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Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis.
Clin Exp Allergy. 2010 Feb; 40(2):339-47.CE

Abstract

BACKGROUND

Component-resolved diagnosis (CRD) using microarray technology has recently been introduced into the field of clinical allergology.

OBJECTIVE

To further validate the use of CRD by microarray technology in allergy diagnosis.

METHODS

Thiry-seven patients allergic to birch pollen were included. The discriminative value of apple-specific IgE (sIgE), recombinant Mal d 1 (rMal d 1) sIgE, apple skin prick test and rMal d 1 on the microarray was assessed between patients with a birch-related oral allergy syndrome to apple (OAS(+), n=20) and healthy control individuals (HC, n=8) without a history of inhalant allergies or apple-induced OAS. An additional comparative analysis was carried out with individuals allergic to birch pollen allergy without OAS (OAS(-); n=17).

RESULTS

rMal d 1 coupled to the microarray constitutes a discriminative marker between OAS(+) and HC with a sensitivity 95% and a specificity of 100%. However, in parallel with the traditional sIgE assay, 15 out of 17 OAS(-) individuals (88%) also displayed IgE reactivity to rMal d 1 coupled to the microarray. OAS(-) individuals are more frequently sensitized to mite (about three to four times), cat and dog dander (about two to three times) and grass pollen (about 1.5 times) as compared with OAS(+) patients.

CONCLUSION

At first glance, CRD by microarray seems to be a reliable instrument in the diagnosis of apple-mediated OAS in birch pollen allergy. However, for discriminating between sensitization and a real allergy, micro-arrayed rMal d 1 offers no advantage over conventional quantification of rMal d 1 sIgE. Most interestingly, within a single run, birch pollen-allergic patients without OAS to apple were shown to display a broader sensitization to classical inhalant allergens than birch pollen-allergic patients with an apple-related OAS.

Authors+Show Affiliations

Departments of Immunology, Allergology, Rheumatology, Faculty of Medicine, University of Antwerp, Antwerpen, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19709127

Citation

Ebo, D G., et al. "Sensitization Profiles in Birch Pollen-allergic Patients With and Without Oral Allergy Syndrome to Apple: Lessons From Multiplexed Component-resolved Allergy Diagnosis." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 40, no. 2, 2010, pp. 339-47.
Ebo DG, Bridts CH, Verweij MM, et al. Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis. Clin Exp Allergy. 2010;40(2):339-47.
Ebo, D. G., Bridts, C. H., Verweij, M. M., De Knop, K. J., Hagendorens, M. M., De Clerck, L. S., & Stevens, W. J. (2010). Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 40(2), 339-47. https://doi.org/10.1111/j.1365-2222.2009.03345.x
Ebo DG, et al. Sensitization Profiles in Birch Pollen-allergic Patients With and Without Oral Allergy Syndrome to Apple: Lessons From Multiplexed Component-resolved Allergy Diagnosis. Clin Exp Allergy. 2010;40(2):339-47. PubMed PMID: 19709127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitization profiles in birch pollen-allergic patients with and without oral allergy syndrome to apple: lessons from multiplexed component-resolved allergy diagnosis. AU - Ebo,D G, AU - Bridts,C H, AU - Verweij,M M, AU - De Knop,K J, AU - Hagendorens,M M, AU - De Clerck,L S, AU - Stevens,W J, Y1 - 2009/08/26/ PY - 2009/8/28/entrez PY - 2009/8/28/pubmed PY - 2010/6/9/medline SP - 339 EP - 47 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 40 IS - 2 N2 - BACKGROUND: Component-resolved diagnosis (CRD) using microarray technology has recently been introduced into the field of clinical allergology. OBJECTIVE: To further validate the use of CRD by microarray technology in allergy diagnosis. METHODS: Thiry-seven patients allergic to birch pollen were included. The discriminative value of apple-specific IgE (sIgE), recombinant Mal d 1 (rMal d 1) sIgE, apple skin prick test and rMal d 1 on the microarray was assessed between patients with a birch-related oral allergy syndrome to apple (OAS(+), n=20) and healthy control individuals (HC, n=8) without a history of inhalant allergies or apple-induced OAS. An additional comparative analysis was carried out with individuals allergic to birch pollen allergy without OAS (OAS(-); n=17). RESULTS: rMal d 1 coupled to the microarray constitutes a discriminative marker between OAS(+) and HC with a sensitivity 95% and a specificity of 100%. However, in parallel with the traditional sIgE assay, 15 out of 17 OAS(-) individuals (88%) also displayed IgE reactivity to rMal d 1 coupled to the microarray. OAS(-) individuals are more frequently sensitized to mite (about three to four times), cat and dog dander (about two to three times) and grass pollen (about 1.5 times) as compared with OAS(+) patients. CONCLUSION: At first glance, CRD by microarray seems to be a reliable instrument in the diagnosis of apple-mediated OAS in birch pollen allergy. However, for discriminating between sensitization and a real allergy, micro-arrayed rMal d 1 offers no advantage over conventional quantification of rMal d 1 sIgE. Most interestingly, within a single run, birch pollen-allergic patients without OAS to apple were shown to display a broader sensitization to classical inhalant allergens than birch pollen-allergic patients with an apple-related OAS. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/19709127/Sensitization_profiles_in_birch_pollen_allergic_patients_with_and_without_oral_allergy_syndrome_to_apple:_lessons_from_multiplexed_component_resolved_allergy_diagnosis_ L2 - https://doi.org/10.1111/j.1365-2222.2009.03345.x DB - PRIME DP - Unbound Medicine ER -