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'Radioresistant' CD4-CD8- intrathymic T cell precursors differentiate into mature CD4+CD8- and CD4-CD8+ T cells. Development of 'radioresistant' CD4-CD8- intrathymic T cell precursors.
Thymus. 1990 Mar; 15(2):65-78.T

Abstract

Using lectin (PNA) and monoclonal antibodies for Pgp-1, IL-2R, H-2k, CD3, and F23.1 (T cell receptor V beta 8), we characterized the 'radioresistant' CD4-CD8- double negative thymocytes at an early stage after 800 rad irradiation. Most of the CD4-CD8- cells on day 8 after irradiation expressed a high level of Thy-1, H-2k, and PNA, while a small proportion of these cells were CD3+ and/or F23.1+. The appearance of Pgp-1 and IL-2R on the 'radioresistant' double negative precursors was also sequentially examined from day 5 to day 9 after irradiation. The double negative thymocytes at day 5 expressed the highest level of Pgp-1 antigens and these cells gradually decreased in number from day 7 to day 9. By contrast, IL-2R was transiently expressed on the double negative cells on the day 7 and 8 after irradiation. These results indicate that progression of thymocyte development occurred within the CD4-CD8- thymocytes after irradiation. We further examined the homing ability of the double negative 'radioresistant' intrathymic T cell precursors to the periphery by intrathymic cell transplantation method. The double negative thymocytes proliferate and differentiate into CD4+CD8+ cells and CD4+CD8- cells but few CD4-CD8+ cells in the thymus, while only CD4-CD8+ cells were detected in the peripheral lymphoid organs 14 days after intrathymic transplantation of the double negative cells in the H-2 compatible Thy-1 congenic mice. These results suggest that the 'radioresistant' intrathymic precursors differentiate and mature in the thymus and migrate to the periphery.

Authors+Show Affiliations

Department of Immunology, Kyushu University, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1970676

Citation

Ayukawa, K, et al. "'Radioresistant' CD4-CD8- Intrathymic T Cell Precursors Differentiate Into Mature CD4+CD8- and CD4-CD8+ T Cells. Development of 'radioresistant' CD4-CD8- Intrathymic T Cell Precursors." Thymus, vol. 15, no. 2, 1990, pp. 65-78.
Ayukawa K, Tomooka S, Asano T, et al. 'Radioresistant' CD4-CD8- intrathymic T cell precursors differentiate into mature CD4+CD8- and CD4-CD8+ T cells. Development of 'radioresistant' CD4-CD8- intrathymic T cell precursors. Thymus. 1990;15(2):65-78.
Ayukawa, K., Tomooka, S., Asano, T., Taniguchi, K., Yoshikai, Y., & Nomoto, K. (1990). 'Radioresistant' CD4-CD8- intrathymic T cell precursors differentiate into mature CD4+CD8- and CD4-CD8+ T cells. Development of 'radioresistant' CD4-CD8- intrathymic T cell precursors. Thymus, 15(2), 65-78.
Ayukawa K, et al. 'Radioresistant' CD4-CD8- Intrathymic T Cell Precursors Differentiate Into Mature CD4+CD8- and CD4-CD8+ T Cells. Development of 'radioresistant' CD4-CD8- Intrathymic T Cell Precursors. Thymus. 1990;15(2):65-78. PubMed PMID: 1970676.
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TY - JOUR T1 - 'Radioresistant' CD4-CD8- intrathymic T cell precursors differentiate into mature CD4+CD8- and CD4-CD8+ T cells. Development of 'radioresistant' CD4-CD8- intrathymic T cell precursors. AU - Ayukawa,K, AU - Tomooka,S, AU - Asano,T, AU - Taniguchi,K, AU - Yoshikai,Y, AU - Nomoto,K, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 65 EP - 78 JF - Thymus JO - Thymus VL - 15 IS - 2 N2 - Using lectin (PNA) and monoclonal antibodies for Pgp-1, IL-2R, H-2k, CD3, and F23.1 (T cell receptor V beta 8), we characterized the 'radioresistant' CD4-CD8- double negative thymocytes at an early stage after 800 rad irradiation. Most of the CD4-CD8- cells on day 8 after irradiation expressed a high level of Thy-1, H-2k, and PNA, while a small proportion of these cells were CD3+ and/or F23.1+. The appearance of Pgp-1 and IL-2R on the 'radioresistant' double negative precursors was also sequentially examined from day 5 to day 9 after irradiation. The double negative thymocytes at day 5 expressed the highest level of Pgp-1 antigens and these cells gradually decreased in number from day 7 to day 9. By contrast, IL-2R was transiently expressed on the double negative cells on the day 7 and 8 after irradiation. These results indicate that progression of thymocyte development occurred within the CD4-CD8- thymocytes after irradiation. We further examined the homing ability of the double negative 'radioresistant' intrathymic T cell precursors to the periphery by intrathymic cell transplantation method. The double negative thymocytes proliferate and differentiate into CD4+CD8+ cells and CD4+CD8- cells but few CD4-CD8+ cells in the thymus, while only CD4-CD8+ cells were detected in the peripheral lymphoid organs 14 days after intrathymic transplantation of the double negative cells in the H-2 compatible Thy-1 congenic mice. These results suggest that the 'radioresistant' intrathymic precursors differentiate and mature in the thymus and migrate to the periphery. SN - 0165-6090 UR - https://www.unboundmedicine.com/medline/citation/1970676/'Radioresistant'_CD4_CD8__intrathymic_T_cell_precursors_differentiate_into_mature_CD4+CD8__and_CD4_CD8+_T_cells__Development_of_'radioresistant'_CD4_CD8__intrathymic_T_cell_precursors_ L2 - https://medlineplus.gov/stemcells.html DB - PRIME DP - Unbound Medicine ER -