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Role of vagal innervation in diurnal rhythm of intestinal peptide transporter 1 (PEPT1).
J Gastrointest Surg 2009; 13(11):1976-85JG

Abstract

BACKGROUND

Protein is absorbed predominantly as di/tripeptides via H(+)/peptide cotransporter-1 (PEPT1). We demonstrated previously diurnal variations in expression and function of duodenal and jejunal but not ileal PEPT1; neural regulation of this pattern is unexplored.

HYPOTHESIS

Complete abdominal vagotomy abolishes diurnal variations in gene expression and transport function of PEPT1.

METHODS

Twenty-four rats maintained in a 12-h light/dark room [6AM-6PM] underwent abdominal vagotomy; 24 other rats were controls. Four weeks later, mucosal levels of mRNA and protein were measured at 9AM, 3PM, 9PM, and 3AM (n = 6 each) by quantitative real-time PCR and Western blots, respectively; transporter-mediated uptake of dipeptide (Gly-Sar) was measured by the everted-sleeve technique.

RESULTS

Diurnal variation in mRNA, as in controls, was retained post-vagotomy in duodenum and jejunum (peak at 3PM, p < 0.05) but not in ileum. Diurnal variations in expression of protein and Gly-Sar uptake, however, were absent post-vagotomy (p > 0.3). Similar to controls, maximal uptake was in jejunum after vagotomy (V (max), nmol/cm/min: jejunum vs. duodenum and ileum; 163 vs. 88 and 71 at 3AM; p < 0.04); K (m) remained unchanged.

CONCLUSIONS

Vagal innervation appears to mediate in part diurnal variations in protein expression and transport function of PEPT1, but not diurnal variation in mRNA expression of PEPT1.

Authors+Show Affiliations

Gastrointestinal Research Unit and Department of Surgery, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19707837

Citation

Qandeel, Hisham G., et al. "Role of Vagal Innervation in Diurnal Rhythm of Intestinal Peptide Transporter 1 (PEPT1)." Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract, vol. 13, no. 11, 2009, pp. 1976-85.
Qandeel HG, Alonso F, Hernandez DJ, et al. Role of vagal innervation in diurnal rhythm of intestinal peptide transporter 1 (PEPT1). J Gastrointest Surg. 2009;13(11):1976-85.
Qandeel, H. G., Alonso, F., Hernandez, D. J., Duenes, J. A., Zheng, Y., Scow, J. S., & Sarr, M. G. (2009). Role of vagal innervation in diurnal rhythm of intestinal peptide transporter 1 (PEPT1). Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract, 13(11), pp. 1976-85. doi:10.1007/s11605-009-0984-6.
Qandeel HG, et al. Role of Vagal Innervation in Diurnal Rhythm of Intestinal Peptide Transporter 1 (PEPT1). J Gastrointest Surg. 2009;13(11):1976-85. PubMed PMID: 19707837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of vagal innervation in diurnal rhythm of intestinal peptide transporter 1 (PEPT1). AU - Qandeel,Hisham G, AU - Alonso,Fernando, AU - Hernandez,David J, AU - Duenes,Judith A, AU - Zheng,Ye, AU - Scow,Jeffrey S, AU - Sarr,Michael G, Y1 - 2009/08/26/ PY - 2009/05/22/received PY - 2009/07/24/accepted PY - 2009/8/27/entrez PY - 2009/8/27/pubmed PY - 2010/6/2/medline SP - 1976 EP - 85 JF - Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract JO - J. Gastrointest. Surg. VL - 13 IS - 11 N2 - BACKGROUND: Protein is absorbed predominantly as di/tripeptides via H(+)/peptide cotransporter-1 (PEPT1). We demonstrated previously diurnal variations in expression and function of duodenal and jejunal but not ileal PEPT1; neural regulation of this pattern is unexplored. HYPOTHESIS: Complete abdominal vagotomy abolishes diurnal variations in gene expression and transport function of PEPT1. METHODS: Twenty-four rats maintained in a 12-h light/dark room [6AM-6PM] underwent abdominal vagotomy; 24 other rats were controls. Four weeks later, mucosal levels of mRNA and protein were measured at 9AM, 3PM, 9PM, and 3AM (n = 6 each) by quantitative real-time PCR and Western blots, respectively; transporter-mediated uptake of dipeptide (Gly-Sar) was measured by the everted-sleeve technique. RESULTS: Diurnal variation in mRNA, as in controls, was retained post-vagotomy in duodenum and jejunum (peak at 3PM, p < 0.05) but not in ileum. Diurnal variations in expression of protein and Gly-Sar uptake, however, were absent post-vagotomy (p > 0.3). Similar to controls, maximal uptake was in jejunum after vagotomy (V (max), nmol/cm/min: jejunum vs. duodenum and ileum; 163 vs. 88 and 71 at 3AM; p < 0.04); K (m) remained unchanged. CONCLUSIONS: Vagal innervation appears to mediate in part diurnal variations in protein expression and transport function of PEPT1, but not diurnal variation in mRNA expression of PEPT1. SN - 1873-4626 UR - https://www.unboundmedicine.com/medline/citation/19707837/Role_of_vagal_innervation_in_diurnal_rhythm_of_intestinal_peptide_transporter_1__PEPT1__ L2 - https://dx.doi.org/10.1007/s11605-009-0984-6 DB - PRIME DP - Unbound Medicine ER -