TY - JOUR T1 - Detection of aminothiols through surface-assisted laser desorption/ionization mass spectrometry using mixed gold nanoparticles. AU - Chiang,Ni-Chen, AU - Chiang,Cheng-Kang, AU - Lin,Zong-Hong, AU - Chiu,Tai-Chia, AU - Chang,Huan-Tsung, PY - 2009/8/26/entrez PY - 2009/8/26/pubmed PY - 2009/10/29/medline SP - 3063 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 19 N2 - We have employed mixtures of two differently sized (average diameters: 3.5 and 14 nm) gold nanoparticles (Au NPs) as selective probes and matrices for the determination of aminothiols using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). When using 38 and 150 pM solutions of the 3.5- and 14-nm Au NPs, respectively, as the probe and matrix, SALDI-MS provided limits of detection (signal-to-noise ratio = 3) of 2, 20, and 44 nM for 1.0 mL solutions of glutathione (GSH), cysteine (Cys), and homocysteine, respectively. The signal intensities of these analytes varied by less than 20% for SALDI-MS analyses recorded over 50 sample spots; in contrast, they varied by as much as 60% when using a conventional matrix (2,5-dihydroxybenzoic acid). We validated the practicality of this approach - with its advantages of sensitivity, reproducibility, rapidity, and simplicity - through the analysis of GSH in MCF-7 cell lysates and Cys in plasma. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/19705381/Detection_of_aminothiols_through_surface_assisted_laser_desorption/ionization_mass_spectrometry_using_mixed_gold_nanoparticles_ L2 - https://doi.org/10.1002/rcm.4221 DB - PRIME DP - Unbound Medicine ER -