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Addition of n-3 fatty acids to a 4-hour lipid infusion does not affect insulin sensitivity, insulin secretion, or markers of oxidative stress in subjects with type 2 diabetes mellitus.
Metabolism. 2009 Dec; 58(12):1753-61.M

Abstract

Fatty acids (FA) can impair glucose metabolism to a varying degree depending on time of exposure and also of type of FA. Here we tested for acute effects of marine n-3 FA on insulin sensitivity, insulin secretion, energy metabolism, and oxidative stress. This was a randomized, double-blind, crossover study in 11 subjects with type 2 diabetes mellitus. A 4-hour lipid infusion (Intralipid [Fresenius Kabi, Halden, Norway], total of 384 mL) was compared with a similar lipid infusion partly replaced by Omegaven (Fresenius Kabi) that contributed a median of 0.1 g fish oil per kilogram body weight, amounting to 0.04 g/kg of marine n-3 FA. Insulin sensitivity was assessed by isoglycemic hyperinsulinemic clamps; insulin secretion (measured after the clamps), by C-peptide glucagon tests; and energy metabolism, by indirect calorimetry. Infusion of Omegaven increased the proportion of n-3 FA in plasma nonesterified fatty acids (NEFA) compared with Intralipid alone (20:5n-3: median, 1.5% [interquartile range, 0.6%] vs -0.2% [0.2%], P = .001; 22:6n-3: 0.8% [0.4%] vs -0.7% [0.2%], P = .001). However, glucose utilization was not affected; neither was insulin secretion or total energy production (P = .966, .210, and .423, respectively, for the differences between the lipid clamps). Omegaven tended to lower oxidation of fat (P = .062) compared with Intralipid only, correlating with the rise in individual n-3 NEFA (r = 0.627, P = .039). The effects of clamping on phospholipid FA composition, leptin, adiponectin, or F(2)-isoprostane concentrations were not affected by Omegaven. Enrichment of NEFA with n-3 FA during a 4-hour infusion of Intralipid failed to affect insulin sensitivity, insulin secretion, or markers of oxidative stress in subjects with type 2 diabetes mellitus.

Authors+Show Affiliations

Division of Clinical Nutrition, Department of Clinical Service, St. Olavs Hospital, N-7006 Trondheim, Norway. ingrid.mostad@stolav.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19716144

Citation

Mostad, Ingrid L., et al. "Addition of N-3 Fatty Acids to a 4-hour Lipid Infusion Does Not Affect Insulin Sensitivity, Insulin Secretion, or Markers of Oxidative Stress in Subjects With Type 2 Diabetes Mellitus." Metabolism: Clinical and Experimental, vol. 58, no. 12, 2009, pp. 1753-61.
Mostad IL, Bjerve KS, Basu S, et al. Addition of n-3 fatty acids to a 4-hour lipid infusion does not affect insulin sensitivity, insulin secretion, or markers of oxidative stress in subjects with type 2 diabetes mellitus. Metabolism. 2009;58(12):1753-61.
Mostad, I. L., Bjerve, K. S., Basu, S., Sutton, P., Frayn, K. N., & Grill, V. (2009). Addition of n-3 fatty acids to a 4-hour lipid infusion does not affect insulin sensitivity, insulin secretion, or markers of oxidative stress in subjects with type 2 diabetes mellitus. Metabolism: Clinical and Experimental, 58(12), 1753-61. https://doi.org/10.1016/j.metabol.2009.06.003
Mostad IL, et al. Addition of N-3 Fatty Acids to a 4-hour Lipid Infusion Does Not Affect Insulin Sensitivity, Insulin Secretion, or Markers of Oxidative Stress in Subjects With Type 2 Diabetes Mellitus. Metabolism. 2009;58(12):1753-61. PubMed PMID: 19716144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Addition of n-3 fatty acids to a 4-hour lipid infusion does not affect insulin sensitivity, insulin secretion, or markers of oxidative stress in subjects with type 2 diabetes mellitus. AU - Mostad,Ingrid L, AU - Bjerve,Kristian S, AU - Basu,Samar, AU - Sutton,Pauline, AU - Frayn,Keith N, AU - Grill,Valdemar, Y1 - 2009/08/27/ PY - 2009/04/05/received PY - 2009/06/10/revised PY - 2009/06/16/accepted PY - 2009/9/1/entrez PY - 2009/9/1/pubmed PY - 2009/12/16/medline SP - 1753 EP - 61 JF - Metabolism: clinical and experimental JO - Metabolism VL - 58 IS - 12 N2 - Fatty acids (FA) can impair glucose metabolism to a varying degree depending on time of exposure and also of type of FA. Here we tested for acute effects of marine n-3 FA on insulin sensitivity, insulin secretion, energy metabolism, and oxidative stress. This was a randomized, double-blind, crossover study in 11 subjects with type 2 diabetes mellitus. A 4-hour lipid infusion (Intralipid [Fresenius Kabi, Halden, Norway], total of 384 mL) was compared with a similar lipid infusion partly replaced by Omegaven (Fresenius Kabi) that contributed a median of 0.1 g fish oil per kilogram body weight, amounting to 0.04 g/kg of marine n-3 FA. Insulin sensitivity was assessed by isoglycemic hyperinsulinemic clamps; insulin secretion (measured after the clamps), by C-peptide glucagon tests; and energy metabolism, by indirect calorimetry. Infusion of Omegaven increased the proportion of n-3 FA in plasma nonesterified fatty acids (NEFA) compared with Intralipid alone (20:5n-3: median, 1.5% [interquartile range, 0.6%] vs -0.2% [0.2%], P = .001; 22:6n-3: 0.8% [0.4%] vs -0.7% [0.2%], P = .001). However, glucose utilization was not affected; neither was insulin secretion or total energy production (P = .966, .210, and .423, respectively, for the differences between the lipid clamps). Omegaven tended to lower oxidation of fat (P = .062) compared with Intralipid only, correlating with the rise in individual n-3 NEFA (r = 0.627, P = .039). The effects of clamping on phospholipid FA composition, leptin, adiponectin, or F(2)-isoprostane concentrations were not affected by Omegaven. Enrichment of NEFA with n-3 FA during a 4-hour infusion of Intralipid failed to affect insulin sensitivity, insulin secretion, or markers of oxidative stress in subjects with type 2 diabetes mellitus. SN - 1532-8600 UR - https://www.unboundmedicine.com/medline/citation/19716144/Addition_of_n_3_fatty_acids_to_a_4_hour_lipid_infusion_does_not_affect_insulin_sensitivity_insulin_secretion_or_markers_of_oxidative_stress_in_subjects_with_type_2_diabetes_mellitus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-0495(09)00248-0 DB - PRIME DP - Unbound Medicine ER -