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Panton-Valentine leukocidin-encoding bacteriophage and gene sequence variation in community-associated methicillin-resistant Staphylococcus aureus.
Clin Microbiol Infect 2010; 16(1):68-73CM

Abstract

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) often produce Panton-Valentine leukocin (PVL), which is encoded by two co-transcribed genes located on lysogenized bacteriophages. Six PVL-encoding temperate phages have been described and single nucleotide polymorphisms (SNPs) in the PVL genes have been reported. In the present study, 22 PVL-positive CA-MRSA isolates were chosen to reflect the diversity of multilocus sequence type (MLST) clonal complexes (CC) identified in our hospital. Isolates were characterized by antimicrobial resistance profile, staphylococcal cassette chromosome mec (SCCmec) and spa type, pulsed-field gel electrophoresis profile and MLST. Primers were designed to sequence the lukSF-PV genes. PVL-encoding phages were characterized using a PCR-based assay. SNPs were identified at seven locations in the lukSF-PV genes, which varied with S. aureus MLST lineage. One SNP was nonsynonymous. All CC80 and some CC1 isolates carried PhiSa2mw; CC8, CC88 and CC154 isolates harboured PVL-encoding elongated head-type phages; and some CC59 isolates harboured a PhiSa2958-like phage. Novel or variant phages were present in CC5 and some CC1 and CC59 isolates. The PVL gene sequence and the PVL-encoding phage varied with lineage. Further work is required to determine whether PVL sequence and/or phage variations result in biological differences.

Authors+Show Affiliations

Department of Infection, St Thomas' Hospital and King's College London, Lambeth Palace Road, London, UK. jonathan.otter@kcl.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19709067

Citation

Otter, J A., et al. "Panton-Valentine Leukocidin-encoding Bacteriophage and Gene Sequence Variation in Community-associated Methicillin-resistant Staphylococcus Aureus." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 16, no. 1, 2010, pp. 68-73.
Otter JA, Kearns AM, French GL, et al. Panton-Valentine leukocidin-encoding bacteriophage and gene sequence variation in community-associated methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect. 2010;16(1):68-73.
Otter, J. A., Kearns, A. M., French, G. L., & Ellington, M. J. (2010). Panton-Valentine leukocidin-encoding bacteriophage and gene sequence variation in community-associated methicillin-resistant Staphylococcus aureus. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 16(1), pp. 68-73. doi:10.1111/j.1469-0691.2009.02925.x.
Otter JA, et al. Panton-Valentine Leukocidin-encoding Bacteriophage and Gene Sequence Variation in Community-associated Methicillin-resistant Staphylococcus Aureus. Clin Microbiol Infect. 2010;16(1):68-73. PubMed PMID: 19709067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Panton-Valentine leukocidin-encoding bacteriophage and gene sequence variation in community-associated methicillin-resistant Staphylococcus aureus. AU - Otter,J A, AU - Kearns,A M, AU - French,G L, AU - Ellington,M J, PY - 2009/8/28/entrez PY - 2009/8/28/pubmed PY - 2010/3/11/medline SP - 68 EP - 73 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin. Microbiol. Infect. VL - 16 IS - 1 N2 - Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) often produce Panton-Valentine leukocin (PVL), which is encoded by two co-transcribed genes located on lysogenized bacteriophages. Six PVL-encoding temperate phages have been described and single nucleotide polymorphisms (SNPs) in the PVL genes have been reported. In the present study, 22 PVL-positive CA-MRSA isolates were chosen to reflect the diversity of multilocus sequence type (MLST) clonal complexes (CC) identified in our hospital. Isolates were characterized by antimicrobial resistance profile, staphylococcal cassette chromosome mec (SCCmec) and spa type, pulsed-field gel electrophoresis profile and MLST. Primers were designed to sequence the lukSF-PV genes. PVL-encoding phages were characterized using a PCR-based assay. SNPs were identified at seven locations in the lukSF-PV genes, which varied with S. aureus MLST lineage. One SNP was nonsynonymous. All CC80 and some CC1 isolates carried PhiSa2mw; CC8, CC88 and CC154 isolates harboured PVL-encoding elongated head-type phages; and some CC59 isolates harboured a PhiSa2958-like phage. Novel or variant phages were present in CC5 and some CC1 and CC59 isolates. The PVL gene sequence and the PVL-encoding phage varied with lineage. Further work is required to determine whether PVL sequence and/or phage variations result in biological differences. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/19709067/Panton_Valentine_leukocidin_encoding_bacteriophage_and_gene_sequence_variation_in_community_associated_methicillin_resistant_Staphylococcus_aureus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)61786-9 DB - PRIME DP - Unbound Medicine ER -