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Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry.
Aquat Toxicol. 2009 Oct 04; 94(4):255-64.AT

Abstract

Copper sulphate is a common algaecide applied to ponds in order to keep phytoplanktonic blooms under control, especially those prone to cyanobacterial development. The copper toxicity was evaluated for two phytoplanktonic species with the same size and shape: one cyanobacteria Microcystis aeruginosa and one chlorophyceae Chlorella vulgaris. Under controlled conditions in order to keep free copper concentration constant, three bioassays were carried out: growth inhibition tests and two sets of flow cytometric analysis (autofluorescence and esterase activity inhibition). The results showed: (1) that regardless of the cell type, as copper concentrations increased, cell division rate and autofluorescence decreased, (2) a difference in sensitivity according to the length of exposure to copper: 24 or 48 h, and (3) that M. aeruginosa showed a greater sensitivity than C. vulgaris in the 24h copper exposure assay according to esterase activity data.

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Authors+Show Affiliations

Hadjoudja S
Université de Limoges, GRESE EA 4330, 123 avenue Albert Thomas 87060, Limoges, France.
Vignoles C
No affiliation info available
Deluchat V
No affiliation info available
Lenain JF
No affiliation info available
Le Jeune AH
No affiliation info available
Baudu M
No affiliation info available

MeSH

Biological AssayCell DivisionChlorella vulgarisCopperCopper SulfateEsterasesFlow CytometryMicrocystisTime FactorsToxicity Tests

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19716610

Citation

Hadjoudja, Souad, et al. "Short Term Copper Toxicity On Microcystis Aeruginosa and Chlorella Vulgaris Using Flow Cytometry." Aquatic Toxicology (Amsterdam, Netherlands), vol. 94, no. 4, 2009, pp. 255-64.
Hadjoudja S, Vignoles C, Deluchat V, et al. Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry. Aquat Toxicol. 2009;94(4):255-64.
Hadjoudja, S., Vignoles, C., Deluchat, V., Lenain, J. F., Le Jeune, A. H., & Baudu, M. (2009). Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry. Aquatic Toxicology (Amsterdam, Netherlands), 94(4), 255-64. https://doi.org/10.1016/j.aquatox.2009.07.007
Hadjoudja S, et al. Short Term Copper Toxicity On Microcystis Aeruginosa and Chlorella Vulgaris Using Flow Cytometry. Aquat Toxicol. 2009 Oct 4;94(4):255-64. PubMed PMID: 19716610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry. AU - Hadjoudja,Souad, AU - Vignoles,Chantal, AU - Deluchat,Véronique, AU - Lenain,Jean-François, AU - Le Jeune,Anne-Hélène, AU - Baudu,Michel, Y1 - 2009/07/17/ PY - 2009/02/20/received PY - 2009/07/07/revised PY - 2009/07/12/accepted PY - 2009/9/1/entrez PY - 2009/9/1/pubmed PY - 2010/3/6/medline SP - 255 EP - 64 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 94 IS - 4 N2 - Copper sulphate is a common algaecide applied to ponds in order to keep phytoplanktonic blooms under control, especially those prone to cyanobacterial development. The copper toxicity was evaluated for two phytoplanktonic species with the same size and shape: one cyanobacteria Microcystis aeruginosa and one chlorophyceae Chlorella vulgaris. Under controlled conditions in order to keep free copper concentration constant, three bioassays were carried out: growth inhibition tests and two sets of flow cytometric analysis (autofluorescence and esterase activity inhibition). The results showed: (1) that regardless of the cell type, as copper concentrations increased, cell division rate and autofluorescence decreased, (2) a difference in sensitivity according to the length of exposure to copper: 24 or 48 h, and (3) that M. aeruginosa showed a greater sensitivity than C. vulgaris in the 24h copper exposure assay according to esterase activity data. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/19716610/Short_term_copper_toxicity_on_Microcystis_aeruginosa_and_Chlorella_vulgaris_using_flow_cytometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(09)00241-0 DB - PRIME DP - Unbound Medicine ER -