Tags

Type your tag names separated by a space and hit enter

Molecular monitoring of culturable bacteria from deep-sea sediment of the Nankai Trough, Leg 190 Ocean Drilling Program.
FEMS Microbiol Ecol. 2004 Jun 01; 48(3):357-67.FM

Abstract

Culturable bacteria were detected in deep-sea sediment samples collected from the Nankai Trough site 1173 (Ocean Drilling Program, ODP, Leg 190) at 4.15 m below the seafloor with 4791 m of overlying water. In this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing bacteria were enriched by using two different non-selective enrichment culture media. Culturable bacterial population shifts within the deep marine sediment enrichments were monitored by using denaturating gradient gel electrophoresis (DGGE). DGGE analysis revealed a decrease in the number of 16S rRNA gene fragments from high to low carbon concentrations, and from low to high dilution of inoculum, suggesting that fast-growing bacteria were numerically dominant in enrichment culture samples. The dominant 16S rRNA fragments observed in DGGE gels were assigned to the Firmicutes, Proteobacteria (gamma and delta subgroups) and Spirochaeta phyla. Continual sub-culture and purification resulted in two isolates which were phylogenetically identified as members of the genera Acetobacterium and Marinilactibacillus. Our results, which combine enrichment culturing with DGGE analysis, indicated that enrichment cultures derived from inoculum dilution and media with various concentrations of carbon could facilitate the detection and isolation of a greater number of environmentally relevant bacterial species than when using traditional enrichment techniques alone.

Authors+Show Affiliations

Institut Universitaire Européen de la Mer, CNRS UMR, Plouzané, France. toffin@jamstec.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19712305

Citation

Toffin, Laurent, et al. "Molecular Monitoring of Culturable Bacteria From Deep-sea Sediment of the Nankai Trough, Leg 190 Ocean Drilling Program." FEMS Microbiology Ecology, vol. 48, no. 3, 2004, pp. 357-67.
Toffin L, Webster G, Weightman AJ, et al. Molecular monitoring of culturable bacteria from deep-sea sediment of the Nankai Trough, Leg 190 Ocean Drilling Program. FEMS Microbiol Ecol. 2004;48(3):357-67.
Toffin, L., Webster, G., Weightman, A. J., Fry, J. C., & Prieur, D. (2004). Molecular monitoring of culturable bacteria from deep-sea sediment of the Nankai Trough, Leg 190 Ocean Drilling Program. FEMS Microbiology Ecology, 48(3), 357-67. https://doi.org/10.1016/j.femsec.2004.02.009
Toffin L, et al. Molecular Monitoring of Culturable Bacteria From Deep-sea Sediment of the Nankai Trough, Leg 190 Ocean Drilling Program. FEMS Microbiol Ecol. 2004 Jun 1;48(3):357-67. PubMed PMID: 19712305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular monitoring of culturable bacteria from deep-sea sediment of the Nankai Trough, Leg 190 Ocean Drilling Program. AU - Toffin,Laurent, AU - Webster,Gordon, AU - Weightman,Andrew J, AU - Fry,John C, AU - Prieur,Daniel, PY - 2009/8/29/entrez PY - 2004/6/1/pubmed PY - 2009/9/15/medline SP - 357 EP - 67 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 48 IS - 3 N2 - Culturable bacteria were detected in deep-sea sediment samples collected from the Nankai Trough site 1173 (Ocean Drilling Program, ODP, Leg 190) at 4.15 m below the seafloor with 4791 m of overlying water. In this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing bacteria were enriched by using two different non-selective enrichment culture media. Culturable bacterial population shifts within the deep marine sediment enrichments were monitored by using denaturating gradient gel electrophoresis (DGGE). DGGE analysis revealed a decrease in the number of 16S rRNA gene fragments from high to low carbon concentrations, and from low to high dilution of inoculum, suggesting that fast-growing bacteria were numerically dominant in enrichment culture samples. The dominant 16S rRNA fragments observed in DGGE gels were assigned to the Firmicutes, Proteobacteria (gamma and delta subgroups) and Spirochaeta phyla. Continual sub-culture and purification resulted in two isolates which were phylogenetically identified as members of the genera Acetobacterium and Marinilactibacillus. Our results, which combine enrichment culturing with DGGE analysis, indicated that enrichment cultures derived from inoculum dilution and media with various concentrations of carbon could facilitate the detection and isolation of a greater number of environmentally relevant bacterial species than when using traditional enrichment techniques alone. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/19712305/Molecular_monitoring_of_culturable_bacteria_from_deep_sea_sediment_of_the_Nankai_Trough_Leg_190_Ocean_Drilling_Program_ DB - PRIME DP - Unbound Medicine ER -