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Enhanced angiotensin-mediated excitation of renal sympathetic nerve activity within the paraventricular nucleus of anesthetized rats with heart failure.
Am J Physiol Regul Integr Comp Physiol. 2009 Nov; 297(5):R1364-74.AJ

Abstract

Chronic heart failure (HF) is characterized by increased sympathetic drive. Enhanced angiotensin II (ANG II) activity may contribute to the increased sympathoexcitation under HF condition. The present study examined sympathoexcitation by 1) the effects of ANG II in the paraventricular nucleus (PVN) on renal sympathetic nerve activity (RSNA), and 2) the altered ANG II type 1 (AT(1)) receptor expression during HF. Left coronary artery ligation was used to induce HF. In the anesthetized Sprague-Dawley rats, microinjection of ANG II (0.05-1 nmol) into the PVN increased RSNA, mean arterial pressure (MAP), and heart rate (HR) in both sham-operated and HF rats. The responses of RSNA and HR were significantly enhanced in rats with HF compared with sham rats (RSNA: 64 +/- 8% vs. 33 +/- 4%, P < 0.05). Microinjection of AT(1) receptor antagonist losartan into the PVN produced a decrease of RSNA, MAP, and HR in both sham and HF rats. The RSNA and HR responses to losartan in HF rats were significantly greater (RSNA: -25 +/- 4% vs. -13 +/- 1%, P < 0.05). Using RT-PCR and Western blot analysis, we found that there were significant increases in the AT(1) receptor mRNA (Delta186 +/- 39%) and protein levels (Delta88 +/- 20%) in the PVN of rats with HF (P < 0.05). The immunofluorescence of AT(1) receptors was significantly higher in the PVN of rats with HF. These data support the conclusion that an increased angiotensinergic activity on sympathetic regulation, due to the upregulation of ANG II AT(1) receptors within the PVN, may contribute to the elevated sympathoexcitation that is observed during HF.

Authors+Show Affiliations

Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska 68198-5850, USA. hzheng@unmc.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19710393

Citation

Zheng, Hong, et al. "Enhanced Angiotensin-mediated Excitation of Renal Sympathetic Nerve Activity Within the Paraventricular Nucleus of Anesthetized Rats With Heart Failure." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 297, no. 5, 2009, pp. R1364-74.
Zheng H, Li YF, Wang W, et al. Enhanced angiotensin-mediated excitation of renal sympathetic nerve activity within the paraventricular nucleus of anesthetized rats with heart failure. Am J Physiol Regul Integr Comp Physiol. 2009;297(5):R1364-74.
Zheng, H., Li, Y. F., Wang, W., & Patel, K. P. (2009). Enhanced angiotensin-mediated excitation of renal sympathetic nerve activity within the paraventricular nucleus of anesthetized rats with heart failure. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 297(5), R1364-74. https://doi.org/10.1152/ajpregu.00149.2009
Zheng H, et al. Enhanced Angiotensin-mediated Excitation of Renal Sympathetic Nerve Activity Within the Paraventricular Nucleus of Anesthetized Rats With Heart Failure. Am J Physiol Regul Integr Comp Physiol. 2009;297(5):R1364-74. PubMed PMID: 19710393.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced angiotensin-mediated excitation of renal sympathetic nerve activity within the paraventricular nucleus of anesthetized rats with heart failure. AU - Zheng,Hong, AU - Li,Yi-Fan, AU - Wang,Wei, AU - Patel,Kaushik P, Y1 - 2009/08/26/ PY - 2009/8/28/entrez PY - 2009/8/28/pubmed PY - 2009/12/16/medline SP - R1364 EP - 74 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am J Physiol Regul Integr Comp Physiol VL - 297 IS - 5 N2 - Chronic heart failure (HF) is characterized by increased sympathetic drive. Enhanced angiotensin II (ANG II) activity may contribute to the increased sympathoexcitation under HF condition. The present study examined sympathoexcitation by 1) the effects of ANG II in the paraventricular nucleus (PVN) on renal sympathetic nerve activity (RSNA), and 2) the altered ANG II type 1 (AT(1)) receptor expression during HF. Left coronary artery ligation was used to induce HF. In the anesthetized Sprague-Dawley rats, microinjection of ANG II (0.05-1 nmol) into the PVN increased RSNA, mean arterial pressure (MAP), and heart rate (HR) in both sham-operated and HF rats. The responses of RSNA and HR were significantly enhanced in rats with HF compared with sham rats (RSNA: 64 +/- 8% vs. 33 +/- 4%, P < 0.05). Microinjection of AT(1) receptor antagonist losartan into the PVN produced a decrease of RSNA, MAP, and HR in both sham and HF rats. The RSNA and HR responses to losartan in HF rats were significantly greater (RSNA: -25 +/- 4% vs. -13 +/- 1%, P < 0.05). Using RT-PCR and Western blot analysis, we found that there were significant increases in the AT(1) receptor mRNA (Delta186 +/- 39%) and protein levels (Delta88 +/- 20%) in the PVN of rats with HF (P < 0.05). The immunofluorescence of AT(1) receptors was significantly higher in the PVN of rats with HF. These data support the conclusion that an increased angiotensinergic activity on sympathetic regulation, due to the upregulation of ANG II AT(1) receptors within the PVN, may contribute to the elevated sympathoexcitation that is observed during HF. SN - 1522-1490 UR - https://www.unboundmedicine.com/medline/citation/19710393/Enhanced_angiotensin_mediated_excitation_of_renal_sympathetic_nerve_activity_within_the_paraventricular_nucleus_of_anesthetized_rats_with_heart_failure_ L2 - https://journals.physiology.org/doi/10.1152/ajpregu.00149.2009?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -