Tags

Type your tag names separated by a space and hit enter

The roles of extracellular matrix proteins, apoptosis and c-kit positive cells in the pathogenesis of ureteropelvic junction obstruction.
J Pediatr Urol. 2010 Apr; 6(2):125-9.JP

Abstract

AIM

To investigate histopathological changes in ureteropelvic junction obstruction (UPJO) from an etiological perspective.

PATIENTS AND METHODS

Medical records of patients with UPJO were reviewed and pathological specimens collected. Nephrectomy materials from forensic autopsies were taken as controls. Specimens were assessed with light microscopy. Fibronectin, type 4 collagen, laminin, Bax and Bcl-2 expression for apoptosis, together with interstitial cells of Cajal determination with c-kit were determined immunohistochemically. Staining scores were evaluated semiquantitatively. Results were evaluated using Mann-Whitney U-test.

RESULTS

Control group comprised 14 children (median age, 3.5 years; 6 months-17 years). Study group comprised 22 children with UPJO (median age, 9 months; 1 month-10 years). Light microscopy revealed non-specific inflammation, epithelial proliferation and atrophy with fibrosis in the smooth muscle of the UPJ in all patients. Fibronectin, type 4 collagen and laminin were found to be significantly increased in UPJO at the intrafascicular space of smooth muscle and the matrix of stroma. Bcl-2 expression was increased in UPJO. c-Kit was unable to stain interstitial cells of Cajal, but staining for mast cells was significant.

CONCLUSIONS

High expression of fibronectin, laminin and type 4 collagen may indicate a relation to the pathogenesis of UPJO. Defective kidney morphogenesis, during branching and tubulogenesis of ureteric bud, may be responsible for this congenital pathology.

Authors+Show Affiliations

Fırat University Faculty of Medicine, Department of Pediatric Surgery, Elazığ, Turkey. skozel@firat.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19716769

Citation

Özel, S K., et al. "The Roles of Extracellular Matrix Proteins, Apoptosis and C-kit Positive Cells in the Pathogenesis of Ureteropelvic Junction Obstruction." Journal of Pediatric Urology, vol. 6, no. 2, 2010, pp. 125-9.
Özel SK, Emir H, Dervişoğlu S, et al. The roles of extracellular matrix proteins, apoptosis and c-kit positive cells in the pathogenesis of ureteropelvic junction obstruction. J Pediatr Urol. 2010;6(2):125-9.
Özel, S. K., Emir, H., Dervişoğlu, S., Akpolat, N., Şenel, B., Kazez, A., Söylet, Y., Çetin, G., Danişmend, N., & Büyükünal, S. N. (2010). The roles of extracellular matrix proteins, apoptosis and c-kit positive cells in the pathogenesis of ureteropelvic junction obstruction. Journal of Pediatric Urology, 6(2), 125-9. https://doi.org/10.1016/j.jpurol.2009.07.011
Özel SK, et al. The Roles of Extracellular Matrix Proteins, Apoptosis and C-kit Positive Cells in the Pathogenesis of Ureteropelvic Junction Obstruction. J Pediatr Urol. 2010;6(2):125-9. PubMed PMID: 19716769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The roles of extracellular matrix proteins, apoptosis and c-kit positive cells in the pathogenesis of ureteropelvic junction obstruction. AU - Özel,S K, AU - Emir,H, AU - Dervişoğlu,S, AU - Akpolat,N, AU - Şenel,B, AU - Kazez,A, AU - Söylet,Y, AU - Çetin,G, AU - Danişmend,N, AU - Büyükünal,S N C, Y1 - 2009/08/28/ PY - 2009/04/07/received PY - 2009/07/23/accepted PY - 2009/9/1/entrez PY - 2009/9/1/pubmed PY - 2012/8/7/medline SP - 125 EP - 9 JF - Journal of pediatric urology JO - J Pediatr Urol VL - 6 IS - 2 N2 - AIM: To investigate histopathological changes in ureteropelvic junction obstruction (UPJO) from an etiological perspective. PATIENTS AND METHODS: Medical records of patients with UPJO were reviewed and pathological specimens collected. Nephrectomy materials from forensic autopsies were taken as controls. Specimens were assessed with light microscopy. Fibronectin, type 4 collagen, laminin, Bax and Bcl-2 expression for apoptosis, together with interstitial cells of Cajal determination with c-kit were determined immunohistochemically. Staining scores were evaluated semiquantitatively. Results were evaluated using Mann-Whitney U-test. RESULTS: Control group comprised 14 children (median age, 3.5 years; 6 months-17 years). Study group comprised 22 children with UPJO (median age, 9 months; 1 month-10 years). Light microscopy revealed non-specific inflammation, epithelial proliferation and atrophy with fibrosis in the smooth muscle of the UPJ in all patients. Fibronectin, type 4 collagen and laminin were found to be significantly increased in UPJO at the intrafascicular space of smooth muscle and the matrix of stroma. Bcl-2 expression was increased in UPJO. c-Kit was unable to stain interstitial cells of Cajal, but staining for mast cells was significant. CONCLUSIONS: High expression of fibronectin, laminin and type 4 collagen may indicate a relation to the pathogenesis of UPJO. Defective kidney morphogenesis, during branching and tubulogenesis of ureteric bud, may be responsible for this congenital pathology. SN - 1873-4898 UR - https://www.unboundmedicine.com/medline/citation/19716769/The_roles_of_extracellular_matrix_proteins_apoptosis_and_c_kit_positive_cells_in_the_pathogenesis_of_ureteropelvic_junction_obstruction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1477-5131(09)00424-0 DB - PRIME DP - Unbound Medicine ER -