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Induction of inducible CD4+CD25+Foxp3+ regulatory T lymphocytes by porcine reproductive and respiratory syndrome virus (PRRSV).
Vet Immunol Immunopathol. 2010 Feb 15; 133(2-4):170-82.VI

Abstract

Increases in numbers or activities of regulatory T lymphocytes (Tregs) have been linked to the establishments of several persistent infections. It has been previously shown that porcine reproductive and respiratory syndrome virus (PRRSV) can negatively modulate the host immune responses, resulting in persistent infection and secondary immunodeficiency. Recently, the existence of porcine CD4(+)CD25(+) Tregs has been demonstrated. We investigated the effect of PRRSV on the CD4(+)CD25(+) Tregs. The CD4(+)CD25(+)Foxp3(+) T lymphocytes in the peripheral blood mononuclear cells (PBMCs) were identified, using the anti-human anti-Foxp3 monoclonal antibody. In vitro culture of porcine PBMC in the presence of PRRSV, but not classical swine fever virus, significantly increased the numbers of Foxp3(+) lymphocytes, particularly in the CD4(+)CD25(high) subpopulation. The time-course study revealed that PRRSV significantly increased the numbers of viral-specific CD4(+)CD25(high)Foxp3(+) subpopulation in the culture starting from 12h through the end of the observation period. Consistent to the results obtained by flow cytometry, enhanced Foxp3 gene expression was observed in the PBMC cultured with PRRSV in a time-course manner. The presence of monocyte-derived DC in the co-culture significantly enhanced the induction of CD4(+)CD25(+) Foxp3(+) T lymphocytes. The PRRSV-induced CD4(+)CD25(high) T lymphocytes exhibited suppressive activity when co-cultured with PHA-activated, autologous peripheral blood leukocytes, indicating the suppressive activity of the PRRSV-specific Tregs. In addition, PRRSV exposure significantly increased the numbers of PRRSV-specific CD4(+)CD25(+)Foxp3(+) subpopulation in the PBMC of infected pigs at 10 days post-infection. In summary, the results indicated that PRRSV could increase the numbers of viral-specific, inducible regulatory T lymphocytes in the porcine PBMC, both in vitro and in vivo. The findings suggested the novel immunomodulatory mechanism induced by PRRSV.

Authors+Show Affiliations

Interdisciplinary Program of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19709757

Citation

Wongyanin, P, et al. "Induction of Inducible CD4+CD25+Foxp3+ Regulatory T Lymphocytes By Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)." Veterinary Immunology and Immunopathology, vol. 133, no. 2-4, 2010, pp. 170-82.
Wongyanin P, Buranapraditkun S, Chokeshai-Usaha K, et al. Induction of inducible CD4+CD25+Foxp3+ regulatory T lymphocytes by porcine reproductive and respiratory syndrome virus (PRRSV). Vet Immunol Immunopathol. 2010;133(2-4):170-82.
Wongyanin, P., Buranapraditkun, S., Chokeshai-Usaha, K., Thanawonguwech, R., & Suradhat, S. (2010). Induction of inducible CD4+CD25+Foxp3+ regulatory T lymphocytes by porcine reproductive and respiratory syndrome virus (PRRSV). Veterinary Immunology and Immunopathology, 133(2-4), 170-82. https://doi.org/10.1016/j.vetimm.2009.07.012
Wongyanin P, et al. Induction of Inducible CD4+CD25+Foxp3+ Regulatory T Lymphocytes By Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):170-82. PubMed PMID: 19709757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of inducible CD4+CD25+Foxp3+ regulatory T lymphocytes by porcine reproductive and respiratory syndrome virus (PRRSV). AU - Wongyanin,P, AU - Buranapraditkun,S, AU - Chokeshai-Usaha,K, AU - Thanawonguwech,R, AU - Suradhat,S, Y1 - 2009/08/03/ PY - 2008/11/06/received PY - 2009/07/01/revised PY - 2009/07/28/accepted PY - 2009/8/28/entrez PY - 2009/8/28/pubmed PY - 2010/4/17/medline SP - 170 EP - 82 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 133 IS - 2-4 N2 - Increases in numbers or activities of regulatory T lymphocytes (Tregs) have been linked to the establishments of several persistent infections. It has been previously shown that porcine reproductive and respiratory syndrome virus (PRRSV) can negatively modulate the host immune responses, resulting in persistent infection and secondary immunodeficiency. Recently, the existence of porcine CD4(+)CD25(+) Tregs has been demonstrated. We investigated the effect of PRRSV on the CD4(+)CD25(+) Tregs. The CD4(+)CD25(+)Foxp3(+) T lymphocytes in the peripheral blood mononuclear cells (PBMCs) were identified, using the anti-human anti-Foxp3 monoclonal antibody. In vitro culture of porcine PBMC in the presence of PRRSV, but not classical swine fever virus, significantly increased the numbers of Foxp3(+) lymphocytes, particularly in the CD4(+)CD25(high) subpopulation. The time-course study revealed that PRRSV significantly increased the numbers of viral-specific CD4(+)CD25(high)Foxp3(+) subpopulation in the culture starting from 12h through the end of the observation period. Consistent to the results obtained by flow cytometry, enhanced Foxp3 gene expression was observed in the PBMC cultured with PRRSV in a time-course manner. The presence of monocyte-derived DC in the co-culture significantly enhanced the induction of CD4(+)CD25(+) Foxp3(+) T lymphocytes. The PRRSV-induced CD4(+)CD25(high) T lymphocytes exhibited suppressive activity when co-cultured with PHA-activated, autologous peripheral blood leukocytes, indicating the suppressive activity of the PRRSV-specific Tregs. In addition, PRRSV exposure significantly increased the numbers of PRRSV-specific CD4(+)CD25(+)Foxp3(+) subpopulation in the PBMC of infected pigs at 10 days post-infection. In summary, the results indicated that PRRSV could increase the numbers of viral-specific, inducible regulatory T lymphocytes in the porcine PBMC, both in vitro and in vivo. The findings suggested the novel immunomodulatory mechanism induced by PRRSV. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/19709757/Induction_of_inducible_CD4+CD25+Foxp3+_regulatory_T_lymphocytes_by_porcine_reproductive_and_respiratory_syndrome_virus__PRRSV__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(09)00244-X DB - PRIME DP - Unbound Medicine ER -