association_of_increased_CCL5_and_CXCL7_chemokine_expression_with_neutrophil_activation_in_severe_stable_COPD_ L2 - https://thorax.bmj.com/lookup/pmidlookup?view=long&pmid=19703829 DB - PRIME DP - Unbound Medicine ER -